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Muller, A.M.1
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Godin I, Dieterlen-Lievre F, Cumano A. Emergence of multipotent hemopoietic cells in the yolk sac and paraortic splanchnopleura in mouse embryos, beginning at 8.5 days postcoitus. Proc Natl Acad Sci USA. 92:1995;773-777.
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Lymphoid potential, probed before circulation in mouse, is restricted to caudal intraembryonic splanchnopleura
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of outstanding interest. The splanchnopleural mesoderm and yolk sac were dissected from embryos before the onset of circulation and were cultured. The splanchnopleural mesoderm gave rise to multipotent progenitor activity, whereas the yolk sac only gave rise to progenitors that exhibited restricted myeloid progenitor activity. Multipotent progenitor activity was only observed in the yolk sac after the onset of circulation, suggesting that the yolk sac is seeded by progenitors that form in the splanchnopleural mesoderm.
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of outstanding interest Cumano A, Dieterlen-Lievre F, Godin I. Lymphoid potential, probed before circulation in mouse, is restricted to caudal intraembryonic splanchnopleura. Cell. 86:1996;907-916 The splanchnopleural mesoderm and yolk sac were dissected from embryos before the onset of circulation and were cultured. The splanchnopleural mesoderm gave rise to multipotent progenitor activity, whereas the yolk sac only gave rise to progenitors that exhibited restricted myeloid progenitor activity. Multipotent progenitor activity was only observed in the yolk sac after the onset of circulation, suggesting that the yolk sac is seeded by progenitors that form in the splanchnopleural mesoderm.
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Cumano, A.1
Dieterlen-Lievre, F.2
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16
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Definitive hematopoiesis is autonomously initiated by the AGM region
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of outstanding interest. This group reports complementary findings to those in [15] from mice at a somewhat later stage of development. The splanchnopleural mesoderm described in [15] gives rise to the aorta-gonad-mesonephros (AGM) described in [16]. This paper demonstrates long-term HSC activity in the AGM, but not the yolk sac, soon after the onset of circulation. Together the observations of these two groups [15,16] suggest that definitive HSCs form de novo only from the dorsal mesoderm; however, given previous inconsistent results, these results must be confirmed by other approaches.
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of outstanding interest Medvinsky A, Dzierzak E. Definitive hematopoiesis is autonomously initiated by the AGM region. Cell. 86:1996;897-906 This group reports complementary findings to those in [15] from mice at a somewhat later stage of development. The splanchnopleural mesoderm described in [15] gives rise to the aorta-gonad-mesonephros (AGM) described in [16]. This paper demonstrates long-term HSC activity in the AGM, but not the yolk sac, soon after the onset of circulation. Together the observations of these two groups [15,16] suggest that definitive HSCs form de novo only from the dorsal mesoderm; however, given previous inconsistent results, these results must be confirmed by other approaches.
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Medvinsky, A.1
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of special interest. An excellent embryological review of the formation of the hematopoietic system, with a perspective that includes amphibians, birds, and mammals. Evidence is reviewed demonstrating that the relative roles of the dorsal and ventral mesoderm in early hematopoiesis varies between species. The review includes a summary of recent advances dissecting the genetics of HSC formation and provides insight into which model systems will yield future advances.
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of special interest Zon LI. Developmental biology of hematopoiesis. Blood. 86:1995;2876-2891 An excellent embryological review of the formation of the hematopoietic system, with a perspective that includes amphibians, birds, and mammals. Evidence is reviewed demonstrating that the relative roles of the dorsal and ventral mesoderm in early hematopoiesis varies between species. The review includes a summary of recent advances dissecting the genetics of HSC formation and provides insight into which model systems will yield future advances.
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Zon, L.I.1
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Tavassoli, M.1
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19
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0030581174
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The T cell leukemia oncoprotein SCL/tal-1 is essential for development of all hematopoietic lineages
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of special interest. SCL is the only gene that has been shown to be required for all hematopoiesis. Deficiencies in other genes have been shown to severely disrupt, but not to prevent, all hematopoiesis. SCL may be required for the formation of HSCs.
