The virology laboratory in cytomegalovirus infection. Current possibilities;El laboratorio de virologia en la infeccion por citomegalovirus. Posibilidades actuales

Enfermedades Infecciosas y Microbiologia Clinica

Volumn 15, Issue SUPPL. 2, 1997, Pages 69-76

  Rabella, Núria ^a   Pérez, José Luis ^b   Pumarola, Tomás ^c  

^a HOSPITAL DE LA SANTA CREU I SANT PAU   (Spain)

^b UB   (Spain)

^c HOSPITAL CLÍNIC   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIGEN DETECTION; CYTOMEGALOVIRUS INFECTION; DIAGNOSTIC ACCURACY; GOOD LABORATORY PRACTICE; HUMAN; NONHUMAN; REVIEW; SPAIN; VIRUS CULTURE; ANIMAL; CELL CULTURE; CYTOMEGALIC INCLUSION BODY DISEASE; CYTOMEGALOVIRUS; EPIDEMIOLOGY; IMMUNOLOGY; ISOLATION AND PURIFICATION; PHYSIOLOGY; POLYMERASE CHAIN REACTION;

VIRUS ANTIBODY; VIRUS ANTIGEN; VIRUS DNA; VIRUS PROTEIN;

ANIMALS; ANTIBODIES, VIRAL; ANTIGENS, VIRAL; CELLS, CULTURED; CYTOMEGALOVIRUS; CYTOMEGALOVIRUS INFECTIONS; DNA, VIRAL; HUMANS; POLYMERASE CHAIN REACTION; SEROEPIDEMIOLOGIC STUDIES; VIRAL PROTEINS; VIRUS CULTIVATION;

EID: 0031008783     PISSN: 0213005X     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Review

References (63)

