The recovery of a B2 insertion in the lacI gene of a rat cell line containing a λ/LIZ shuttle vector

Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis

Volumn 375, Issue 1, 1997, Pages 79-82

  De Boer, Johan G ^a   Suri, Andrew ^a   Walsh, David ^a   Glickman, Barry W ^a  

^a UNIVERSITY OF VICTORIA   (Canada)

Author keywords

Big Blue; lacI transgene; Repeated sequence; Retroposon

Indexed keywords

ANIMAL CELL; ARTICLE; CELL LINE; FIBROBLAST; GENE DELETION; GENE INSERTION; MUTANT; NONHUMAN; PRIORITY JOURNAL; RAT; RETROPOSON; SHUTTLE VECTOR; TRANSGENE;

ANIMALS; ANIMALS, GENETICALLY MODIFIED; BACTERIAL PROTEINS; BACTERIOPHAGE LAMBDA; BASE SEQUENCE; CELL LINE; DNA TRANSPOSABLE ELEMENTS; ESCHERICHIA COLI PROTEINS; FIBROBLASTS; GENETIC VECTORS; MOLECULAR SEQUENCE DATA; MUTATION; POLYMERASE CHAIN REACTION; RATS; REPETITIVE SEQUENCES, NUCLEIC ACID; REPRESSOR PROTEINS; SEQUENCE ALIGNMENT; SEQUENCE HOMOLOGY, NUCLEIC ACID; VITAMIN K;

EID: 0031001897     PISSN: 00275107     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0027-5107(96)00255-2     Document Type: Article

References (15)

1. Wallace, M.R., Andersen, L.B., Saulino, A.M., Gregory, P.E., Glover, T.W. and Collins, F.S. (1991) A de novo Alu insertion results in neurofibromatosis type 1. Nature, 353, 864-866.
2. Janicic, N., Pausova, Z., Cole, D.E. and Hendy, G.N. (1995) Insertion of an Alu sequence in the Ca(2+)-sensing receptor gene in familial hypocalciuric hypercalcemia and neonatal severe hyperparathyroidism. Am. J. Hum. Genet., 56, 880-886.
3. Vidaud, D., Vidaud, M., Bahnak, B.R., Siguret, V., Gispert Sanchez, S., Laurian, Y., Meyer, D., Goossens, M. and Lavergne, J.M. (1993) Haemophilia B due to a de novo insertion of a human-specific Alu subfamily member within the coding region of the factor IX gene. Eur. J. Hum. Genet., 1, 30-36.
4. Rogers, J.H. (1985) The origin and evolution of retroposons. Inter. Rev. Cytol., 93, 187-279.
5. Maichele, A., Farwell, N.J. and Chamberlain, J.S. (1993) A B2 repeat insertion generates alternate structures of the mouse muscle gamma-phosphorylase kinase gene. Genomics, 16, 139-149.
6. Zheng, J.H., Natsuume-Sakai, S., Takahashi, M. and Nonaka, M. (1992) Insertion of the B2 sequence into intron 13 is the only defect of the H-2k C4 gene which causes low C4 production. Nucleic Acids Res., 20, 4975-4979.
7. Pattanakitsakul, S., Zheng, J.H., Natsuume-Sakai, S., Takahashi, M. and Nonaka, M. (1992) Aberrant splicing caused by the insertion of the B2 sequence into an intron of the complement C4 gene is the basis for low C4 production in H-2k mice. J. Biol. Chem., 267, 7814-7820.
8. Oberbaumer, I. (1992) Retroposons do jump: a B2 element recently integrated in an 18S rDNA gene. Nucleic Acids Res., 20, 671-677.
9. Wyborski, D., Malkhosyan, S., Moores, J., Perucho, M. and Short, J. (1995) Development of a rat cell line containing stably integrated copies of a lambda/lacI shuttle vector. Mutation Res., 334, 161-166.
10. Suri, A., De Boer, J.G., Kusser, W.C. and Glickman, B.W. (1996) A 3 milliTesla 60 Hz magnetic field is neither mutagenic nor co-mutagenic in the presence of menadione and MNU in a transgenic rat cell line. Mutation Res., 372, 23-31.
11. Erfle, H., Walsh, D., Holcroft, J., Hague, N., De Boer, J.G. and Glickman, B.W. (1996) An efficient laboratory protocol for the sequencing of large numbers of lad mutants recovered from Big Blue transgenic animals. Environ. Mol. Mutagen., 28, 393-396.
12. Den Dunnen, J.T. and Schoenmakers, J.G.G. (1987) Consensus sequences of the Rattus norvegicus B1- and B2 repeats. Nucleic Acids Res., 15, 2772.
13. Sawada, M., Sofuni, T. and Ishidate, Jr. (1991) Isolation of a menadione-resistant subclone from Chinese hamster lung (CHL) cells in culture. Mutation Res., 249, 7-17.
14. Nutter, L.M., Ngo, E.O., Fisher, G.R. and Gutierrez, P.L. (1992) DNA strand scission and free radical production in menadione treated cells. Correlation with cytotoxicity and role of NADPH quinone acceptor oxidoreductase. J. Biol. Chem., 267, 2474-2478.
15. Heng, H.H.Q., Tsui, L.-C. and Moens, P.B. (1994) Organization of heterologous DNA inserts on the mouse meiotic chromosome core. Chromosoma, 103, 401-407.