Cloning, expression, purification and characterization of triosephosphate isomerase from Trypanosoma cruzi

European Journal of Biochemistry

Volumn 244, Issue 3, 1997, Pages 700-705

  Ostoa Saloma, Pedro ^a   Garza Ramos, Georgina ^a   Ramírez, Jorge ^a   Becker, Ingeborg ^b   Berzunza, Myriam ^b   Landa, Abraham ^b   Gomez Puyou, Armando ^a   Tuena De Gómez Puyou, Marietta ^a   Pérez Montfort, Ruy ^a  

^a INSTITUTO DE FISIOLOGÍA CELULAR   (Mexico)

^b UNIVERSIDAD NACIONAL AUTÓNOMA DE MÉXICO   (Mexico)

Author keywords

Characterization; Purification; Sequence; Triosephosphate isomerase; Trypanosoma cruzi

Indexed keywords

MESYLIC ACID METHYL ESTER; TRIOSEPHOSPHATE ISOMERASE;

AMINO ACID SEQUENCE; ARTICLE; CIRCULAR DICHROISM; ENZYME PURIFICATION; ENZYME STRUCTURE; GENE SEQUENCE; MOLECULAR CLONING; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; SEQUENCE ANALYSIS; TRYPANOSOMA CRUZI;

ESCHERICHIA COLI; LEISHMANIA MEXICANA; TRYPANOSOMA; TRYPANOSOMA BRUCEI; TRYPANOSOMA CRUZI; TRYPANOSOMATIDAE;

EID: 0030997077     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1432-1033.1997.00700.x     Document Type: Article

References (27)

