Effectiveness of protein A for antibody immobilization for a fiber optic biosensor

Biosensors and Bioelectronics

Volumn 12, Issue 4, 1997, Pages 329-336

  Anderson, George P ^a   Jacoby, Meagan A ^a   Ligler, Frances S ^a   King, Keeley D ^b  

^a NAVAL RESEARCH LABORATORY   (United States)

^b SCIENCE APPLICATIONS INTERNATIONAL CORPORATION   (United States)

Author keywords

antibodies; fiber optic biosensor; protein A; regeneration

Indexed keywords

ANTIBODIES; ANTIGEN-ANTIBODY REACTIONS; ANTIGENS; BIOASSAY; CELL IMMOBILIZATION; FIBER OPTIC SENSORS; IMMUNOLOGY; PH EFFECTS; PROTEINS;

ANTIBODY IMMOBILIZATION; ASSAY REGENERATION; FIBER OPTIC BIOSENSOR; FLUOROIMMUNOASSAYS;

BIOSENSORS;

BINDING PROTEIN; IMMUNOGLOBULIN G; IMMUNOGLOBULIN G ANTIBODY; PROTEIN A;

ANTIGEN ANTIBODY COMPLEX; ARTICLE; BIOSENSOR; FIBEROSCOPE; IMMOBILIZATION;

ANTIGEN-ANTIBODY REACTIONS; BIOSENSING TECHNIQUES; FIBER OPTICS; FLUOROIMMUNOASSAY; IMMUNOGLOBULIN G; STAPHYLOCOCCAL PROTEIN A;

CAPRA HIRCUS; ORYCTOLAGUS CUNICULUS; YERSINIA PESTIS;

EID: 0031003589     PISSN: 09565663     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0956-5663(96)00074-7     Document Type: Article

References (16)

1. Anderson, G.P., Golden, J.P. & Ligler, F.S. (1993). A fiber optic biosensor: combination tapered fibers designed for improved signal acquisition. Biosensors & Bioelectronics, 8, 249-256.
2. Bennett, L.G. & Tornabene, T.G. (1974). Characterization of the antigenic subunits of the envelope protein of Yersinia pestis. J. Bacteriol., 117, 48-55.
3. Bhatia, S.K., Shriver-Lake, L.C., Prior, K.J., Georger, J.H., Calvert, J.M., Bredehorst, R. & Ligler, F.S. (1989). Use of thiol-terminal silanes and heterobifunctional cross-linkers for immobilization of antibodies on silica surfaces. Anal. Biochem., 178. 408-413.
4. Cao, L.K., Anderson, G.P., Ligler, F.S. & Ezzell, J. (1994). Detection of Yersinia pestis fraction 1 antigen with a fiber optic biosensor. J. Clin. Microbiol., 33, 336-341.
5. Fulton, S.P. (1989). Antibody purification techniques. W.R. Grace & Co. Publ. No. 8685M589, pp. 46-47.
6. Golden, J.P., Shiver-Lake, L.C., Anderson, G.P., Thompson, R.B. & Ligler, F.S. (1992). Fluorometer and tapered fiber optic probes for sensing in the evanescent wave. Optical Eng., 31, 1458-1462.
7. Ligler, F.S., Golden, J.P., Shiver-Lake, L.C., Ogert, R.A., Wijesuria, D. & Anderson, O.P. (1993). Fiber-optic biosensors for the detection of hazardous materials. Immunomethods, 3, 122-127.
8. Ngai, P.K.M., Ackermann, F., Wendt, H., Savoca, R. & Bosshard, H.R. (1993). Protein-A antibody-capture ELISA (Pace)-an ELISA format to avoid denaturation of surface-adsorbed antigens. J. Immunol. Meth., 158, 267-276.
9. Owaku, K., Goto, M., Ikariyama, Y. & Aizawa, M. (1995). Protein-A Langmuir-Blodgett-film for antibody immobilization and its use in optical immunosensing. Anal. Chem., 67, 1613-1616.
10. Palmer, D.A., Evans, M., Miller, J.N. & French, M.T. (1994). Rapid fluorescence flow-injection immunoassay using a novel perfusion chromatographic material. Analyst, 119, 943-947.
11. Ren, X.Z. & Miller, J.N. (1994). Flow-injection fluoroimmunoassay for human transferrin using a protein-A immunoreactor. Anal. Lett., 27(6), 1067-1074.
12. Shiver-Lake, L.C., Breslin, K.A., Charles, P.T., Conrad, D.W., Golden, J.P. & Ligler, F.S. (1995). Detection of TNT in water using an evanescent wave fiber-optic biosensor. Anal. Chem., 34, 2431-2435.
13. Tempelman, L.A., Golden, J.G., Anderson, G.P. & Ligler, F.S. (1994). Use of cyanine dyes with evanescent wave fiber optic biosensors. SPIE, 2293, 139-148.
14. Tempelman, L.A., King, K.D., Anderson, G.P. & Ligler, F.S. (1995). Quantitating staphylococcal enterotoxin b in diverse media using a portable fiber-optic biosensor. Anal. Biochem., 233, 50-57.
15. Widjojoatmodjo, M.N., Fluit, A.C., Torensma, R. & Verhoef, J. (1993). Comparison of immunomagnetic beads coated with protein-A, protein-G, or goat anti-mouse immunoblobulins-applications in enzymes immunoassays and immunomagnetic separations. J. Immunol. Meth., 165, 11-19.
16. Wijesuriya, D., Breslin, K., Anderson, G.P., Shiver-Lake, L.C. & Ligler, F.S. (1994). Regeneration of immobilized antibodies on fiber optic probes. Biosensors & Bioelectronics, 9, 585-592.