Mutation of the GKS motif of the RNA-dependent RNA polymerase from potato virus X disables or eliminates virus replication

Journal of General Virology

Volumn 78, Issue 6, 1997, Pages 1247-1251

  Davenport, Guy F ^a   Baulcombe, David C ^a  

^a SAINSBURY LABORATORY   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

ALANINE; ASPARAGINE; GLUTAMIC ACID; GLYCINE; LYSINE; MUTANT PROTEIN; RNA DIRECTED RNA POLYMERASE; SERINE; VIRUS ENZYME;

AMINO ACID SUBSTITUTION; ARTICLE; PLANT VIRUS; PRIORITY JOURNAL; RNA VIRUS; VIRUS INHIBITION; VIRUS MUTANT;

EID: 0031001430     PISSN: 00221317     EISSN: None     Source Type: Journal    
DOI: 10.1099/0022-1317-78-6-1247     Document Type: Article

References (27)

1. Baulcombe, D. C., Flavell, R. B., Boulton, R. E. & Jellis, G. J. (1984). The sensitivity and specificity of a rapid nucleic acid hybridization method for the detection of potato virus X in crude sap samples. Plant Pathology 33, 361-370.
2. Bordo, D. & Argos, P. (1991). Suggestions for 'safe' residue substitutions in site-directed mutagenesis. Journal of Molecular Biology 217, 721-729.
3. Bradley, M. K., Smith, T. F., Lathrop, R. H., Livingston, D. M. & Webster, T. A. (1987). Consensus topography in the ATP binding site of the simian virus 40 and polyomavirus large tumor antigens. Proceedings of the National Academy of Sciences, USA 84, 4026-4030.
4. Chapman, S. N., Kavanagh, T. A. & Baulcombe, D. C. (1992). Potato virus X as a vector for gene expression in plants. Plant Journal 2, 549-557.
5. Gorbalenya, A. E., Koonin, E. V., Donchenko, A. P. & Blinov, V. M. (1988). A conserved NTP-motif in putative helicases. Nature 333, 22.
6. Hanahan, D. (1983). Studies on transformation of Escherichia coli with plasmids. Journal of Molecular Biology 166, 557-580.
7. Hodgman, T. C. (1988). A new superfamily of replicative proteins. Nature 333, 22-23.
8. Inokuchi, Y. & Hirashima, A. (1987). Interference with viral infection by defective RNA replicase. Journal of Virology 61, 3946-3949.
9. Jablonski, S. A. & Morrow, C. D. (1995). Mutation of the aspartic acid residues of the GDD sequence motif of poliovirus RNA-dependent RNA polymerase results in enzymes with altered metal ion requirements for activity. Journal of Virology 69, 1532-1539.
10. Jablonski, S. A., Luo, M. & Morrow, C. (1991). Enzymatic activity of poliovirus RNA polymerase mutants with single amino acid changes in the conserved YGDD amino acid motif. Journal of Virology 65, 4565-4572.
11. Kavanagh, T. A., Goulden, M., Santa Cruz, S., Chapman, S. N., Barker, I. & Baulcombe, D. C. (1992). Molecular analysis of a resistance-breaking strain of potato virus X. Virology 189, 609-617.
12. Konola, J. T., Logan, K. M. & Knight, K. L. (1994). Functional characterisation of residues in the p-loop motif of RecA protein ATP binding site. Journal of Molecular Biology 237, 20-34.
13. Koonin, E. V. & Dolja, V. V. (1993). Evolution and taxonomy of positive-strand RNA viruses: implications of comparative analysis of amino acid sequences. Biochemistry and Molecular Biology 28, 375-430.
14. Kroner, P., Richards, D., Traynor, P. & Ahlquist, P. (1989). Defined mutations in a small region of the brome mosaic virus 2a gene cause diverse temperature-sensitive RNA replication phenotypes. Journal of Virology 63, 5302-5309.
15. Köhm, B. A., Goulden, M. G., Gilbert, J. E., Kavanagh, T. A. & Baulcombe, D. C. (1993). A potato virus X resistance gene mediates an induced, nonspecific resistance in protoplasts. Plant Cell 5, 913-920.
16. Logan, K. M. & Knight, K. L. (1993). Mutagenesis of the p-loop motif in the ATP binding site of the RecA protein from Escherichia coli. Journal of Molecular Biology 232, 1048-1059.
17. Longstaff, M., Brigneti, G., Boccard, F., Chapman, S. N. & Baulcombe, D. C. (1993). Extreme resistance to potato virus X infection in plants expressing a modified component of the putative viral replicase. EMBO Journal 12, 379-386.
18. Mirzayan, C. & Wimmer, E. (1992). Genetic analysis of NTP-binding motif in poliovirus polypeptide 2C. Virology 189, 547-555.
19. Momoeda, M., Wong, S., Kawase, M., Young, N. S. & Kajigaya, S. (1994). A putative nucleoside triphosphate-binding domain in the nonstructural protein of B19 parvovirus is required for cytotoxicity. Journal of Virology 68, 8443-8446.
20. Peters, S. A., Verver, J., Nollen, E. A. A., van Lent, J. W. M., Wellink, J. & Van Kammen, A. (1994). The NTP-binding motif in cowpea mosaic virus B polyprotein is essential for viral replication. Journal of General Virology 75, 3167-3176.
21. Sambrook, J., Fritsch, E. F. & Maniatis, T. (1989). Molecular Cloning: A laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
22. Saraste, M., Sibbald, P. R. & Wittinghofer, A. (1990). The P-loop - a common motif in ATP- and GTP-binding proteins. Trends In Biochemical Sciences 15, 430-434.
23. Story, R. M. & Steitz, T. A. (1992). Structure of the recA protein-ADP complex. Nature 355, 374-376.
24. Story, R. M., Bishop, D. K., Kleckner, N. & Steitz, T. A. (1993). Structural relationships of bacterial RecA proteins to recombination proteins from bacteriophage T4 and yeast. Science 259, 1892-1896.
25. Teterina, N. L., Kean, K. M., Gorbalenya, A. E., Agol, V. I. & Girard, M. (1992). Analysis of the functional significance of amino acid residues in the putative NTP-binding pattern of the poliovirus 2C protein. Journal of General Virology 73, 1977-1986.
26. Walker, J. E., Saraste, M., Runswick, M. J. & Gay, N. J. (1982). Distantly related sequences in the α- and β-subunits of ATP synthase, myosin, kinases and other ATP-requiring enzymes and a common nucleotide binding fold. EMBO Journal 1, 945-951.
27. Yao, Z., Jones, D. H. & Grose, C. (1992). Site-directed mutagenesis of herpesvirus glycoprotein phosphorylation sites by recombination polymerase chain reaction. PCR Methods and Applications 1, 205-207.