Cloning, heterologous expression and antigenicity of a schistosome cercarial protease

Parasitology

Volumn 114, Issue 5, 1997, Pages 447-453

  Price, H P ^a   Doenhoff, M J ^a   Sayers, J R ^b,c  

^a BANGOR UNIVERSITY   (United Kingdom)

^b UNIVERSITY OF SHEFFIELD   (United Kingdom)

^c UNIVERSITY OF SHEFFIELD   (United Kingdom)

Author keywords

cercarial elastase; expression; recombinant fusion proteins; Schistosoma mansoni

Indexed keywords

HYBRID PROTEIN; PROTEINASE;

ARTICLE; CERCARIA; CONTROLLED STUDY; DNA SEQUENCE; GENE EXPRESSION; MOLECULAR CLONING; NONHUMAN; PLASMID; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SCHISTOSOMA;

ANIMALS; ANTIBODIES, HELMINTH; ANTIBODY SPECIFICITY; ANTIGENS, HELMINTH; BASE SEQUENCE; CLONING, MOLECULAR; CONSENSUS SEQUENCE; ENDOPEPTIDASES; ESCHERICHIA COLI; EXONS; GENE EXPRESSION; GENES, HELMINTH; MALE; MOLECULAR SEQUENCE DATA; RECOMBINANT FUSION PROTEINS; SCHISTOSOMA MANSONI; SEQUENCE ANALYSIS, DNA;

EID: 0031005855     PISSN: 00311820     EISSN: None     Source Type: Journal    
DOI: 10.1017/S0031182096008657     Document Type: Article

References (33)