1. Meyers JD, Ljungman P, Fisher LD. Cytomegalovirus excretion as predictor of cytomegalovirus diseases after marrow transplantation: importance of cytomegalovirus viremia. J Infect Dis 1990; 162: 373-380.
2. Saltzman RL, Quirk MR, Jordan MC. High levels of circulating cytomegalo-virus DNA reflect visceral organ disease in viremic immunosuppressed patients other than marrow recipients. J Clin Invest 1992; 90: 1.382-1.388.
3. Niubó J, Viqueira M, García A, Manito N, Pérez JL, Martín R. La muestra por citomegalovirus en trasplantados hepaticos y cardíacos. Med Clin (Barc). En prensa.
4. Virological Diagnosis and Infections in Cells and Tissues. En: Monto Ho, ed. Cytomegalovirus. Biology and Infection, 2a. ed. Nueva York: Plenum Medical Book Co., 1991; 75-79.
5. Espy MJ, Arlo DW, Ilstrup DM, Smith TF. Effect of treatment of shell vial cell cultures with dimethyl sulfoxide and dexamethasone of detection of cytomegalovirus. J Clin Microbiol 1988; 26: 1.091-1.093.
6. West PG, Hartwig RA, Baker WW. Comparison of sentitivities of three commercial MRC-5 cell lines grown in shell vials to cytomegalovirus and responses to enhancing agents. J Clin Microbiol 1992; 30: 557-560.
7. Miller H, McCulloch B, Landini MP, Rossier E. Comparison of immunoblotting with other serological methods and virus isolation for the early detection of primary cytomegalovirus infection in allograft recipients. J Clin Microbiol 1989; 27: 2.672-2.677.
8. Drew WL. Herpesviridae: Cytomegalovirus. En: Lennette EH, Halonen P, Murphy FA, eds. Laboratory Diagnosis of infections. Principles and Practice. Viral, Rickettsial, and Chlamydial Diseases. Nueva York: Springer-Verlag Inc, 1988; 2: 247-260.
9. Griffiths PD, Panjwani DD, Stirk PR, Ball MG, Ganczakowski M, Blancklock HA et al. Rapid diagnosis of cytomegalovirus infection in immunocompromised patients by detection of early antigen fluorescent foci. Lancet 1984; 2: 1.242-1.245.
10. Gleaves CA, Smith TF, Shuster EA, Pearson GR. Rapid detection of cytomegalovirus in MRC-5 cells inoculated with urine especimens by using low-speed centrifugation and monoclonal antibody to an early antigen. J Clin Microbiol 1984; 19: 917-919.
11. Jespersen DJ, Drew WL, Gleaves CA, Meyers JD, Warford AL, Smith TF. Multisite evaluation of a monoclonal antibody reagent (Syva) for rapid diagnosis of cytomegalovirus in the shell vial assay. J Clin Microbiol 1989; 27: 1.502-1.505.
12. Agha SA, Coleman JC, Selwyn S, Mahmoud LA, Abd-Elaal AM. Combined use of sonication and monoclonal antibodies for the detection of early and late cytomegalovirus antigens in centrifugation cultures. J Virol Methods 1988; 22: 41-50.
13. Gleaves CA, Hursh DA, Meyers JD. Detection of cytomegalovirus in clinical specimens by centrifugation culture with a nonhuman cell line. J Clin Microbiol 1992; 30: 1.045-1.048.
14. Gerna G, Revello MG, Percivalle E, Zavattoni M, Parea M, Battaglia M. Quantification of human cytomegalovirus viremia by using monoclonal antibodies to different viral proteins. J Clin Microbiol 1990; 28: 2.681-2.688.
15. Buller RS, Bailey TC, Ettinger NA, Keener M, Langlois T, Miller JP et al. Use of modified shell vial technique to quantitate cytomegalovirus viremia in a population of solid-organ transplant recipients. J Clin Microbiol 1992; 30: 2.620-2.624.
16. Slavin MA, Gleaves CA, Schoch HG, Bowden RA. Quantification of cytomegalovirus in bronchoalveolar lavage fluid after allogeneic marrow transplantation by centrifugation culture. J Clin Microbiol 1992; 30: 2.776-2.779.
17. Klevjer-Anderson P. Diagnostic significance of CMV serology. Clin Microbiol Newsletter 1987; 9: 36-38.
18. Faix RG. Cytomegalovirus antigenic heterogeneity can cause false-negative results in indirect hemagglutination and complement fixation antibody assays. J Clin Microbiol 1985; 22: 768-771.
19. Adler SP, McVoy M, Biro VG, Britt W. Detection of cytomegalovirus antibody with latex agglutination. J Clin Microbiol 1985; 22: 68-70.
20. Kangro HO. Cytomegalovirus serology: does it give the answer? Serodiagn Immunother 1987; 1: 91-99.
21. Stinski MF. Cytomegalovirus and its replication. En: Field BX, Knipe DM, eds. Virology, 2a ed. Nueva York: Raven Press, 1990; 1.959-1.980.
22. Van der Bij W, Torensma R, van Son WJ, Anema J, Schirm J, Tegzess AM et al. Rapid immunodiagnosis of active cytomegalovirus infection by monoclonal antibody staining of blood leucotytes. J Med Virol 1988; 25: 179-188.
