Isolation, characterization and comparison of a novel crustacean toxin with a mammalian toxin from the venom of the scorpion Centruroides noxius Hoffmann

Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology

Volumn 116, Issue 3, 1997, Pages 315-322

  García, Consuelo ^a   Becerril, Baltazar ^a   Selisko, Barbara ^a   Delepierre, Muriel ^b   Possani, Lourival D ^a  

^a INSTITUTO DE BIOTECNOLOGÍA   (Mexico)

^b INSTITUT PASTEUR   (France)

Author keywords

amino acid sequence; Centruroides noxius; crustacean specific toxin; scorpion toxins; sodium channel; species specificity; three dimensional modelling

Indexed keywords

TOXIN;

ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; BRAIN; CONTROLLED STUDY; DOSE RESPONSE; DRUG ANALYSIS; DRUG ISOLATION; DRUG TOXICITY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; NEUROTOXICITY; NONHUMAN; PRIORITY JOURNAL; RAT; SCORPION;

EID: 0030994091     PISSN: 03050491     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0305-0491(96)00246-5     Document Type: Article

References (55)

1. Catterall, W. Neurotoxins that act on voltage-sensitive sodium channels in excitable membranes. Annu. Rev. Pharmacol. Toxicol. 20:15-43;1980.
2. Rochat, H.; Bernard, P.; Couraud, F. Scorpion toxins: chemistry and mode of action. In Ceccarelli, B.; Clementi, F. (Eds). Advances in Cytopharmacology, Vol. 3B. New York: Raven Press; 1979:325-334.
3. Lazdunski, M.; Frelin, C.; Barhanin, J.; Lombet, A.; Meiri, H.; Pauron, D.; Romey, G.; Schmid, A.; Schweitz, H.; Vigne, P.; Vijverberg, H.P.M. Polypeptide toxins as tools to study voltage-sensitive sodium channels. Ann. N. Y. Acad. Sci. 479: 204-220;1986.
4. + channels by a novel scorpion toxin. Nature 296:90-91;1982.
5. + channels from mammalian skeletal muscle. Nature 313:316-318;1985.
6. DeBin, J.A.; Maggio, J.E.; Strichartz, G.R. Purification and characterization of chlorotoxin, a chloride channel ligand from the venom of the scorpion. Cell. Physiol. 264:C361-C369;1993.
7. Valdivia, H.H.; Kirby, M.S.; Lederer, W.J.; Coronado, R. Scorpion toxins targeted against the sarcoplasmic reticulum Ca (2 +)-release channel of skeletal and cardiac muscle. Proc. Natl. Acad. Sci. USA 89:12185-12189;1992.
8. Possani, L.D. Structure of scorpion toxins. In Handbook of Natural Toxins, Vol. 2. New York: Marcel Dekker, Inc.; 1984: 513-550.
9. Kobayashi, Y.; Takashima, H.; Tamaoki, H.; Kiogoku, Y.; Lambert, P.; Kuroda, H.; Chino, N.; Watanabe, T.X.; Kimura, T.; Sakakibara, S.; Moroder, L. The Cystine-Stabilized α-Helix: A Common Structural Motif of Ion-Channel Blocking Neurotoxic Peptidcs. Biopolymers 31:1213-1220;1991.
10. Ménez, A.; Bontems, F.; Roumestand, C.; Gilquin, B.; Toma, F. Structural basis for functional diversity of animal toxins. Proc. Roy. Soc. Edinburgh 99B:83-103;1992.
11. Bontems, F.; Roumestand, C.; Gilquin, B.; Ménez, A.; Toma, F. Refined structure of Charybdotoxin: common motifs in scorpion toxins and insect defensins. Science 254:1521-1523; 1991.
12. Fontecilla-Camps, J.C.; Almassy, R.J.; Suddath, F.L.; Watt, D.D.; Bugg, C.E. Three-dimensional structure of a protein from scorpion venom: A new structural class of neurotoxins. Proc. Natl. Acad. Sci. USA 77:6496-6500;1980.
13. Lebreton, F.; Delepierre, M.; Ramírez, A.N.; Balderas, C.; Possani, L.D. Primary and NMR three-dimensional structure determination of a novel crustacean toxin from the venom of the scorpion Centruroides limpidus limpidus Karsch. Biochem. 33:11135-11149;1994.
14. Lee, W.; Moore, C.H.; Watt, D.D.; Rama Krishna, N. Solution structure of the variant-3 neurotoxin from Centruroides sculpturatus Ewing. Eur. J. Biochem. 218:89-95;1994.
15. Housset, D.; Habersetzer-Rochat, C.; Astier, J.-P.; Fontecilla-Camps J.C. Crystal structure of toxin II from the scorpion Androctonus australis Hector refined at 1.3 A resolution. J. Mol. Biol. 238:88-103;1994.
