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Volumn 276, Issue 5310, 1997, Pages 256-259

Phylogenetic analysis of glycolytic enzyme expression

Author keywords

[No Author keywords available]

Indexed keywords

GLYCOLYTIC ENZYME;

EID: 0031002025     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.276.5310.256     Document Type: Article
Times cited : (92)

References (38)
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    • note
    • The term "equilibrium enzymes" is used here to denote enzymes that catalyze reactions that proceed in either the forward or reverse direction depending on the relative concentrations of substrates and products. An alternative term is "intervening step enzyme."
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    • note
    • Abbreviations for glycolytic enzymes: hexokinase (HK, E.C. 2.7.1.1), phosphoglucoisomerase (PGI, E.C. 5.3.1.9), phosphofructokinase (PFK, E.C. 2.7.1.11), adlolase (ALD, E.C. 4.1.2.13), triose-phosphate isomerase (TPI, E.C. 5.3.1.1), glyceraldehyde-3-phosphate dehydrogenase (GAPDH, E.C. 1.2.1.12), phosphoglycerokinase (PGK, E.C. 2.7.2.3), phosphoqlyceromutase (PGM, E.C. 2.7.5.3), enolase (ENO, E.C. 4.2.1.11), pyruvate kinase (PYK, E.C. 2.7.1.40), lactate dehydrogenase (LDH, E.C. 1.1.1.27).
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    • Although the validity of either punctuational or gradual evolutionary models is unresolved, an assumption is required to statistically analyze these data. Both simulations and empirical studies suggest the phylogenetic methods used here are relatively insensitive to the assumption of punctuational models even when evolution is gradual [A. Purvis, J. L. Gittleman, H.-K. Luh, J. Theor. Biol. 167, 293 (1994); M. Westneat, Environ. Biol. Fishes 44, 263 (1995); J. L. Gittleman and H.-K. Luh, Eds., Phylogenetics and Ecology; Joint Symposium Between the Natural History Museum and the Linnean Society of London (Academic Press, London, 1994).
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    • Although the validity of either punctuational or gradual evolutionary models is unresolved, an assumption is required to statistically analyze these data. Both simulations and empirical studies suggest the phylogenetic methods used here are relatively insensitive to the assumption of punctuational models even when evolution is gradual [A. Purvis, J. L. Gittleman, H.-K. Luh, J. Theor. Biol. 167, 293 (1994); M. Westneat, Environ. Biol. Fishes 44, 263 (1995); J. L. Gittleman and H.-K. Luh, Eds., Phylogenetics and Ecology; Joint Symposium Between the Natural History Museum and the Linnean Society of London (Academic Press, London, 1994).
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    • note
    • This project was aided by discussions with T. Garland, L. McIntyre, M. Westneat, E. Wiley, and J. Segal and by critical readings of the manuscript by A. Gibbs, S. Hand, R. Huey, and three anonymous reviewers. For help locating and obtaining species, we thank K. Able, L. Barnett, G. Byerly, R. Kneib, L. McKelvey, S. McMann, A. Pierce, M. Thayer, and E. Wiley; M. Eberle, and E. H. Edwards of the NC Parks Department; L. Fuiman and his lab; and the Department of Marine Resources at the Marine Biological Laboratory (Woods Hole, MA). Supported by NSF grant IBN-9419781 (to D.L.C.) and the Hinds Fund and Sigma Xi (to V.A.P.). V.A.P. is a Howard Hughes Medical Institute Predoctoral Fellow.


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