Discrimination of late apoptotic/necrotic cells (Type III) by flow cytometry in solid tumors

Cytometry

Volumn 28, Issue 1, 1997, Pages 81-89

  O'Brien, Melissa C ^a,f   Healy Jr , Stephen F ^b   Raney, Shula R ^b   Hurst, Josephine M ^b   Avner, Barry ^c   Hanly, Andrew ^d   Mies, C ^d   Freeman, James W ^e   Snow, Christopher ^a   Koester, Steven K ^a   Bolton, Wade E ^a  

^a Department of Scientific Research   (United States)

^b Goodwin Institute   (United States)

^c Baptist Med Ctr Dept of Oncology   (United States)

^d JACKSON MEMORIAL HOSPITAL   (United States)

^e UNIVERSITY OF KENTUCKY   (United States)

^f Shell Services Company   (United States)

Author keywords

Apoptosis; Flow cytometry; Necrosis; Tubulin; Tumors

Indexed keywords

PROPIDIUM IODIDE; TUBULIN; TUBULIN ANTIBODY; UNCLASSIFIED DRUG;

ANIMAL EXPERIMENT; ANIMAL MODEL; APOPTOSIS; ARTICLE; BREAST CARCINOMA; CONTROLLED STUDY; HUMAN; HUMAN CELL; MOUSE; NECROSIS; NONHUMAN; PRIORITY JOURNAL; SOLID TUMOR;

ANIMALS; APOPTOSIS; BREAST NEOPLASMS; CELL HYPOXIA; CELL SEPARATION; CELL SURVIVAL; DNA, NEOPLASM; ELECTROPHORESIS, AGAR GEL; FLOW CYTOMETRY; FLUORESCENT ANTIBODY TECHNIQUE; HUMANS; MAMMARY NEOPLASMS, EXPERIMENTAL; MICE; NECROSIS; PLOIDIES; PROPIDIUM; TUBULIN; TUMOR CELLS, CULTURED;

ANIMALIA;

EID: 0030996827     PISSN: 01964763     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0320(19970501)28:1<81::AID-CYTO10>3.0.CO;2-N     Document Type: Article

References (39)

