Genetic approach to regulated exocytosis using functional complementation in Paramecium: Identification of the ND7 gene required for membrane fusion

Molecular Biology of the Cell

Volumn 8, Issue 6, 1997, Pages 1063-1071

  Skouri, Fériel ^a   Cohen, Jean ^a  

^a CNRS   (France)

Author keywords

[No Author keywords available]

Indexed keywords

ANIMAL CELL; ARTICLE; CONTROLLED STUDY; EXOCYTOSIS; GENE ISOLATION; GENETIC COMPLEMENTATION; HUMAN; MEMBRANE FUSION; PARAMECIUM; PRIORITY JOURNAL; SECRETORY GRANULE;

ANIMALIA; PARAMECIUM;

EID: 0030997642     PISSN: 10591524     EISSN: None     Source Type: Journal    
DOI: 10.1091/mbc.8.6.1063     Document Type: Article

References (47)

1. Altschul, S.F., Gish, W., Miller, W., Myers, E.W., and Lipman, D.J. (1990). Basic local alignment search tool. J. Mol. Biol. 215, 403-410.
2. Aufderheide, K.J. (1978). The effective site of some mutations affecting exocytosis in Paramecium tetraurelia. Mol. Gen. Genet. 165, 199-205.
3. Aunis, D.J., Hesketh, R., and Devilliers, G. (1979). Freeze-fracture study of chromaffin cell during exocytosis: evidence for connections between the plasma membrane and secretory vesicles and for movements of plasma membrane associated particles. Cell Tissue Res. 197, 433-441.
4. Beisson, J., Cohen, J., Lefort-Tran, M., Pouphile, M., and Rossignol, M. (1980). Control of membrane fusion in exocytosis. Physiological studies on a Paramecium mutant blocked in the final step of the trichocyst extrusion process. J. Cell Biol. 85, 213-227.
5. Beisson, J., Lefort-Tran, M., Pouphile, M., Rossignol, M., and Satir, B. (1976). Genetic analysis of membrane differeniation in Paramecium. Freeze-fracture study of the trichocyst cycle in wild type and mutant strains. J. Cell Biol. 69, 126-143.
6. Bennett, M.K., and Scheller, R.H. (1993). The molecular machinery for secretion is conserved from yeast to neurons. Proc. Natl. Acad. Sci. USA 90, 2559-2563.
7. Bonnemain, H., Gulik-Krzywicki, T., Grandchamp, C., and Cohen, J. (1992). Interactions between genes involved in exocytotic membrane fusion in Paramecium. Genetics 130, 461-470.
8. Bourgain, F., and Katinka, M. (1991). Telomeres inhibit end to end fusion and enhance maintenance of linear DNA molecules injected into the Paramecium primaurelia macronucleus. Nucleic Acids Res. 19, 1541-1547.
9. Burgoyne, R.D., and Morgan, A. (1993). Regulated exocytosis. Biochem. J. 293, 305-316.
10. Caron, F., and Meyer, E. (1985). Does Paramecium primaurelia use a different genetic code in its macronucleus? Nature 314, 185-188.
11. Chandler, D.E., and Heuser, J. (1980). Arrest of membrane fusion events in mast cells by quick freezing. J. Cell Biol. 86, 666-674.
12. Cohen, J., and Beisson, J. (1980). Genetic analysis of the relationships between the cell surface and the nuclei in Paramecium tetraurelia. Genetics 95, 797-818.
13. Devereux, J., Haeberli, P., and Smithies, O. (1984). A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res. 12, 387-395.
14. Dupuis, P. (1992). The beta-tubulin genes of paramecium are interrupted by two 27 bp introns. EMBO J. 22, 3713-3719.
15. Geourjon, C., and Deleage, G. (1993). Interactive and graphic coupling between multiple alignments, secondary structure predictions and motif/pattern scanning into proteins. Comput. Appl. Biosci. 9, 87-91.
16. Godiska, R., Aufderheide, K., Gilley, D., Hendrie, P., Fitzwater, T., Preer, L., Poliski, B., and Preer, J. (1987). Transformation of Paramecium by microinjection of a cloned serotype. Proc. Natl. Acad. Sci. USA 84, 7590-7594.
17. Görtz, H.-D. (1988). Paramecium. Berlin: Springer-Verlag.
18. Gulyas, B.J. (1980). Cortical granules of mammalian eggs. Int. Rev. Cytol. 63, 357-392.
19. Haynes, W.J., Ling, K.-Y., Saimi, Y., and Kung, C. (1996). Toward cloning genes by complementation in Paramecium. Neurogenetics (in press).
20. Heuser, J.E., Reese, T.S., Dennis, M.J., Jan, Y., Jan, L., and Evans, L. (1979). Synaptic vesicle exocytosis captured by quick freezing and correlated with quantal transmitter release. J. Cell Biol. 81, 275-300.
21. 2+ influx associated with exocytosis is specifically abolished in a Paramecium exocytotic mutant. J. Cell Biol. 111, 2527-2535.
