PCR ribotyping and arbitrarily primed PCR for the comparison of enterotoxigenic Bacteroides fragilis strains from two Polish university hospitals

Clinical Microbiology and Infection

Volumn 3, Issue 1, 1997, Pages 102-108

  Martirosian, Gayane ^a   Van Belkum, Alex ^b   Van Leeuwen, Willem ^b   Meisel Mikolajczyk, Felicja ^a   Verbrugh, Henri ^b  

^a MEDICAL UNIVERSITY OF LUBLIN   (Poland)

^b ERASMUS MEDICAL CENTER   (Netherlands)

Author keywords

Arbitrarily primed PCR; Bacteroides fragilis; Enterotoxin; Molecular typing; PCR ribotyping

Indexed keywords

BACTERIAL DNA; ENTEROTOXIN;

ARTICLE; BACTERIAL INFECTION; BACTERIUM CULTURE; BACTERIUM IDENTIFICATION; BACTERIUM ISOLATION; BACTEROIDES FRAGILIS; COMPARATIVE STUDY; DIARRHEA; FECES ANALYSIS; FEMALE; HOSPITAL INFECTION; HUMAN; INFECTION RATE; MAJOR CLINICAL STUDY; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; RIBOTYPING; VAGINA;

BACTERIA (MICROORGANISMS); BACTEROIDES FRAGILIS;

EID: 0030998576     PISSN: 1198743X     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1469-0691.1997.tb00258.x     Document Type: Article

References (30)

