Neurons are generated in confluent astroglial cultures of rat neonatal neocrotex

Neuroscience

Volumn 78, Issue 4, 1997, Pages 957-966

  Hildebrand, B ^a   Olenik, C ^a   Meyer, D K ^a  

^a UNIVERSITY OF FREIBURG   (Germany)

Author keywords

astroglial cultures; neocortex; neurogenesis

Indexed keywords

BROXURIDINE; LIPOPOLYSACCHARIDE;

ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; CELL CULTURE; CELL DENSITY; DNA SYNTHESIS; MACROGLIA; NEOCORTEX; NERVE CELL DEGENERATION; NEWBORN; NONHUMAN; PRIORITY JOURNAL; RAT; RNA ANALYSIS;

ANIMALS; ANIMALS, NEWBORN; ASTROCYTES; BROMODEOXYURIDINE; CATTLE; CELL DIVISION; CELLS, CULTURED; CEREBRAL CORTEX; CULTURE MEDIA; IMMUNOHISTOCHEMISTRY; NEURONS; RATS;

EID: 0031006282     PISSN: 03064522     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0306-4522(96)00676-8     Document Type: Article

References (40)

1. Anchan R. M., Reh T. A., Angello J., Balliet A. and Walker M. (1991) EGF and TGF-α stimulate retinal neuroepithelial cell proliferation in vitro. Neuron 6, 923-936.
2. Angevine J. B. and Sidman R. L. (1961) Autoradiographic study of cell migration during histogenesis of cerebral cortex in the mouse. Nature 192, 766-768.
3. Bayer S. A., Altman J., Russo R. J., Dai X. and Simmons J. A. (1991) Cell migration in the rat embryonic neocortex. J. comp. Neurol. 307, 499-516.
4. Berry M., Rogers A. W. and Eayrs J. T. (1964) Pattern of cell migration during cortical histogenesis. Nature 203, 591-593.
5. Booher J. and Sensenbrenner M. (1972) Growth and cultivation of dissociated neurons and glial cells from embryonic chick, rat and human brain in flask cultures. Neurobiology 2, 97-105.
6. Bottenstein J. E., Skaper S. D., Varon S. S. and Sato G. H. (1980) Selective survival of neurons from chick embryo sensory ganglionic dissociates utilizing serum-free supplemented medium. Expl Cell Res. 125, 183-190.
7. Breen K. C., Robinson P. A., Wion D. and Anderton B. H. (1988) Partial sequence of the rat heavy neurofilament polypetide (NF-H). Identification of putative phophorylation sites. Fedn Eur. biochem. Socs Lett. 241, 213-218.
8. Craig C. G., Tropepe V., Morshead C. M., Reynolds B. A., Weiss S. and van der Koy D. (1996) In vivo growth factor expansion of endogenous subependymal neuronal precursor cell populations in the adult mouse brain. J. Neurosci. 16, 2649-2658.
9. Dahlstrand J., Lardelli M. and Lendahl U. (1995) Nestin mRNA expression correlates with the central nervous system progenitor cell state in many, but not all, regions of developing central nervous system. Devl Brain Res. 84, 109-129.
10. Davis A. A. and Temple S. (1994) A self-renewing multipotential stem cell in embryonic rat cerebral cortex. Nature 372, 263-266.
11. Gage F. H., Ray J. and Fisher L. J. (1995) Isolation, characterization, and use of stem cells from the CNS. A. Rev. Neurosci. 18, 159-192.
12. Gebicke-Härter P. J., Bauer J., Schobert A. and Northof H. (1989) Lipopolysaccharide-free conditions in primary astrocyte cultures allow growth and isolation of microglial cells. J. Neurosci. 9, 183-194.
13. Gensburger C., Labourdette G. and Sensenbrenner M. (1987) Brain basic fibroblast growth factor stimulates the proliferation of rat neuronal precursor cells in vitro. Fedn Eur. biochem. Socs Lett. 217, 1-5.
14. Ghosh A. and Greenberg M. E. (1995) Distinct roles for bFGF and NT-3 in the regulation of cortical neurogenesis. Neuron 15, 89-103.
15. Gritti A., Parati E. A., Cova L., Frolichsthal P., Galli R., Wanke E., Faravelli L., Morasutti D. J., Roisen F., Nickel D. D. and Vescovi A. L. (1996) Multipotential stem cells from the adult mouse brain proliferate and self-renew in response to basic fibroblast growth factor. J. Neurosci. 16, 1091-1100.
16. Höltke H.-J. and Kessler C. (1990) Non-radioactive labeling of RNA transcripts in vitro with the hapten digoxigenin (DIG); hybridization and ELISA-based detection. Nucl. Acid Res. 18, 5843-5851.
17. Kaplan M. S. (1981) Neurogenesis in the 3-month-old rat visual cortex. J. comp. Neurol. 195, 323-338.
18. Kilpatrick T. J. and Bartlett P. F. (1993) Cloning and growth of multipotential neural precursors: requirements for proliferation and differentiation. Neuron 10, 255-265.
