Rapid automated derivatization of solid-phase supports for oligonucleotide synthesis using uronium or phosphonium coupling reagents

Tetrahedron Letters

Volumn 38, Issue 19, 1997, Pages 3331-3334

  Pon, Richard T ^a   Yu, Shuyuan ^a  

^a UNIVERSITY OF CALGARY   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

OLIGONUCLEOTIDE;

ARTICLE; AUTOMATION; DRUG SYNTHESIS; IN VITRO STUDY; REACTION ANALYSIS; TECHNIQUE;

EID: 0030993211     PISSN: 00404039     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0040-4039(97)00620-5     Document Type: Article

References (27)

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24. bz, R=H, and DIEA (1:1, 0.05M) and a solution of coupling reagent (0.05M) to the synthesis column (4.0 sec); 3, flushed and rinsed the column with Ar and acetonitrile (3x); and 4, primed the phosphoramidite and tetrazole lines. The support (∼ 12 mg) was accurately weighed into the synthesis column, so nucleoside loadings could be determined from the trityl color released from the first detritylation. Immediately after completion of the "Begin" procedure, an unmodified oligonucleotide synthesis program began.
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27. There are sufficient base positions on an eight port PE/ABD 394 to allow automated derivatization of up to three nucleosides per synthesizer and facilities with at least two synthesizers could use this method, if software to deliver different bases to different columns was available. Otherwise with existing software, each column must begin with the same nucleoside.