1. Holdeman LV, Good IJ, Moore WEC. Human faecal flora: variation in bacterial composition within individuals and a possible effect of emotional stress. Appl Environ Microbiol 1976; 31: 356-75.
2. Drasar BS, Hill MJ. (eds). Human intestinal flora. London: Academic Press, 1974.
3. Simon GL, Gorbach SL. Intestinal health and disease. Gastroenterology 1984; 86: 174-93.
4. Willis AT. Abdominal sepsis. In Duerden BJ, Drasar BS eds. Anaerobes in human disease. London: Edward Arnold, 1991: 197-223.
5. Kato N, Karuniawati A, Jotwani R, Kato H, Waranabe K, Ueno K. Isolation of enterotoxigenic Bacteroides fragilis strains from extraintestinal sites by cell culture assay. Clin Infect Dis 1995; 20 (Suppl 2): S141.
6. Myers LL, Firehammer BD, Shoop DS, Border MM. Baaeroides fragilis: a possible cause of acute diarrhocal disease in newborn lambs. Infect Immun 1984; 44: 241-4.
7. Myers LL, Shoop DS, Firehammer BD, Border MM. Association of enterotoxigenic Baaeroides fragilis with diarrhoeal disease in calves. Am J Vet Res 1987; 48: 1344-7.
8. Myers LL, Shoop DS. Association of enterotoxigenic Bacieroides fragilis with diarrhoeal disease in young pigs. Am J Vet Res 1987; 48: 774-6.
9. Myers LL, Shoop DS, Byars TD. Diarrhoea, associated with enterotoxigenic Bacteroides fragilis in foals. Am J Vet Res 1987, 48: 1565-7.
10. Joaquin VH, Griffes JG, Lee C, Sears CL. Association of Baaeroides fragilis with childhood diarrhoea. Scand J Infect Dis 1995; 27: 211-15.
11. Sears CL, Myers LL, Lazebny A, Van Tassel RL. Enterotoxigenic Bacteroides fragilis. Clin Infect Dis 1995; 20: 142-8.
12. Pantosti A, Cerquetti M, Colangeli R, Ambrosio F. Detection of intestinal and extra-intestinal strains of enterotoxigenic Bacteroides fragilis by the HT-29 cytotoxicity assay. J Med Microbiol 1994; 41 191-6.
13. Meisel-Mikolajczyk F, Sebald M, Torbicka E, Rafalowska K, Zielinska U. Isolation of enterotoxigenic Baaeroides fragilis strains in Poland. Acta Microbiol Polon 1994; 43: 389-92.
14. Maslow JN, Mulligan ME, Arbeit RD. Molecular epidemiology, application of contemporary techniques to the typing of microorganisms. Clin Infect Dis 1993; 17: 1553-64.
15. Van Belkum A. DNA fingerprinting of medically important microorganisms by use of PCR. Clin Microbiol Rev 1994; 7: 174-184
16. Van Belkum A, Homan W, Limper L, Quint WGV Genotyping isolates and clones of Giardia duodenalis by polymerase chain reaction: implications for the detection of genetic variation among protozoan parasite species. Mol Biochem Parasitol 1993; 61: 6978-85.
17. Martirosian G, Kuipers S, Verbrugh H, van Belkum A, Meisel-Mikolajczyk F. PCR ribotyping and arbitrarily primed PCR for typing strains of Clostridium difficile from a Polish Maternity Hospital. J Clin Microbiol 1995; 33: 2016-21.
18. Kristjansson KM, Samore MH, Gerding DN, et al. Comparison of restriction endonuclease analyses, ribotyping and pulsed field gel electrophoresis for molecular differentiation of Clostridium difficile strains. J Clin Microbiol 1994; 32: 1963-9.
19. Gueler V. Typing of Clostridium difficile strains by PCR amplification of variable length 16S-23S rDNA regions. J Gen Microbiol 1993; 139: 3089-97.
20. Myers LL, Shoop BS. Antigenic characteristics of enterotoxigenic and nonenterotoxigenic isolates of Bacteroides fragilis. Am J Vet Res 1987; 48: 643-5.
21. Van Tassel RL, Lyerly DM, Wilkins TD. Purification and characterization of an enterotoxin from Bacteroides fragilis. Infect Immun 1991: 80: 1343-50.
22. Weikel CS, Grieco FD, Reuben J, Myers LL, Sack RB. Human colonic epithelial cells, HT-29/C1, treated with crude Bacteroides fragilis enterotoxin dramatically alter their morphology. Infect Immun 1992; 60: 321-7.
23. Boom B, Sol CJA, Salimans MMM, Jansen CL, Wertheim PME, Van der Noordaa J. Rapid and simple method for purification of nucleic acids. J Clin Microbiol 1990; 28: 495-503.
24. Van Belkum A, Ouint WG, Pauw BE. Melchers WJG, Meis JF. Typing of Aspergillus species and Aspergillus fumigatus isolated by interrepeat polymerase chain reaction. J Clin Microbiol 1993; 11: 2502-5.
25. Versalovic J, Koeuth T, Lupski JR. Distribution of repetitive DNA sequences in eubacteria and application to finger-printing of bacterial genomes. Nucleic Acids Res 1991; 19: 6823-31.
26. Moncrief JS, Obiso R, Barroso LA, et al. The enterotoxin of Bacteroides fragilis is a metalloprotease. Infect Immun 1995; 63: 175-81.
27. Ingham HR, Baton S, Venables CW, Adams PC. Bacteroides fragilis resistant to metronidazole after long term therapy. Lancet 1988; 1: 214.
28. Meisel-Mikolajczyk F, Rouyan GHS, Henry C, Sebald M. Enterotoxigenic B. fragilis from France and Poland [Abstract 62]. In: World Congress of Anaerobic Bacteria and Infections, San Juan, Puerto RJCO, 1995.
29. Marianowski L, Leszczynski P, Dworczynska M, Cwyl-Zembrzuska L, Meisel-Mikolajczk F. Colonisation of the female genital tract of pregnancy pathology patients with Bacteroides fragilis strains. In: Program and Abstracts of the First International Meeting of Practical Obstetrics, Paris, France. 1993.
30. Podglajen I, Breuil J, Casin I, Collatz E. Genotypic identification of two groups within the species Bacteroides fragilis by ribotyping and by analysis of PCR-generated fragment patterns and insertion sequence content. J Bacteriol 1995: 177. 5270-5.