23. Dankner WM, McCutchan JA, Richman DD, Hirata K, Spector SA. Localization of human cytomegalovirus in peripheral blood leukocytes by in situ hibridization. J Infect Dis 1990; 161: 31-36.
24. Gerna G, Revello MG, Percivalle E, Morini F. Comparison of different immunostaining techniques and monoclonal antibodies to the lower matrix phosphoprotein (ppG5) for optimal quantitation of human cytomegalovirus antigenemia. J Clin Microbiol 1992; 1.232-1.237.
25. Van der Bij W, van Son WJ, van den Berg AP, Tegzess AM, Torensma R, The TH. Cytomegalovirus antigenemia: rapid diagnosis and relationship with CMV-associatcd clinical syndromes in renal allograft recipients. Transplant Proc 1989; 21: 2.061-2.064.
26. Gerna G, Zipeto D, Parea M, Revello MG, Silini E, Percivalle E et al. Monitoring of human cytomegalovirus infections and ganciclovir treatment in heart transplant recipients by determination of viremia, antigenemia and DNAemia. J Infect Dis 1991; 164: 488-498.
27. Erice A, Holm MA, Gill P, Henry S, Dirksen CL, Dunn DL et al. Cytomegalovirus antigenemia assay is more sensitive than the shell vial cultures for rapid detection of CMV in polymorphonuclear blood leukocytes. J Clin Microbiol 1992; 30: 2.822-2.825.
28. Koskinen PK, Nieminen MS, Mattila SP, Hayry PJ. Lautenschlager IT. The correlation between symptomatic CMV infection and CMV antigenemia in heart allografts recipients. Transplantation 1993; 55: 547-551.
29. Van der Bij W, van Dijk RB, van Son WJ, Torensma R, Prenger KB, Prop J et al. Antigen test for early diagnosis of active cytomegalovirus infection in heart transplant recipients. J Heart Transplant 1988; 7: 106-110.
30. The TH, van der Bij W, van den Berg AP, van der Giessen M, Weits J, Sprenger HJ, van Son WJ. Cytomegalovirus antigenemia. Rev Infect Dis 1990; 12 (Supl 7): 737-744.
31. The TH, van der Ploeg M, van den Berg AP, Vliegler AM, van der Giessen, van Son WJ. Direct detection of cytomegalovirus in peripheral blood leukocytes. A review of the antigenemia assay and polymerase chain reaction. Transplantation 1992; 54: 193-198.
32. Van den Berg AP, Klompmaker IJ, Haagsma EB, Scholten-Sampson A, Bijleveld CMA, Schirm J et al. Antigenemia in the diagnosis and monitoring of active cytomegalovirus infection after liver transplantation. J Infect Dis 1991; 164: 265-270.
33. Storch GA, Gaudreault-Kleener M, Welby PC. Comparison of heparin and EDTA transport tubes for detection of cytomegalovirus in leukocytes by shell vial assay, pp65 antigenemia and PCR. J Clin Microbiol 1994; 12 (Supl 7): S737-S744.
34. Van der Giessen M, The TH, van Son WJ. Cytomegalovirus antigenemia assay [carta]. J Clin Microbiol 1991; 29: 2.909-2.910.
35. Niubó J, Pérez JL, Carvajal A, Ardanuy C, Martin R. Effect of delayed processing of the blood samples on performance of cytomegalovirus antigenemia assay. J Clin Microbiol 1994; 32: 1.119-1.120.
36. García A, Niubó J, Benítez MA, Viqueira M, Pérez JL. Comparison of two leukocyte extraction methods for cytomegalovirus antigenemia assay. J Clin Microbiol 1996; 34: 182-184.
37. Boeckh M, Woogerd PM, Stevens-Ayers T, Ray GC, Bowden RA. Factors influencing detection of quantitative cytomegalovirus antigenemia. J Clin Microbiol 1994; 32: 832-833.
38. Pérez JL, Oña M, Niubó J, Villar H, Melón S, García A et al. Comparison of several fixation methods of cytomegalovirus antigenemia assay. J Clin Microbiol 1995; 33: 1.646-1.649.
39. Pérez JL, Niubó J, Ardanuy C, Carvajal A, García A, Salvá J et al. Comparison of three commercially available monoclonal antibodies directed against pp65 antigen for cytomegalovirus antigenemia assay. Diagn Microbiol Infect Dis 1995; 21: 21-25.
40. Van der Bij W, Schirm J, Torensma R, van Son WJ, Tegzees AM, The TH. Comparison between viremia and antigenemia for detection of cytomegalovirus in blood. J Clin Microbiol 1988; 26: 2.531-2.535.
41. Van den Berg AP, van der Bij W, van Son WJ, Anema J, van der Giessen M, Schirm J et al. Cytomcgalovirus antigenemia as a useful marker of symptomatic cytomegalovirus infection after renal transplantation: a report of 130 consecutive patients. Transplantation 1989; 48: 991-995.
42. Van den Berg AP, van Son WJ, van der Bij W, van der Giessen M, The TH. Early identification of patients at high risk of severe cytomegalovirus disease after renal transplantation using immediate early antigenemia assay. Transplant Proc 1990; 22: 1.800-1.802.
43. Miller H, Rosier E, Milk R, Thomas C. Prospective study of cytomegalovirus antigenemia in allograft recipients. J Clin Microbiol 1991; 29: 1.054-1.055.
