Quantification of vitamin D receptor mRNA in tissue sections demonstrates the relative limitations of in situ-reverse transcriptase- polymerase chain reaction

Journal of Pathology

Volumn 182, Issue 1, 1997, Pages 22-28

  Mee, Andrew P ^a   Denton, John ^b   Hoyland, Judith A ^b   Davies, Michael ^a   Mawer, E Barbara ^a  

^a MANCHESTER ROYAL INFIRMARY   (United Kingdom)

^b UNIVERSITY OF MANCHESTER   (United Kingdom)

Author keywords

bone; image analysis; in situ hybridization; in situ reverse transcriptase polymerase chain reaction; kidney; quantitative autoradiography; vitamin D receptor

Indexed keywords

CALCITRIOL; MESSENGER RNA; VITAMIN D RECEPTOR;

ARTICLE; HUMAN; HUMAN CELL; HUMAN TISSUE; IMAGE ANALYSIS; PRIORITY JOURNAL; QUANTITATIVE DIAGNOSIS; RECEPTOR GENE; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA ANALYSIS; SIGNAL TRANSDUCTION; VITAMIN METABOLISM;

BONE AND BONES; HUMANS; HYPERPARATHYROIDISM, SECONDARY; IMAGE PROCESSING, COMPUTER-ASSISTED; IN SITU HYBRIDIZATION; KIDNEY; OSTEITIS DEFORMANS; POLYMERASE CHAIN REACTION; RECEPTORS, CALCITRIOL; RNA, MESSENGER;

EID: 0030992203     PISSN: 00223417     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1096-9896(199705)182:1<22::AID-PATH809>3.0.CO;2-I     Document Type: Article

References (22)

1. Komminoth P, Long A. In-situ polymerase chain reaction. An overview of methods, applications and limitations of a new molecular technique. Virchows Arch B 1993; 64: 67-73.
2. Chen RH, Fuggle SV. In situ cDNA polymerase chain reaction. A novel technique for detecting mRNA expression. Am J Pathol 1993; 143: 1527-1534.
3. Embleton MJ, Gorochov G, Jones PT, Winter G. In-cell PCR from mRNA: amplifying and linking the rearranged immunoglobulin heavy and light chain V-genes within single cells. Nucleic Acids Res 1992; 20: 3831-3837.
4. Heniford BW, Shum-Siu A, Leonberger M, Hendler FJ. Variation in cellular EGF receptor mRNA expression demonstrated by in situ reverse transcriptase polymerase chain reaction. Nucleic Acids Res 1993; 21: 3159-3166.
5. Martinez A, Miller MJ, Quinn K, Unsworth EJ, Ebina M, Cuttita F. Non-radioactive localisation of nucleic acids by direct in situ-PCR and in situ RT-PCR in paraffin-embedded sections. J Histochem Cytochem 1995; 43: 739-747.
6. Mee AP, Davenport LK, Hoyland JA, Davies M, Mawer EB. Novel detection systems for the vitamin D receptor - in situ-reverse transcriptase-polymerase chain reaction and immunogold cytochemistry. J Mol Endocrinol 1996; 16: 183-195.
7. Mee AP, Hoyland JA, Braidman IP, Freemont AJ, Davies M, Mawer EB. Demonstration of vitamin D receptor transcripts in actively resorbing osteoclasts in bone sections. Bone 1996; 18: 295-299.
8. 3 receptors: gene regulation and genetic circuitry. FASEB J 1988; 2: 3043-3053.
9. 3 receptors: structure and function in transcription. Annu Rev Nutr 1991; 11: 189-216.
10. Rodan GA, Martin TJ. Role of osteoblasts in hormonal control of bone resorption - a hypothesis. Calcif Tissue Int 1981; 33: 349-351.
11. Komminoth P, Long A, Ray R, Wolfe H. In situ polymerase chain reaction detection of viral DNA, single copy genes and gene rearrangements in cell suspensions and cytospins. Diagn Mol Pathol 1992; 1: 85 97.
12. 33P. J Histochem Cytochem 1993; 41: 1767-1776.
13. 2 receptor and glutamic acid decarboxylase mRNAs in mouse substantia nigra. Eur J Pharmacol 1994; 269: 25-33.
14. Westlake TM, Howlett AC, Bonner TI, Matsuda LA, Herkenham M. Cannabinoid receptor binding and messenger RNA expression in human brain: an in vitro receptor autoradiography and in situ hybridisation histochemistry study of normal aged and Alzheimer's brains, Neuroscience 1994; 63: 637-652.
15. Fraser HM, Lunn SF, Whitelaw PF, Hillier SG. Induced luteal regression: differential effects on follicular and luteal inhibin/activin subunit mRNAs in the marmoset monkey. J Endocrinol 1995; 144: 201-208.
16. Kordylewski L, Ambler SK, Doyle DD. Quantification of ANP mRNA in primary cultures of adult atrial myocytes by image processing: in situ hybridisation to multiple parallel samples using single-stranded cDNA probes. J Histochem Cytochem 1995; 43: 481-488.
17. A-subunits in the fetal sheep testis. J Endocrinol 1995; 145: 35-42.
18. Birek C, Lui E, Dardick. c-fos oncogene underexpression in salivary gland tumours as measured by in situ hybridisation. Am J Pathol 1993; 142: 917-923.
19. Lucas LR, Mize RR, Harlan R. Semiquantitative analysis of in situ hybridisation results using IMAGE software: a rapid method for counting reduced silver grains over mRNA-positive cells. J Neurosci Methods 1994; 52: 101-109.
20. Masseroli M, Bollea A, Bendotti C, Forloni G. In situ hybridisation histochemistry quantification: automatic count on single cell in digital image. J Neurosci Methods 1993; 47: 93-103.
21. Rentrop M, Knapp B, Winter H, Schweizer J. Aminoalkylsilane-treated slides as support for in situ hybridisation of keratin cDNAs to frozen sections under varying fixation and pretreatment conditions. Histochem J 1986; 18: 271-276.
22. Baker AR, McDonnell DP, Hughes M, et al. Cloning and expression of full-length cDNA encoding human vitamin D receptor. Proc Natl Acad Sci USA 1988; 85: 3294-3298.