A PCR assay to identify and distinguish single juveniles of Meloidogyne hapla and M. chitwoodi

Journal of Nematology

Volumn 29, Issue 1, 1997, Pages 9-15

  Williamson, V M ^b   Caswell Chen, E P ^b   Westerdahl, B B ^b   Wu, F F ^b   Caryl, G ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b NONE

Author keywords

Diagnostic; DNA; Meloidogyne chitwoodi; Meloidogyne hapla; PCR; Root knot nematode; SCAR; STS

Indexed keywords

MELOIDOGYNE CHITWOODI; MELOIDOGYNE HAPLA;

EID: 0031006834     PISSN: 0022300X     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (19)

1. Baum, T. J., P. M. Gresshoff, S. A. Lewis, and R. A. Dean. 1994. Characterization and phylogenetic analysis of four root-knot nematode species using DNA amplification fingerprinting and automated polyacrylamide gel clectrophoresis. Molecular Plant-Microbe Interactions 7:39-47.
2. Brown, C. R., H. Mojtahedi, and G. S. Santo. 1989. Comparison of reproductive efficiency of Meloidogyne chitwoodi on Solanum bulbocastanum in soil and in vitro tests. Plant Disease 73:957-959.
3. Castagnone-Sereno, P., C. Piotte, J. Cijthof, P. Abad, E. Wajnberg, F. Vanlerberghemasutti, M. Bongiovanni, and A. Dalmasso. 1993. Phylogenetic relationships between amphimictic and parthenogenetic nematodes of the genus Meloidogyne as inferred from repetitive DNA analysis. Heredity 70:195-204.
4. Caswell-Chen, E. P., V. M. Williamson, and F. F. Wu. 1992. Random amplified polymorphic DNA analysis of Heterodera cruciferae and H. schachtii populations. Journal of Nematology 24:343-351.
5. Cenis, J. L. 1993. Identification of four major Meloidogyne spp. by random amplified polymorphic DNA (RAPD-PCR). Phytopathology 83:76-80.
6. Cenis, J. L., C. H. Opperman, and A. C. Triantaphyllou. 1992. Cytogenetic, enzymatic, and restriction fragment length polymorphism variation of Meloidogyne spp. from Spain. Phytopathology 82:527-531.
7. Cui, X., H. Li, T. M. Goradia, K. Lange, H. H. Kazazian, D. Galas, and N. Arnheim. 1989. Single-sperm typing-determination of genetic distance between the Gγ-globin and parathyroid hormone loci by using the polymerase chain reaction and allele-specific oligomers. Proceedings of the National Academy of Science USA 86:9389-9393.
8. Curran, J., M. A. McClure, and J. M. Webster. 1980. Genotypic differentiation of Meloidogyne populations by detection of restriction fragment length difference in total DNA. Journal of Nematology 18:83-86.
9. Eisenback, J. D., and H. H. Triantaphyllou. 1991. Root-knot nematodes: Meloidogyne species and races. Pp. 191-274 in W. R. Nickle, ed. Manual of Agricultural Nematology. New York: Marcel Dekker.
10. Esbenshade, P. R., and A. C. Triantaphyllou. 1990. Isozyme phenotypes for the identification of Meloidogyne species. Journal of Nematology 22:10-15.
11. Hyman, B. C., J. J. Peloquin, and E. G. Platzer. 1990. Optimization of mitochondrial DNA-based hybridization assays to diagnostics in soil. Journal of Nematology 22:273-278.
12. Jenkins, W. R. 1964. A rapid centrifugal-flotation technique for extracting nematodes from soil. Plant Disease Reporter 48:692.
13. Paran, I., and R. W. Michelmore. 1993. Development of reliable PCR-based markers linked to downy mildew resistance genes in lettuce. Theoretical and Applied Genetics 85:985-993.
14. Peloquin, J. J., D. M. Bird, I. Kaloshian, and W. C. Matthews. 1993. Isolates of Meloidogyne hapla with distinct mitochondrial genomes. Journal of Nematology 25:239-243.
15. Pinkerton, J., H. Mojtahedi, and G. S. Santo. 1987. Reproductive efficiency of Pacific Northwest populations of Meloidogyne chitwoodi on alfalfa. Plant Disease 71:345-348.
16. Powers, T. O., and T. S. Harris. 1993. A polymerase chain reaction method for identification of five major Meloidogyne species. Journal of Nematology 25:1-6.
17. Williams, J. G. K., A. Rafalski, and S. V. Tingy. 1993. Genetic analysis using RAPD markers. Methods in Enzymology 218:704-740.
18. Williamson, V. M., J-Y Ho, F. F. Wu, N. Miller, and I. Kaloshian. 1994. A PCR-based marker tightly linked to the nematode resistance gene, Mi, in tomato. Theoretical and Applied Genetics 87:757-763.
19. Zijlstra, C., A. E. M. Lever, B. J. Unek, and C. H. Vansilfhout. 1995. Differences between ITS regions of isolates of root-knot nematodes Meloidogyne hapla and M. chitwoodi. Phytopathology 85:1231-1237.