-
1
-
-
76549204942
-
-
K. J. Bloch, W. W. Buchanan, M. J. Wohl, J. J. Bunim, Medicine 44, 187 (1965); E. K. L. Chan, J. C. Hamel, J. P. Buyon, E. M. Tan, J. Clin. Invest. 87, 68 (1991).
-
(1965)
Medicine
, vol.44
, pp. 187
-
-
Bloch, K.J.1
Buchanan, W.W.2
Wohl, M.J.3
Bunim, J.J.4
-
2
-
-
0026082028
-
-
K. J. Bloch, W. W. Buchanan, M. J. Wohl, J. J. Bunim, Medicine 44, 187 (1965); E. K. L. Chan, J. C. Hamel, J. P. Buyon, E. M. Tan, J. Clin. Invest. 87, 68 (1991).
-
(1991)
J. Clin. Invest.
, vol.87
, pp. 68
-
-
Chan, E.K.L.1
Hamel, J.C.2
Buyon, J.P.3
Tan, E.M.4
-
3
-
-
0022555525
-
-
R. I. Fox et al., Arthritis Rheum. 29, 577 (1986); A. K. Kruize, R. J. T. Smeenk, L. Kater, Immunol. Today 16, 557 (1995).
-
(1986)
Arthritis Rheum.
, vol.29
, pp. 577
-
-
Fox, R.I.1
-
4
-
-
0028875784
-
-
R. I. Fox et al., Arthritis Rheum. 29, 577 (1986); A. K. Kruize, R. J. T. Smeenk, L. Kater, Immunol. Today 16, 557 (1995).
-
(1995)
Immunol. Today
, vol.16
, pp. 557
-
-
Kruize, A.K.1
Smeenk, R.J.T.2
Kater, L.3
-
5
-
-
0027994439
-
-
N. Haneji, H. Hamano, K. Yanagi, Y. Hayashi, J. Immunol. 153, 2769 (1994).
-
(1994)
J. Immunol.
, vol.153
, pp. 2769
-
-
Haneji, N.1
Hamano, H.2
Yanagi, K.3
Hayashi, Y.4
-
6
-
-
1842297850
-
-
note
-
The salivary gland homogenates from 3d-Tx NFS/sld mice were prepared in 20 mM tris-HCl buffer (pH 7.2) containing 0.15 M NaCl and proteinase inhibitors (5 mM benzamidine-HCl, 2 mM diisopropyl fluoride, and 2 mM EDTA). The supernatant of the homogenates was fractionated on a Superose 12HR column (Pharmacia) and further purified on a DEAE-Cosmogel column (Nakarai, Japan). SDS-polyacrylamide gel electrophoresis was carried out in 10% gels as described by (28).
-
-
-
-
7
-
-
0025254086
-
-
R. T. Moon and A. P. McMahon, J. Biol. Chem. 265, 4427 (1990); C. S. Birkenmeier et al., Proc. Natl. Acad. Sci. U.S.A. 82, 5671 (1985); M. D. Adams et al., Science 252, 1651 (1991).
-
(1990)
J. Biol. Chem.
, vol.265
, pp. 4427
-
-
Moon, R.T.1
McMahon, A.P.2
-
8
-
-
1842327099
-
-
R. T. Moon and A. P. McMahon, J. Biol. Chem. 265, 4427 (1990); C. S. Birkenmeier et al., Proc. Natl. Acad. Sci. U.S.A. 82, 5671 (1985); M. D. Adams et al., Science 252, 1651 (1991).
-
(1985)
Proc. Natl. Acad. Sci. U.S.A.
, vol.82
, pp. 5671
-
-
Birkenmeier, C.S.1
-
9
-
-
0025879424
-
-
R. T. Moon and A. P. McMahon, J. Biol. Chem. 265, 4427 (1990); C. S. Birkenmeier et al., Proc. Natl. Acad. Sci. U.S.A. 82, 5671 (1985); M. D. Adams et al., Science 252, 1651 (1991).
-
(1991)
Science
, vol.252
, pp. 1651
-
-
Adams, M.D.1
-
10
-
-
1842368201
-
-
note
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Single-letter abbreviations for the amino acid residues are as follows: A, Ala; C, Cys; D, Asp; E, Glu; F, Phe; G, Gly; H, His; I, Ile; K, Lys; L, Leu; M, Met; N, Asn; P, Pro; Q, Gln; R, Arg; S, Ser; T, Thr; V, Val; W, Trp; and Y, Tyr.