44. Revello MG, Pcrcivalle E, Zavattoni M, Parca M, Grossi P, Gerna G. Detection of human cytomegalovirus immediate early antigen in leukocytes as a marker of viremia in immunocompromised patients. J Med Virol 1989; 29: 88-93.
45. Gondo H, Minematsu T, Harada M, Akashi K, Hatyashi S, Taniguchi S et al. Cytomegalovirus antigenemia for rapid diagnosis and monitoring of CMV disease after bone marrow transplantation. Br J Haematol 1994; 86: 130-137.
46. Grossi P, Minoli L, Percivalle E, Irish W, Vigano M, Gerna G. Clinical and virological monitoring of human cytomegalovirus infection in 294 heart transplant recipients. Transplantation 1995; 59: 847-851.
47. Lazzaroto T, Campisi B, Galli S, Guglielmi P, Landini MP. A quantitative test (CMV-hybrid capture) for the detection of CMV DNA in the blood of immunocompromised patients [resumen P102] 5th International Cytomegalovirus Conference. Estocolmo, 1995.
48. Buffone GL, Frost A, Samo T, Demmler GJ, Cagle PT, Lawrence EC. The diagnosis of CMV pneumonitis in lung and heart/lung transplant patients by PCR compared with traditional laboratory criteria. Transplantation 1993; 56: 342-347.
49. Zipeto D, Revello MG, Silini E, Perea M, Percivalle E, Zavattoni M et al. Development and clinical significance of a diagnostic assay based on the polymerase chain reaction for detection of human cytomegalovirus DNA in blood samples from immunocompromised patients. J Clin Microbiol 1992; 30: 527-530.
50. Delgado R, Lumbreras C, Alba C, Pedraza MA, Otero JR, Gómez R et al. Low predictive value of polymerase chain reaction for diagnosis of cytomegalovirus disease in liver transplant recipients. J Clin Microbiol 1992; 30: 1.876-1.878.
51. Weber B, Nester U, Rabenau EH, Braner J, Birkenbach A, Scheuermann EH et al. Low correlation of human cytomegalovirus DNA amplification by polymerase chain reaction with cytomegalovirus disease in organ transplant recipients. J Med Virol 1994; 43: 187-193.
52. Brytting M, Mousavi-Jazi M, Bostrom L, Larsson M, Lunderberg J, Ljungman P et al. Cytomegalovirus DNA in peripheral blood leukocytes and plasma from bone marrow transplant recipients. Transplantation 1995; 60: 961-965.
53. Wolf DG, Spector SA. Early diagnosis of human cytomegalovirus disease in transplant recipients by DNA amplification in plasma. Transplantation 1993; 56: 330-334.
54. Zipeto D, Morris S, Hong C, Dowling A, Wolitz R, Merigan TC et al. Human cytomegalovirus (CMV) DNA in plasma reflects quantity of CMV DNA present in leukocytes. J Clin Microbiol 1995; 2.607-2.611.
55. Gerna G, Furione M, Baldanti F, Percivalle E, Colomi P, Locatelli F. Quantitation of human cytomegalovirus DNA in bone marrow transplant recipients. Br J Haematol 1995; 91: 674-683.
56. Drouet E, Colimon R, Michelson S, Fourcade N, Nieleau A, Ducerf C et al. Monitoring levels of human cytomegalovirus DNA in blood after liver transplantation. J Clin Microbiol 1995; 33: 389-391.
57. Patel R, Smith TF, Espy M, Wiesner RH, Krom RAF, Portela D et al. Detection of cytomegalovirus DNA in sera of liver transplant recipients. J Clin Microbiol 1994; 32: 1.431-1.434.
58. Yuen KY, Lo SKF, Chiu EKW, Woing SSY, Lau YL, Liang R et al. Monitoring of leukocyte cytomegalovirus DNA in bone marrow transplant recipients by nested PCR. J Clin Microbiol 1995; 33: 2.530-2.534.
59. Patel R, Smith TF, Espy M, Portela D, Wiesner RH, Krom RA, Paya CV. A prospective comparison of molecular diagnostic techniques for the early detection of cytomegalovirus in liver transplant recipients. J Infect Dis 1995; 171: 1.010-1.014.
60. Meyer-Konig U, Serr A, van Laer D, Kirste G, Wolff C, Haller O et al. Human cytomegalovirus immediate early and late transcripts in peripheral blood leukocytes: diagnostic value in renal transplant recipients. J Infect Dis 1995; 171: 705-709.
61. Velzing J, Rothbarth PH, Kroes ACM, Quint WGV. Detection of cytomegalovirus mRNA and DNA encoding the immediate early gene in peripheral blood leukocytes from immunocompromised patients. J Med Virol 1994; 42: 164-169.
62. Randhawa PS, Mañez R, Frye B, Erlich GD. Circulating immediate-early mRNA in patients with cytomegalovirus infections after solid organ transplantation. J Infect Dis 1994; 170: 1.264-1.267.
63. Bitsch A, Kirchner H, Dupke R, Bein G. Cytomegalovirus transcripts in peripheral blood leukocytes of actively infected patients detected by reverse transcription-polymerase chain reaction. J Infect Dis 1993; 167: 740-743.