19. Levison S. W. and Goldman J. E. (1993) Both oligodendrocytes and astrocytes develop from progenitors in the subventricular zone of postnatal rat forebrain. Neuron 10, 201-212.
20. Lieberburg I., Spinner N., Snyder S., Anderson J., Goldgaber D., Smulowitz M., Carroll Z., Emanuel B., Breitner J. and Rubin L. (1989) Cloning of a cDNA encoding the rat high molecular weight neurofilament peptide (NF-H): development and tissue expression in the rat, mapping of its human homologue to chromosomes 1 and 22. Proc. natn. Acad. Sci. U.S.A. 86, 2463-2467.
21. Lillien L. and Cepko C. (1992) Control of proliferation in the retina: temporal changes in responsiveness to FGF and TGF-α. Development 115, 253-266.
22. Lois C. and Alvarez-Buylla A. (1993) Proliferating subventricular zone cells in the adult mammalian forebrain can differentiate into neurons and glia. Proc. natn. Acad. Sci. U.S.A. 90, 2074-2077.
23. LoTurco J. and Kriegstein A. R. (1991) Clusters of coupled neuroblasts in embryonic neocortex. Science 252, 563-566.
24. McCarthy K. D. and de Vellis J. (1977) Age dependent changes in neuronal and glial markers in cultures from rat cerebral cortex. Trans. Am. Soc. Neurochem. 8, 88.
25. McCarthy K. D. and de Vellis J. (1980) Preparation of separate astroglial and oligodendroglial cell cultures from rat cerebral tissue. J. Cell Biol 85, 890-902.
26. Morrison R. S., Sharma A., de Vellis J. and Bradshaw R. A. (1986) Basic fibroblast growth factor supports the survival of cerebral cortical neurons in primary culture. Proc. natn. Acad. Sci. U.S.A. 83, 7537-7541.
27. Murphy M., Drago J. and Bartlett P. F. (1990) Fibroblast growth factor stimulates the proliferation and differentiation of neural precursor cells in vitro. J. Neurosci. Res. 25, 463-475.
28. Pixley S. K. (1992) CNS glial cells support in vitro survival, division, and differentiation of dissociated olfactory neuronal progenitor cells. Neuron 8, 1191-1204.
29. Ray J. and Gage F. H. (1994) Spinal cord neuroblasts proliferate in response to basic fibroblasts growth factor. J. Neurosci. 14, 3548-3664.
30. Ray J., Peterson D. A., Schinstine M. and Gage F. H. (1993) Proliferation, differentiation, and long-term culture of primary hippocampal neurons. Proc. natn. Acad. Sci. U.S.A. 90, 3602-3606.
31. Reynolds B. A. and Weiss S. (1992) Generation of neurons and astrocytes from isolated cells of the adult mammalian central nervous system. Science 255, 1707-1710.
32. Richards L. J., Kilpatrick T. J. and Bartlett P. F. (1992) De novo generation of neuronal cells from the adult mouse brain. Proc. natn Acad. Sci. U.S.A. 89, 8591-8595.
33. Rudge J. S. (1993) Astrocyte-derived neurotrophic factors. In Astrocytes: Pharmacology and Function (ed. Murphy S.), pp. 267-305. Academic, New York.
34. Sensenbrenner M. (1977) Dissociated brain cells in primary cultures. In Cell, Tissue and Organ Cultures in Neurobiology (eds Fedoroff S. and Hertz L.), pp. 191-213. Academic, New York.
35. Sivron T., Eitan S., Schreyer D. J. and Schwartz M. (1993) Astrocytes play a major role in the control of neuronal proliferation in vitro. Brain Res. 629, 199-208.
36. Takei N., Tsukui H. and Hatanaka H. (1989) Intracellular storage and evoked release of acetylcholine from postnatal rat basal forebrain cholinergic neurons in culture with nerve growth factor. J. Neurochem. 53, 1405-1410.
37. Temple S. and Davis A. A. (1994) Isolated rat cortical progenitor cells are maintained in division in vitro by membrane-associated factors. Development 120, 999-1008.
38. Vescovi A. L., Reynolds B. A., Fraser D. D. and Weiss S. (1993) bFGF regulates the proliferative fate of unipotent (neuronal) and bipotent (neuronal/astroglial) EGF-generated CNS progenitors. Neuron 11, 951-966.
39. Weiss S., Reynolds B. A., Vescovi A. L., Morshead C., Craig C. G. and van der Koy D. (1996) Is there a neural stem cell in the mammalian forebrain? Trends Neurosci. 19, 387-393.
40. Williams B. P., Read J. and Price J. (1991) The generation of neurons and oligodendrocytes from a common precursor cell. Neuron 7, 685-693.