Removal of osteoclast bone resorption products by transcytosis

Science

Volumn 276, Issue 5310, 1997, Pages 270-273

  Salo, Jari ^a   Lehenkari, Petri ^a   Mulari, Mika ^a   Metsikkö, Kalervo ^a   Väänänen, H Kalervo ^a,b  

^a UNIVERSITY OF OULU   (Finland)

^b UPPSALA UNIVERSITY   (Sweden)

Author keywords

[No Author keywords available]

Indexed keywords

ANIMAL CELL; ARTICLE; CELL MEMBRANE TRANSPORT; CONTROLLED STUDY; ENDOCYTOSIS; NONHUMAN; OSTEOCLAST; OSTEOLYSIS; PRIORITY JOURNAL; RAT; TRANSCYTOSIS;

ACTINS; ANIMALS; BIOLOGICAL TRANSPORT; BIOTIN; BONE MATRIX; BONE RESORPTION; CATTLE; CELL MEMBRANE; CELL POLARITY; CELLS, CULTURED; ENDOCYTOSIS; EXTRACELLULAR SPACE; MICROSCOPY, CONFOCAL; MICROSCOPY, ELECTRON; MINERALS; ORGANELLES; OSTEOCALCIN; OSTEOCLASTS; RATS; TETRACYCLINE;

ANIMALIA;

EID: 0031002026     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.276.5310.270     Document Type: Article

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23. Species-specific polyclonal antibodies (Biogenesis, Poole, England) that recognize bovine but not rat osteocalcin were used (dilution, 1:100, for 60 min at 37°C). Eight independent experiments were performed. Rat osteoclasts were cultured as described for Fig. 1.
24. LF-BON-I antibodies to purified bovine bone osteonectin were a gift from L. W. Fisher, NIH, Bethesda, MD.
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29. 2 (0.6 mg/ml) reaction was carried out. Cells were fixed in 2.5% glutaraldehyde for 90 min at 4°C and osmicated. Bone slices were then decalcified in sodium cacodylate-buffered EDTA (4.13 mg/ml) containing 0.1% glutaraldehyde for 3 days at 4°C, dehydrated, and embedded in Epon LX-112. Thin sections (100 nm) were stained with lead citrate and observed with a transmission electron microscope (Philips 410, at 60 kV). Twenty individual osteoclasts from three independent cultures were analyzed.
30. Supported by the Finnish Academy of Sciences and by Astra Hässle AB, Sweden. We thank S. Seinijoki and A. Haimakainen for technical assistance.