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of special interest Porcher C, Swat W, Rockwell K, Fujiwara Y, Alt FW, Orkin SH. The T cell leukemia oncoprotein SCL/tal-1 is essential for development of all hematopoietic lineages. Cell. 86:1996;47-57 SCL is the only gene that has been shown to be required for all hematopoiesis. Deficiencies in other genes have been shown to severely disrupt, but not to prevent, all hematopoiesis. SCL may be required for the formation of HSCs.
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Porcher, C.1
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20
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AML1, the target of multiple chromosomal translocations in human leukemia, is essential for normal fetal liver hematopoiesis
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Okuda, T.1
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22
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0028907847
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Murine hematopoietic stem and progenitor cells. I. Enrichment and biologic characterization
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lo cells. In addition to reproducing the high radioprotective activity of these cells, this paper shows that 80% of the cells formed colonies in methylcellulose in response to a combination of cytokines but that few colonies were formed in response to single cytokines that stimulate more differentiated progenitor cells.
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lo cells. In addition to reproducing the high radioprotective activity of these cells, this paper shows that 80% of the cells formed colonies in methylcellulose in response to a combination of cytokines but that few colonies were formed in response to single cytokines that stimulate more differentiated progenitor cells.
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Li, C.L.1
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23
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0028908916
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Long-term repopulation of irradiated mice with limiting numbers of purified hematopoietic stem cells: In vivo expansion of stem cell phenotype but not function
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-/lo cells that had a low level of Rhodamine 123. These cells were highly radioprotective. Together with the data from [22], the data from this study suggest that a maximum of one out of five intravenously injected cells is able to home to hematopoietic tissues and give rise to detectable numbers of progeny.
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-/lo cells that had a low level of Rhodamine 123. These cells were highly radioprotective. Together with the data from [22], the data from this study suggest that a maximum of one out of five intravenously injected cells is able to home to hematopoietic tissues and give rise to detectable numbers of progeny.
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Blood
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Spangrude, G.J.1
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Tumas, D.B.3
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24
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8944226104
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Characterization of mouse lymphohematopoietic stem cells lacking spleen colony-forming activity
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of special interest. The authors purified a population of long-term reconstituting HSCs that had neither CFU-S nor radioprotective activity. These differences relative to previous papers [4,8,22,23] may be explained by the more exhaustive ablation of the hematopoietic systems of recipient mice by the administration of a cytotoxic drug followed by irradiation, or by the use of a different strain of donor mouse (DBA/2×C57BL/6 F1 mice versus C57BL/6 used by other groups). The HSC population purified by Jones et al. was also reported to lack the expression of surface markers shown by other groups to be expressed in all C57BL/6 HSCs.
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of special interest Jones RJ, Collector MI, Barber JP, Vala MS, Fackler MJ, May WS, Griffin CA, Hawkins AL, Zehnbauer BA, Hilton J, et al. Characterization of mouse lymphohematopoietic stem cells lacking spleen colony-forming activity. Blood. 88:1996;487-491 The authors purified a population of long-term reconstituting HSCs that had neither CFU-S nor radioprotective activity. These differences relative to previous papers [4,8,22,23] may be explained by the more exhaustive ablation of the hematopoietic systems of recipient mice by the administration of a cytotoxic drug followed by irradiation, or by the use of a different strain of donor mouse (DBA/2×C57BL/6 F1 mice versus C57BL/6 used by other groups). The HSC population purified by Jones et al. was also reported to lack the expression of surface markers shown by other groups to be expressed in all C57BL/6 HSCs.
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Blood
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Jones, R.J.1
Collector, M.I.2
Barber, J.P.3
Vala, M.S.4
Fackler, M.J.5
May, W.S.6
Griffin, C.A.7
Hawkins, A.L.8
Zehnbauer, B.A.9
Hilton, J.10
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25
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0029796633
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Long-term lymphohematopoietic reconstitution by a single CD34-low/negative hematopoietic stem cell
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lo cells also contained long-term HSCs, but were not assayed for radioprotection. Again, the conditions for hematopoietic ablation of recipient mice in radioprotection assays were more stringent than those used in previous studies reporting that long-term HSCs were radioprotective.
-
lo cells also contained long-term HSCs, but were not assayed for radioprotection. Again, the conditions for hematopoietic ablation of recipient mice in radioprotection assays were more stringent than those used in previous studies reporting that long-term HSCs were radioprotective.