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11
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1842405701
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note
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3H]thymidine was added per well, and the incorporated radioactivity was determined.
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12
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1842290073
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note
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The amounts of IFN-γ and IL-2 produced were measured by enzyme-linked immunosorbent assay (ELISA) (29) with IFN-γ - specific monoclonal antibodies (Pharmingen) and mlL-2 ELISA system (Amersham).
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13
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1842333579
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note
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A synthetic peptide of α-fodrin corresponding to the identified 20-amino acid residues [RQKLEDSYRFQFFQRDAEEL) (6)] was produced with an Applied Biosystems model 430A peptide synthesizer. Polyclonal rabbit antibody to the synthetic peptide was developed and affinity purified. Rabbit serum to the synthetic peptide was diluted 1:25 when used for protein immunoblots.
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14
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1842293634
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note
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For immunohistochemistry, formalin-fixed paraffin-embedded salivary gland sections were deparaffinized in xylen and hydrated in ethanol. Sections were stained with polyclonal rabbit antibody to synthetic α-fodrin peptide diluted 1:500 dilution in 10% (v/v) fetal bovine serum, followed by incubation with biotin-labeled goat serum to rabbit as the linking antibody. After the preparations were rinsed, binding was detected with an avidin-biotin-peroxidase complex (Vector Laboratories, Burlingame, CA). Specificity of the staining was confirmed by the observation that incubating polyclonal rabbit antiserum with synthetic peptide completely blocked staining.
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15
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1842294857
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note
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JS-1 cDNA encoding the human nonerythroid α-fodrin cDNA was constructed by inserting cDNA (base pairs 1 through 1784) into the Eco RI site of plasmid pGEX-2T (Pharmacia). GST fusion protein was expressed and purified with a GST gene fusion system (Pharmacia) as described (30). Protein immunoblots were performed and proliferative T cell responses were determined as described in the legend to Fig. 1.
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-
-
-
17
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1842332979
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note
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3H]thymidine was added per well. Incorporation was measured by liquid scintillation counting.
-
-
-
-
19
-
-
0021847682
-
-
D. Perrin and D. Aunis, Nature 315, 589 (1985); D. Perrin, O. K. Langley, D. Aunis, ibid. 326, 498 (1987).
-
(1985)
Nature
, vol.315
, pp. 589
-
-
Perrin, D.1
Aunis, D.2
-
20
-
-
0023135270
-
-
D. Perrin and D. Aunis, Nature 315, 589 (1985); D. Perrin, O. K. Langley, D. Aunis, ibid. 326, 498 (1987).
-
(1987)
Nature
, vol.326
, pp. 498
-
-
Perrin, D.1
Langley, O.K.2
Aunis, D.3
-
21
-
-
0026515691
-
-
D. Perrin, K. Möller, K. Hanke, H.-D. Söling, J. Cell Biol. 116, 127 (1992).
-
(1992)
J. Cell Biol.
, vol.116
, pp. 127
-
-
Perrin, D.1
Möller, K.2
Hanke, K.3
Söling, H.-D.4
-
28
-
-
0022414831
-
-
L. Portmann, N. Hamada, G. Heinrich, L. J. Degroot, J. Clin. Endocrinol. Metab. 6, 1001 (1985).
-
(1985)
J. Clin. Endocrinol. Metab.
, vol.6
, pp. 1001
-
-
Portmann, L.1
Hamada, N.2
Heinrich, G.3
Degroot, L.J.4
-
29
-
-
0025039679
-
-
S. Backkeskov et al., Nature 347, 151 (1990); R. Tisch et al., ibid. 366, 72 (1993).
-
(1990)
Nature
, vol.347
, pp. 151
-
-
Backkeskov, S.1
-
30
-
-
0027908753
-
-
S. Backkeskov et al., Nature 347, 151 (1990); R. Tisch et al., ibid. 366, 72 (1993).
-
(1993)
Nature
, vol.366
, pp. 72
-
-
Tisch, R.1
-
31
-
-
0023867650
-
-
F. A. Karlsson, P. Burman, L. Loot, S. Mardh, J. Clin. Invest. 81, 475 (1988).
-
(1988)
J. Clin. Invest.
, vol.81
, pp. 475
-
-
Karlsson, F.A.1
Burman, P.2
Loot, L.3
Mardh, S.4
-
37
-
-
1842331807
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note
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We thank S. Sone and K. Hoil for human sera and R. T. Moon for cDNA. Supported by grants-in-aid for scientic research from the Ministry of Education, Science, and Culture of Japan.
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