Mutational analysis of the hMLH1 gene using an automated two- dimensional DNA typing system

Human Mutation

Volumn 9, Issue 2, 1997, Pages 164-171

  Sasaki, Shin ^a   Tokino, Takashi ^a   Miyatsu, Takashi ^a   Muto, Tetsuichiro ^a   Nakamura, Yusuke ^a  

^a UNIVERSITY OF TOKYO   (Japan)

Author keywords

2 D DNA typing system; denaturing gradient gel electrophoresis (DGGE); hMLH1; mismatch repair genes; replication error; sporadic colorectal cancers

Indexed keywords

ARTICLE; BASE MISPAIRING; COLORECTAL CANCER; CONTROLLED STUDY; DNA DETERMINATION; ENDOMETRIUM CANCER; FAMILIAL CANCER; GENE; GENE MUTATION; HUMAN; HUMAN CELL; MAJOR CLINICAL STUDY; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL;

CARRIER PROTEINS; COLORECTAL NEOPLASMS; COLORECTAL NEOPLASMS, HEREDITARY NONPOLYPOSIS; DNA MUTATIONAL ANALYSIS; DNA REPAIR; DNA REPLICATION; ELECTROPHORESIS, POLYACRYLAMIDE GEL; EXONS; GENETIC SCREENING; HUMANS; MUTATION; NEOPLASM PROTEINS; NEOPLASMS, MULTIPLE PRIMARY; NUCLEAR PROTEINS; PHENOTYPE; POLYMERASE CHAIN REACTION; POLYMORPHISM, GENETIC;

EID: 0031000607     PISSN: 10597794     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1098-1004(1997)9:2<164::AID-HUMU9>3.0.CO;2-9     Document Type: Article

References (10)

1. Bronner CE, Baker SM, Morrison PT, Warren G, Smith LG, Lescoe MK, Kane M, Earabino C, Lipford J, Lindblom A, Tannergärd P, Bollag RJ, Godwin AR, Ward DC, Nordenskjøld M, Fishel R, Kolodner R, Liskay RM (1994) Mutation in the DNA mismatch repair gene homologue hMLH1 is associated with hereditary non- polyposis colon cancer. Nature 368:258-261.
2. Fodde R, Losekoot M (1994) Mutation detection by denaturing gradient gel electrophoresis (DGGE). Hum Mutat 3:83-94.
3. Ghanem N, Girodon E, Vidaud M, Martin J, Fanen P, Plassa F, Goossens M (1992) A comprehensive scanning method for rapid detection of β-Globin gene mutations and polymorphisms. Hum Mutat 1:229-239.
4. Lerman LS, Silverman K, Grinfield E (1986) Searching for gene defects by denaturing gradient gel electrophoresis. Cold Spring Harbor Symp Quant Biol 51:285-297.
5. Liu B, Nicolaides NC, Markowitz S, Willson JKV, Vogelstein B (1995) Mismatch repair gene defects in sporadic colorectal cancers with microsatellite instability. Nature Genet 9:48-55.
6. Mullaart E, de Vos GJ, te Meerman GJ, Uitterlinden AG, Vijg J (1993) Parallel genome analysis by two-dimensional DNA typing. Nature 365:469-471.
7. Myers RM, Fischer SO, Maniatis T, Lerman LS (1985a) Modification of the melting properties of duplex DNA by attachment of a GC-rich DNA sequence as determined by denaturing gradient gel electrophoresis. Nucleic Acids Res 13:3111-3129.
8. Myers RM, Fischer SG, Lerman LS, Maniatis T (1985b) Nearly all single base substitutions in DNA fragments joined to a GC-clamp can be detected by denaturing gradient gel electrophoresis. Nucleic Acids Res 13:3131-3145.
9. Uitterlinden AG, Vijg J (1994) Two-dimensional DNA typing: A parallel approach to genome analysis. Horwood, Chichester, U.K.
10. Vijg J, Wu Y, Uitterlinden AG, Mullaart E (1994) Two-dimensional DNA electrophoresis in mutation detection. Mutat Res 308:205-214.