16. Darbon, H.; Weber, C.; Braun, W. Two-dimensional 1H Nuclear Magnetic Resonance study of AaH IT, an anti-insect toxin from the scorpion Androctonus australis Hector. Sequential resonance assignments and folding of the polypeptide chain. Biochem. 30:1836-1845;1991.
17. Eitan, M.; Fowler, E.; Herrmann, R.; Duval, A.; Pelhate, M.; Zlotkin, E. A scorpion venom neurotoxin paralytic to insects that affects sodium current inactivation: purification, primary structure and mode of action. Biochem. 29:5941-5947;1990.
18. De Lima, M.E.; Martin, M.F.; Diniz, C.R.; Rochat, H. Tityus serrulatus toxin VII bears pharmacological properties of both β-toxin and insect toxin from scorpion venoms. Biochem. Biophys. Research Commun. 139:296-302;1986.
19. Loret, E.P.; Martin-Eauclaire, M.-F.; Mansuelle, P.; Sampieri, F.; Granier, C.; Rochat H. An anti-insect toxin purified from the scorpion Androctonus australis Hector also acts on the α-and β-sites of the mammalian sodium channel: sequence and circular dichroism study. Biochem. 30:633-640;1991.
20. Gordon, D.; Martin-Eauclaire, M.F.; Cestèle, S.; Kopeyan, E.C.; Khalifa, R.B.; Pelhate, M.; Rochat, H. Scorpion toxins affecting sodium current inactivation bind to distinct homologous receptor sites on rat brain and insect sodium channels. J. Biol. Chem. 271:8034-8045;1996.
21. Jover, E.; Couraud, F.; Rochat, H. Two types of scorpion neurotoxins characterized by their binding to two separate receptor sites on rat brain synaptosomes. Biochem. Biophys. Res. Commun. 64:1607-1614;1980.
22. Catterall, W.A. Structure and function of voltage-gated sodium and calcium channels. Current Opinion Neurobiol. 1: 5-13;1991.
23. Wheeler, K.P.; Watt, D.D.; Lazdunski, M. Classification of Na channel receptors specific for various scorpion toxins. Pflügers Arch. 97:164-165;1983.
24. Meves, H.; Simard, J.M.; Watt, D.D. Biochemical and electrophysiological characteristics of toxins isolated from the venom of the scorpion Centruroides sculpturatus. J. Physiol., Paris 79: 185-191;1984.
25. Alagón, A.C.; Guzman, H.S.; Martin, B.M.; Ramírez, A.N.; Carbone, E.; Possani, L.D. Isolation and characterization of two toxins from the Mexican scorpion Centruroides limpidus limpidus Karsch. Comp. Biochem. Physiol. 89B:153-161; 1988.
26. Cestèle, S.; Khalifa, R.B.; Pelhate, M.; Rochat, H.; Gordon, D. α-Scorpion toxins binding on rat brain and insect sodium channels reveal divergent allosteric modulations by brevetoxin and veratridine. J. Biol. Chem. 270:15153-15161; 1995.
27. Yatani, A.; Kirsch, G.E.; Possani, L.D.; Brown, M. Effects of New World scorpion toxins on single-channel and whole cell cardiac sodium currents. Am. J. Physiol. 254:H443-H451; 1988.
28. Zlotkin, E.; Kadouri, D.; Gordon, D.; Pelhate, M.; Martin M.F.; Rochat, H. An excitatory and a depressant insect toxin from scorpion venom both affect sodium conductance and possess a common binding site. Arch. Biochem. Biophys. 240: 877-887;1985.
29. Gordon, D.; Jover, E.; Couraud, F.; Zlotkin E. The binding of the insect-selective neurotoxin (AalT) from scorpion venom to locust synaptosomal membranes. Biochim. Biophys. Acta 778:349-358;1984.
30. Zlotkin, E.; Gurevitz, M.; Fowler, E.; Adams M.E. Depressant insect selective neurotoxins from scorpion venom: chemistry, action, and gene cloning. Arch. Insect Biochem. Physiol. 22: 55-73;1993.
31. Gordon, D.; Moskowitz, H.; Eitan, M.; Warner, C.; Catterall, W.A.; Zlotkin, E. Localization of receptor sites for insect-selective toxins on sodium channels by site-directed antibodies. Biochem. 31:7622-7628;1992.
32. Possani, L.D.; Dent, M.A.R.; Martin, B.M.; Maelicke, A.; Svendsen, I. The amino terminal sequence of several toxins from the venom of the Mexican scorpion Centruoides noxius Hoffmann. Carlsberg Res. Commun. 46:207-214; 1981.
33. Reisfeld, R.A.; Lewis, V.J.; Williams, D.E. Disk electrophoresis of basic proteins and peptides on polyacrylamide gels. Nature 195:281-285;1979.