1. BABA, E. H., HOMEWOOD, C. A., GAZINELLI, G. & ATKINSON, E. M. (1977). Comparison of proteolytic enzymes from cercarial extract and secretion. Comparative Biochemistry and Physiology B-Biochemistry and Molecular Biology 57B, 55-57.
2. BICKLE, Q. D. & DOENHOFF, M. J. (1987). Comparison of the live vaccine potential of different geographic isolates of Schistosoma mansoni. Journal of Helminthology 61, 191-195.
3. BURNETTE, W. N. (1981). Western blotting electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein-A. Analytical Biochemistry 112, 195-205.
4. CAMPBELL, D. L., FRAPPAOLO, P. J. F., STIREWALT, M. A. & DRESDEN, M. H. (1976). Schistosoma mansoni: partial characterization of enzyme(s) secreted from the preacetabular glands of cercariae. Experimental Parasitology 40, 33-40.
5. CHARRIER-FERRARA, S., DJABALI, M. & GOUDOT-CROZEL, V. (1991). Schistosoma mansoni a simple method for the extraction of DNA from single worms for PCR amplification. Experimental Parasitology 73, 384.
6. CRAIG, S. P., MURALIDHAR, M. G., McKERROW, J. H. & WANG, C. C. (1989). Evidence for a class of very small introns in the gene for hypoxanthine-guanine phosphoribosyl transferase in Schistosoma mansoni. Nucleic Acids Research 17, 1635-1647.
7. CRAIK, C. S., RUTTER, W. J. & FLETTERICK, R. (1983). Splice junctions: association with variation in protein structure. Science 220, 1125-1129.
8. CURTIS, R. H. C. (1988). Characterisation of some Schistosoma mansoni hydrolases and other surface antigens. Ph.D. thesis, University of London, UK.
9. DOENHOFF, M. J., CURTIS, R. H., NGAIZA, J. & MODHA, J. (1990). Proteases in the schistosome life cycle: a paradigm for tumour metastasis. Cancer and Metastasis Reviews 9, 381-392.
10. GUAN, K. L. & DIXON, J. E. (1991). Eukaryotic proteins expressed in Escherichia coli: an improved thrombin cleavage and purification procedure of fusion proteins with glutathione S-transferase. Analytical Biochemistry 192, 262-267.
11. KRAUSE, M. & HIRSH, D. (1987). A trans-spliced leader sequence on actin messenger-RNA in C. elegans. Cell 49, 753-761.
12. KUMAR, S. & PRITCHARD, D. I. (1992). The partial characterization of proteases present in the excretory/secretory products and exsheathing fluid of the infective (L3) larva of Necator amertcanus. International Journal for Parasitology 22, 563-572.
13. LAEMMLI, U. K. & FAVRE, M. (1973). Maturation of the head of bacteriophage T4. I. DNA packaging events. Journal of Molecular Biology 80, 575-599.
14. LANDSPERGER, W. J., STIREWALT, M. A. & DRESDEN, M. H. (1982). Purification and properties of a proteolytic enzyme from the cercariae of the human trematode parasite Schistosoma mansoni. The Biochemical Journal 201, 137-144.
15. LEWERT, R. M. & LEE, C. L. (1956). Quantitative studies of the collagenase-like enzymes of cercariae of Schistosoma mansoni and the larvae of Strongyloides ratti. Journal of Infectious Diseases 99, 1-14.
16. LORENS, J. B. (1991). Rapid and reliable cloning of PCR products. PCR Methods and Applications 1, 140-141.
17. McKERROW, J. H. & DOENHOFF, M. J. (1988). Schistosome proteases. Parasitology Today 4, 334-340.
18. McKERROW, J. H., JONES, P., SAGE, H. & PINO-HEISS, S. (1985a). Proteinases from invasive larvae of the trematode parasite Schistosoma mansoni degrade connective-tissue and basement-membrane macromolecules. The Biochemical Journal 231, 47-51.
19. McKERROW, J. H., KEENE, W. E., JEONG, K. H. & WERB, Z. (1983). Degradation of extracellular matrix by larvae of Schistosoma mansoni. I. Degradation by cercariae as a model for initial parasite invasion of host. Laboratory Investigation 49, 195-200.
20. McKERROW, J. H., NEWPORT, G. & FISHELSON, Z. (1991). Minireview. Recent insights into the structure and function of a larval proteinase involved in host infection by a multicellular parasite. Proceedings of the Society for Experimental Biology and Medicine 197, 119-124.
21. McKERROW, J. H., PINO-HEISS, S., LINDQUIST, R. & WERB, Z. (1985b). Purification and characterization of an elastolytic proteinase secreted by cercariae of Schistosoma mansoni. Journal of Biological Chemistry 260, 3703-3707.
22. MULLER, N. & FELLEISEN, R. (1995). Bacterial expression systems as tools for the production of immunodiagnostic parasite antigens. Parasitology Today 11, 476-480.
23. MULLIS, K. B. & FALOONA, F. A. (1987). Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction. Methods in Enzymology 155, 335-350.
24. NEWPORT, G. R., McKERROW, J. H., HEDSTROM, R., PETITT, M., McGARRIGLE, L., BARR, P. J. & AGABIAN, N. (1988). Cloning of the proteinase that facilitates infection by schistosome parasites. Journal of Biological Chemistry 263, 13179-13184.
25. OETTINGER, H. F., PASQUELINE, R. & BERNFIELD, M. (1992). Recombinant peptides as immunogens a comparison of protocols for antisera production using the pGEX system. Biotechniques 12, 544-549.
26. OHSHIMA, Y. & GOTOH, Y. (1987). Signals for the selection of a splice site in pre-mRNA. Computer analysis of splice junction sequences and like sequences. Journal of Molecular Biology 195, 247-259.
27. PIERROT, C., CAPRON, A. & KHALIFE, J. (1995). Cloning and characterization of two genes encoding Schistosoma mansoni elastase. Molecular and Biochemical Parasitology 75, 113-117.
28. POTTER, H. (1988). Electroporation in biology - methods, applications and instrumentation. Analytical Biochemistry 174, 361-373.
29. PRICE, H. P., DOENHOFF, M. J. & SAYERS, J. R. (1996). Expression and antigenicity of a schistosome cercarial protease. Transactions of the Royal Society of Tropical Medicine and Hygiene 90, 7.
30. SANGER, F., NICKLEN, S. & COULSON, A. R. (1977). DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences, USA 74, 5463-5467.
31. SIMONS, P. C. & VAN DER JAGT, D. L. (1981). Purification of glutathione S-transferases by glutathione-affinity chromatography. Methods in Enzymology 77, 235-237.
32. SMITH, D. B. & JOHNSON, K. S. (1988). Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferases. Gene 67, 31-40.
33. STIREWALT, M. A. & FREGEAU, W. A. (1966). An invasive enzyme system present in cercariae but absent in schistosomules of Schistosoma mansoni. Experimental Parasitology 19, 206-215.