1. Wang, C. C. (1995) Molecular mechanisms and therapeutic approaches to the treatment of african trypanosomiasis, Annu. Rev. Pharmacol. Toxicol. 35, 93-127.
2. Fairlamb, A. H. & Cerami, A. (1992) Metabolism and function of trypanothione in the kinetoplastid, Annu. Rev. Microbiol. 46, 695-729.
3. Michels, P. A. M. & Hannaert, V. (1994) The evolution of kinetoplastid glycosomes. J. Bioenerg. Biomembr. 26, 213-219.
4. Verlinde, C. L. J., Merrit, E. A., van der Akker, F., Kim, H., Feil, I., Delboni, L. F., Mande, S. C., Sarfaty, S., Petra, P. H. & Hol, W. G. J. (1994) Protein crystallography and infectious diseases, Protein Sci. 3, 1670-1686.
5. Visser, N. & Opperdoes, F. R. (1980) Glycolysis in Trypanosoma brucei, Eur. J. Biochem. 103, 623-632.
6. Wierenga, R. K., Noble, M. E. M. & Davenport, R. C. (1992) Comparison of the refined crystal structures of liganded and unliganded chicken, yeast, and trypanosoma TPI, J. Mol. Biol. 224, 1115-1126.
7. Misset, O., Van Beeumen, J., Lambeir, A. M., Van Der Meer, R. & Opperdoes, F. R. (1987) Glyceraldehyde-phosphate dehydrogenase from Trypanosoma brucei. Comparison of the glycosomal and cytosolic enzymes, Eur. J. Biochem. 162, 501-507.
8. Misset, O. & Opperdoes, F. R. (1987) The phosphoglycerate kinases from Trypanospma brucei. A comparison of the glycosomal and the cytosolic isoenzymes and their sensitivity towards suramin, Eur. J. Biochem. 162, 493-500.
9. Noble, M. E. M., Wierenga, R. W., Lambeir, A. M., Opperdoes, F. R., Thunnisen, A. M. W. H., Kalk, K. H., Groendijk, M. & Hol, W. G. J. (1991) The adaptability of the active site of trypanosomal triosephosphate isomerase as observed in the crystal structures of three different complexes, Proteins Struct. Funct. Genet. 10, 50-69.
10. Cazzulo, J. J. (1994) Intermediate metabolism in Trypanosoma cruzi, J. Bioenerg. Biomembr. 26, 157-165.
11. Gómez-Puyou, A., Saavedra-Lira, E., Becker, I., Zubillaga, R. A., Rojo-Domínguez, A. & Perez-Montfort, R. (1995) Using evolutionary changes to achieve species specific inhibition of enzyme action-studies with triose phosphate isomerase, Chemistry & Biology 2, 847-855.
12. Kohl, L., Callens, M., Wierenga, R. K., Opperdoes, F. R. & Michels, P. A. M. (1994) Triosephosphate isomerase of Leishmania mexicana. Cloning and characterization of the gene, overexpression in Escherichia coli and analysis of the protein, Eur. J. Biochem. 220, 331-338.
13. Alonso, G., Guevara, P. & Ramirez, J. L. (1992) Trypanosomatidae codon usage and GC distribution, Mem. Inst. Oswaldo Cruz 87, 517-523.
14. McCarthy-Burke, C., Taylor, Z. A. & Buck, G. A. (1989) Characterization of the spliced leader genes and transcripts in Trypanosoma cruzi, Gene (Amst.) 82, 177-189.
15. Sambrook, J., Fritsch, E. F. & Maniatis, T. (1989) Molecular cloning: a laboratory manual, 2nd edn. Cold Spring Harbor Laboratory, Cold Spring Harbor NY.
16. Borchert, T. V., Prett, K., Zeelen, J. P., Callens, M., Noble, M. E. M., Opperdoes, F. R., Michels, P. A. M. & Wierenga, R. (1993) Overexpression of trypanosomal triosephoshate isomerase in Escherichia coli, and characterization of a dimer-interface mutant, Eur J. Biochem. 211, 703-710.
17. Laemmli, U. K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4, Nature 227, 680-685.
18. Bradford, M. M. (1976) A rapid and sensitive method for the quantitation of protein-dye binding, Anal. Biochem. 72, 248-254.
19. Pace, N. C., Vajdos, F., Fee, L., Grimsley, G. & Gray, V. (1995) How to measure and predict the molar absorption coefficient of a protein, Protein Sci. 4, 2411-2423.
20. Swinkels, B. W., Gibson, W. C., Osinya, K. A., Kramer, R., Veeneman, G. H., Van Boom, J. H. & Borst, P. (1986) Characterization of the gene for the microbody (glycosomal) triosephosphate isomerase of Trypanosoma brucei, EMBO J. 5, 1291-1298.
21. Wierenga, R. K., Noble, M. E. M., Vriend, G., Nauche, S. & Hol, W. G. J. (1991) Refined 1.83 Å structure of trypanosomal triosephosphate isomerase crystallized in the presence of 2.4 M ammonium sulphate. A comparison with the structure of trypanosomal triosephosphate isomerase-glycerol-3-phosphate complex, J. Mol. Biol. 220, 995-1015.
22. Garza-Ramos, G., Pérez-Montfort, R., Rojo-Domínguez, A., Tuena de Gómez-Puyou, M. & Gómez-Puyou, A. (1996) Species-specific inhibition of homologous enzymes by modification of non-conserved amino acids. The Cys residues of triosephosphate isomerase, Eur. J. Biochem. 241, 114-120.
23. Kohlhoff, M., Dahm, A. & Hensel, R. (1996) Tetrameric triosephosphate isomerase from hyperthermophilic archaea, FEBS Lett. 383, 245-250.
24. Waley, S. G. (1973) Refolding of triosephosphate isomerase, Biochem. J. 135, 165-172.
25. Zabori, S., Rudolph, A. & Jaenicke, R. (1980) Folding and association of triosephosphate isomerase from rabbit muscle, Z. Naturforsch. 35, 999-1004.
26. Garza-Ramos, G., Tuena de Gómez-Puyou, M., Gómez-Puyou, A. & Gracy, R. W. (1992) Dimerization and reactivation of triosephosphate isomerase in reverse micelles, Eur. J. Biochem. 208, 389-395.
27. Mainfroid, V., Terpstra, P., Beauregard, M., Frere, J. M., Mande, S. C., Hol, W. G. J., Martial, J. A. & Goraj, K. (1996) Three hTIM mutants that provide new insights on why TIM is a dimer, J. Mol. Biol. 257, 441-456.