1. Afanasyev VN, Korol BA, Matylevich NP, Pechatnikov VA, Umansky SR: The use of flow cytometry for the investigation of cell death. Cytometry 14:603-609, 1993.
2. Bauer TW, Tubbs, RR, Edinger MG, Suit PF, Gephardt GN, Levin HS: A prospective comparison of DNA quantitation by image and flow cytometry. Am J Clin Pathol 93:322-326, 1990.
3. Belloc F, Dumain P, Boisseau MR, Jalloustre C, Reiffers J, Bernard P, Lacombe F: A flow cytometric method using Hoechst 33342 and propidium iodide for simultaneous cell cycle analysis and apoptosis determination in unfixed cells. Cytometry 17:59-65, 1994.
4. Boltz RC, Fischer PA, Wicker LS, Peterson LB: Single UV excitation of Hoechst 33342 and ethidium bromide for simultaneous cell cycle analysis and viability determinations on in vitro cultures of murine B lymphocytes. Cytometry 15:28-34, 1994.
5. Clevenger CV, Shankey TV: Cytochemistry II: Immunofluorescence measurement of intracellular antigens. In: Clinical Flow Cytometry: Principles and Application, Bauer KD, Duque RE, Shankey TV (eds). Williams & Wilkins, Baltimore, 1993, pp 157-175.
6. Cohen JJ: Apoptosis. Immunol Today 14:126-130, 1993.
7. Columbano A: Cell death: Current difficulties in discriminating apoptosis from necrosis in the context of pathological processes in vivo. J Cellular Biochem 58:181-190, 1995.
8. Darzynkiewicz Z, Bruno S, Del Bino G, Gorczyca W, Hotz MA, Lassota P, Traganos F: Features of apoptotic cells measured by flow cytometry. Cytometry 13:795-808, 1992.
9. Dive C, Gregory CD, Phipps DJ, Evans DL, Milner AE, Wyllie AH: Analysis and discrimination of necrosis and apoptosis (programmed cell death) by multiparameter flow cytometry. Biochim Biophys Acta 1133:275-285, 1992.
10. Dolzhanskiy A, Basch RS: Flow cytometric determination of apoptosis in heterogeneous cell populations. J Immunol Methods 180:131-140, 1995.
11. Elstein KH, Zucker RM: Comparison of cellular and nuclear flow cytometric techniques for discriminating apoptotic subpopulations. Exp Cell Res 211:322-331, 1994.
12. Endresen PC, Prytz PS, Aarbakke J: A new flow cytometric method for discrimination of apoptotic cells and detection of their cell cycle specificity through staining of F-actin and DNA. Cytometry 20:162-171, 1995.
13. Gold R, Schmied M, Rothe G, Zischler H, Breitschopf H, Wekerle H, Lassmann H: Detection of DNA fragmentation in apoptosis: Application of in situ nick translation to cell culture systems and tissue sections. J Histochem Cytochem 41:1023-1030, 1993.
14. Gorczyca W, Tuziak T, Kram A, Melamed MR, Darzynkiewicz Z: Detection of apoptosis-associated DNA strand breaks in fine-needle aspiration biopsies by in situ end labeling of fragmented DNA. Cytometry 15:169-175, 1994.
15. Gorczyca W, Gong J, Darzynkiewicz Z: Detection of DNA strand breaks in individual apoptotic cells by the in situ terminal deoxynucleotidyl transferase and nick translation assays. Cancer Res 53:1945-1951, 1993.
16. Hedley DW, Clark GM, Corneliss CJ, Killander D, Kute T, Merkel D: Consensus review of the clinical utility of DMA cytometry in carcinoma of the breast. Cytometry 14:482-485, 1993.
17. Kubbies M: Flow cytometric DNA-histogram analysis: Non-stoichiometric fluorochrome binding and pseudo-aneuploidy. J Pathol 167:413-419, 1992.
18. Lockshin RA, Zakeri ZF: Physiology and protein synthesis in programmed cell death: Early synthesis and DNA degradation. Ann NY Acad Sci 663:234-249, 1992.
19. Lyons AB, Samuel K, Sanderson A, Maddy AH: Simultaneous analysis of immunophenotype and apoptosis of murine thymocytes by single laser flow cytometry. Cytometry 13:809-821, 1992.
20. Meyn RE, Stephens LC, Ang KK, Hunter NR, Brock WA, Milas L, Peter LJ: Heterogeneity in the development of apoptosis in irradiated murine tumours of different histologies. Int J Radiat Biol 64:583-591, 1993.
21. O'Brien MC, Bolton WE: Comparison of cell viability probes compatible with fixation and permeabilization for combined surface and intracellular staining in flow cytometry. Cytometry 19:243-255, 1995.
22. O'Brien MC, Gupta RK, Lee SY, Bolton WE: Use of a multiparametric panel to target subpopulations in a heterogeneous solid tumor model for improved analytical accuracy. Cytometry 21:76-83, 1995.
23. Ojeda F, Guarda MI, Maldonado C, Folch H: A flow-cytometric method to study DNA fragmentation in lymphocytes. J Immunol Methods 152:171-176, 1992.
24. Ormerod MG, Sun XM, Snowden RT, Davies R, Fearnhead H, Cohen GM: Increased membrane permeability of apoptotic thymocytes: A flow cytometric study. Cytometry 14:595-602, 1993.
25. Riedy MC, Muirhead KA, Jensen CP, Stewart CC: Use of a photolabeling technique to identify nonviable cells in fixed homologous or heterologous populations. Cytometry 12:133-139, 1991.
26. Schmid I, Krall WJ, Uittenbogaart CH, Braun J, Giorgi JV: Dead cell discrimination with 7-amino-actinomycin D in combination with dual color immunofluorescence in single laser flow cytometry. Cytometry 13:204-208, 1992,
27. Schmid I, Uittenbogaart CH, Giorgi JV: Sensitive method for measuring apoptosis and cell surface phenotype in human thymocytes by flow cytometry. Cytometry 15:12-20, 1994.
28. Shankey TV, Rabinovitch PS, Bagwell B, Bauer KD, Duque RE, Hedley DW, Mayall BH, Wheeless L: Guidelines for implementation of DNA cytometry. Cytometry 14:472-477, 1993.
29. Steck KD, McDonnell TJ, El-Naggar AK: Flow cytometric analysis of apoptosis and BCL-2 in human solid neoplasms. Cytometry 20:154-161, 1995.
30. Telford WG, King LE, Fraker PJ: Comparative evaluation of several DNA binding dyes in the detection of apoptosis-associated chromatin degradation by flow cytometry. Cytometry 13:137-143, 1992.
31. Terstappen LWMM, Shah VO, Conrad MP, Recktenwald D, Loken MR: Discriminating between damaged and intact cells in fixed flow cytometric samples. Cytometry 9:477-484, 1988.
32. Tounekti O, Belehradek, Jr. J, Mir LM: Relationships between DNA fragmentation, chromatin condensation, and changes in flow cytometry profiles detected during apoptosis. Exp Cell Res 217:506-516, 1995.
33. Van de Loosdrecht AA, Ossenkoppele GJ, Beelen RHJ, Broekhoven MG, Dräger AM, Langenhuijsen MM: Apoptosis in tumor necrosis factor-alpha-dependent, monocyte-mediated leukemic cell death: A functional, morphologic, and flow cytometric analysis. Exp Hematol 21:1628-39, 1993.
34. Vaux, D: Toward an understanding of the molecular mechanisms of physiological cell death. Proc Natl Acad Sci 90:786-789, 1993.
35. Visscher DW, Zarbo RJ, Jacobsen G, Kambouris A, Talpos G, Sakr W, Crissman JD: Multiparametric deoxyribonucleic acid and cell cycle analysis of breast carcinomas by flow cytometry: Clinicopathologic correlations. Lab Invest 62:370-378, 1990.
36. Wing MG, Montgomery AMP, Songsivilai S, Watson JV: An improved method for the detection of cell surface antigens in samples of low viability using flow cytometry. J Immunol Methods 126:21-27, 1990.
37. Wyllie AH: Apoptosis (the 1992 Frank Rose Memorial Lecture). Br J Cancer 67:205-208, 1993.
38. Zamai L, Bareggi R, Santavenerre E, Vitale M: Subtraction of autofluorescent dead cells from the lymphocyte flow cytometric binding assay. Cytometry 14:951-954, 1993.
39. Zhang L, Wang C, Radvanyi LG, Miller RG: Early detection of apoptosis in defined lymphocyte populations in vivo. J Immunol Methods 181:17-27, 1995.