22. Kerbœuf, D., Leberre, A., Dedieu, J.C., and Cohen, J. (1993). Calmodulin is essential for assembling links necessary for exocytotic membrane fusion in paramecium. EMBO J. 12, 3385-3390.
23. Kink, J.A., Maley, M.E., Preston, R.R., Ling, K.-Y., Wallen-Friedman, M.A., Saimi, Y., and Kung, C. (1990). Mutations in Paramecium calmodulin indicate functional differences between the C-terminal and N-terminal lobes in vivo. Cell 62, 165-174.
24. Lefort-Tran, M., Aufderheide, K., Pouphile, M., Rossignol, M., and Beisson, J. (1981). Control of exocytotic processes: cytological and physiological studies of trichocyst mutants in Paramecium tetraurelia. J. Cell Biol. 88, 301-311.
25. Lupas, A., van Dyke, M., and Stock, J. (1991). Predicting coiled coils from protein sequences. Science 252, 1162-1164.
26. Marck, C. (1988). 'DNA strider': a 'C' program for the fast analysis of DNA and protein sequence on the Apple Macintosh family of computers. Nucleic Acids Res. 16, 1829-1836.
27. McDevitt, M., Hart, R., Wong, W., and Nevins, J. (1986). Sequences capable of restoring poly(A) site function define two distinct downstream elements. EMBO J. 5, 2907-2013.
28. Nonet, M.L., Grundahl, K., Meyer, B.J., and Rand, J.B. (1993). Synaptic function is impaired but not eliminated in C. elegans mutants lacking synaptotagmin. Cell 73, 1291-1305.
29. O'Neil, K.T., and DeGrado, W.F. (1990). How calmodulin binds its targets: sequence independent recognition of amphiphilic alpha-helices. Trends Biochem. Sci. 15, 59-64.
30. Pape, R., and Plattner, H. (1985). Synchronous exocytosis in Paramecium cells. V. Ultrastructural adaptation phenomena during reinsertion of secretory organelles. Eur. J. Cell Biol. 36, 38-47.
31. Persson, B., and Argos, P. (1994). Prediction of transmembrane segments in proteins utilising multiple sequence alignments. J. Mol. Biol. 237, 182-192.
32. Pieribone, V.A., Shupliakov, O., Brodin, L., Hilfiker-Rothenfluth, S., Czernik, A.J., and Greengard, P. (1995). Distinct pools of synaptic vesicles in neurotransmitter release. Nature 375, 493-497.
33. Plattner, H., Miller, F., and Bachman, L. (1973). Membrane specializations in the form of regular membrane to membrane attachment sites in Paramecium. A correlated freeze-etching and ultrathin-sectioning analysis. J. Cell Sci. 13, 687-719.
34. Pouphile, M., Lefort-Tran, M., Plattner, H., Rossignol, M., and Beisson, J. (1986). Genetic dissection of the morphogenesis of exocytosis sites in Paramecium. Biol. Cell 56, 151-162.
35. Preer, L., Hamilton, G., and Preer, J. (1992). Micronuclear DNA from Paramecium tetraurelia: serotype 51A gene has internally eliminated sequences. J. Protozool. 39, 678-682.
36. Preer, J.R., Jr., Preer, L.B., Rudman, B.M., and Barnett, A.J. (1985). Deviation from the universal code shown by the gene for surface protein 51A in Paramecium. Nature 314, 188-190.
37. Rost, B., and Sander, C. (1993). Prediction of protein secondary structure at better than 70% accuracy. J. Mol. Biol. 232, 584-599.
38. Rothman, J. (1994). Mechanisms of intracellular protein transport. Nature 372, 55-63.
39. Russel, C., Fraga, D., and Hinrichsen, R. (1994). Extremely short 20-33 nucleotide introns are the standard length in Paramecium tetraurelia. Nucleic Acids Res. 22, 1221-1225.
40. Sainsard, A., Claisse, M., and Balméfrézol, M. (1974). A nuclear mutation affecting structure and function of mitochondria in Paramecium. Mol. Gen. Genet 130, 113-125.
41. Sakaguchi, M., Mihara, K., and Sato, R. (1987). A short amino terminal segment of microsomal cytochrome P-450 functions both as an insertion signal and as a stop-transfer sequence. EMBO J. 6, 2425-2431.
42. Sonneborn, T.M. (1970). Methods in Paramecium research. Methods Cell Physiol. 4, 241-339.
43. Sonneborn, T.M. (1974). Paramecium aurelia. Handbook of Genetics, 2nd ed., New York: Plenum Press, 469-594.
44. Sonneborn, T.M. (1975). The Paramecium aurelia complex of 14 sibling species. Trans. Am. Microsc. Soc. 94, 155-178.
45. Südhof, T. (1995). The synaptic vesicle cycle: a cascade of protein-protein interactions. Nature 375, 645-653.
46. von Heijne, G. (1986). A new method for predicting signal sequence cleavage sites. Nuclei Acids Res. 14, 4683-4690.
47. White, J.V., Stultz, C.M., and Smith, T.F. (1994). Protein classification by stochastic modeling and optimal filtering of amino-acid sequences. Math. Biosci. 119, 35-75.