34. Laemmli, U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-685; 1970.
35. Possani, L.D.; Martin, B.M.; Svendsen, I.; Rode G.S.; Erickson, B.W. Scorpion toxins from Centruroides noxius and Tityus serrulatus. Biochem. J. 229:739-750; 1985.
36. Martin, B.M.; Carbone, E.; Yatani, A.; Brown, A.M.; Ramírez, A.N.; Gurrola, G.B.; Possani, L.D. Amino acid sequence and physiological characterization of toxins from the venom of the scorpion Centruroides limpidus tecomanus Hoffmann. Toxicon 26:785-794;1988.
37. Valdivia, H.H.; Martin, B.M.; Ramírez, A.N.; Fletcher, P.L.; Possani, L.D. Isolation and pharmacological characterization of four novel Na+ channel-blocking toxins from the scorpion Centruroides noxius Hoffmann. J. Biochem. 116:1383-1391; 1994.
38. Possani, L.D.; Martin, B.M.; Fletcher, M.D.; Fletcher, P.L. Discharge effect on pancreatic exocrine secretion produced by toxins purified from Tityus serrulatus scorpion venom. J. Biol. Chem. 266:3178-3185;1991.
39. Dehesa-DáVila, M.; Ramírez, A.N.; Zamudio, F.Z.; GurrolaBriones, G.; Liévano, A.; Darszon, A.; Possani, L.D. Structural and functional comparison of toxins from the venom of the scorpions Centruroides infamatus infamatus, Centruroides limpidus limpidus and Centruroides noxins. 113B:331-339;1996.
40. +-channel-blocking toxins of the scorpion Centruroides noxius Hoffmann. Gene 128:165-171;1993.
41. Bougis, P.E.; Rochat, H.; Smith, L.A. Precursors of Androctonus australis Hector. J. Biol. Chem. 264:19259-19265; 1989.
42. Vázquez, A.; Becerril, B.; Martin, B.M.; Zamudio, F.; Bolivar, F.; Possani, L.D. Primary structure determination and cloning of the cDNA encoding toxin 4 of the scorpion Centruroides noxius Hoffmann. FEBS Lett. 320:43-46;1993.
43. + channel-specific toxins 1 and 2 of the scorpion Centruroides noxius Hoffmann. Toxicon 33:1161-1170;1995.
44. Creighton, T.E. Proteins-Structures and Molecular Properties, 2nd edition. New York: W. H. Freeman; 1993:90.
45. Zamudio, F.; Saavedra, R.; Martin, B.M.; Gurrola-Briones, G.; Herion, P.; Possani, L.D. Amino acid sequence and immunological characterization with monoclonal antibodies of two toxins from the venom of the scorpion Centruroides noxius Hoffmann. Eur. J. Biochem. 204:281-292;1992.
46. Loret, E.P.; Sampieri, F.; Roussel, A.; Granier, C.; Rochat, H. Conformational flexibility of a scorpion toxin active on mammals and insects: a circular dichroism study. Proteins 8: 164-172;1990.
47. Fontecilla-Camps, J.C.; Almassy, R.J.; Suddath, F.L.; Bugg C.E. The three-dimensional structure of scorpion neurotoxins. Toxicon 20:1-7;1982.
48. Kharrat, R.; Darbon, H.; Rochat, H.; Granier, C. Structure/ activity relationships of scorpion α-toxins. Eur. J. Biochem. 181:381-390;1989.
49. Kharrat, R.; Darbon, H.; Granier, C.; Rochat H. Structure-activity relationships of scorpion α-neurotoxins: contributions of arginine residues. Toxicon 28:509-523;1990.
50. El-Ayeb, M.; Darbon, H.; Bahraoui, E.; Vargas, O.; Rochat, H. Differential effects of defined chemical modifications on antigenic and pharmacological activities of scorpion alpha-and beta-toxins. Eur. J. Biochem. 155:289-294;1986.
51. Jover, E.; Bablito, J.; Couraud, F. Binding of β-toxin: a physicochemical study. Biochem. 23:1147-1152;1984.
52. Darbon, H.; Angelides, K.J. Structural mapping of the voltage-dependent sodium channel. J. Biol. Chem. 259:6074-6084; 1984.
53. + channel structure from a complete functional map of the molecular surface of charybdotoxin. Biochem. 33:443-450;1994.
54. Babin, D.R.; Watt, D.D.; Goos S.M.; Mlejnek, R.V. Amino acid sequences of neurotoxic protein variants from the venom of Centruroides sculpturatus Ewing. Arch. Biochem. Biophys. 164:694-706;1974.
55. Ramírez, A.N.; Martin, B.M.; Gurrola, G.B.; Possani, L.D. Isolation and characterization of a novel toxin from the venom of the scorpion Centruroides limpidus limpidus Karsch. Toxicon 32:479-490;1994.