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Science
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Osawa, M.1
Hanada, K.-I.2
Hamada, H.3
Nakauchi, H.4
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26
-
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0029813378
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The aging of hematopoietic stem cells
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of special interest. Although old and young bone marrow have similar progenitor activities, HSCs were seen to change in several ways as mice age. Most notably, they increase in number and are driven into cycle in old mice. By comparing the activities of HSCs in four different in vivo and in vitro assays of multipotent progenitor activity, this paper provides evidence that the efficiency with which HSCs engraft irradiated mice after intravenous injection can vary depending on donor age.
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of special interest Morrison SJ, Wandycz AM, Akashi K, Globerson A, Weissman IL. The aging of hematopoietic stem cells. Nat Med. 2:1996;1011-1016 Although old and young bone marrow have similar progenitor activities, HSCs were seen to change in several ways as mice age. Most notably, they increase in number and are driven into cycle in old mice. By comparing the activities of HSCs in four different in vivo and in vitro assays of multipotent progenitor activity, this paper provides evidence that the efficiency with which HSCs engraft irradiated mice after intravenous injection can vary depending on donor age.
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(1996)
Nat Med
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Morrison, S.J.1
Wandycz, A.M.2
Akashi, K.3
Globerson, A.4
Weissman, I.L.5
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27
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85030298173
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Cyclophosphamide/G-CSF induces hematopoietic stem cells to proliferate prior to mobilization
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in press
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Morrison SJ, Wright DE, Weissman IL. Cyclophosphamide/G-CSF induces hematopoietic stem cells to proliferate prior to mobilization. Proc Natl Acad Sci USA. 1997;. in press.
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Proc Natl Acad Sci USA
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Morrison, S.J.1
Wright, D.E.2
Weissman, I.L.3
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28
-
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0027855043
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In vivo and in vitro characterization of long-term repopulating primitive hematopoietic cells isolated by sequential Hoechst 33342-rhodamine 123 FACS selection
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Wolf NS, Kone A, Priestley GV, Bartelmez SH. In vivo and in vitro characterization of long-term repopulating primitive hematopoietic cells isolated by sequential Hoechst 33342-rhodamine 123 FACS selection. Exp Hematol. 21:1993;614-622.
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Wolf, N.S.1
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Bartelmez, S.H.4
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29
-
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0029917125
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Genetic control of the frequency of hematopoietic stem cells in mice: Mapping of a candidate locus to chromosome 1
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of special interest. Two loci on chromosome 1 were found to correlate with multipotent progenitor frequency in mice. This is the first report of efforts to systematically identify genes that regulate HSC number. Because such genes presumably include factors that regulate self-renewal and survival, identification of these genes may advance efforts to expand HSCs ex vivo.
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of special interest Muller-Sieburg CE, Riblet R. Genetic control of the frequency of hematopoietic stem cells in mice: mapping of a candidate locus to chromosome 1. J Exp Med. 183:1996;1141-1150 Two loci on chromosome 1 were found to correlate with multipotent progenitor frequency in mice. This is the first report of efforts to systematically identify genes that regulate HSC number. Because such genes presumably include factors that regulate self-renewal and survival, identification of these genes may advance efforts to expand HSCs ex vivo.
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J Exp Med
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Muller-Sieburg, C.E.1
Riblet, R.2
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30
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31
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13344269014
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Hematopoietic defects in mice lacking the sialomucin CD34
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Cheng J, Baumhueter S, Cacalano G, Carver-Moore K, Thibodeaux H, Thomas R, aiBroxmeyer HE, Cooper S, Hague N, Moore M, Lasky LA. Hematopoietic defects in mice lacking the sialomucin CD34. Blood. 87:1996;479-490.
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Cheng, J.1
Baumhueter, S.2
Cacalano, G.3
Carver-Moore, K.4
Thibodeaux, H.5
Thomas, R.6
Aibroxmeyer, H.E.7
Cooper, S.8
Hague, N.9
Moore, M.10
Lasky, L.A.11
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32
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15844403221
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CD34-deficient mice have reduced eosinophil accumulation after allergen exposure and show a novel crossreactive 90 kD protein
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Suzuki A, Andrew DP, Gonzalo J-A, Fukumoto M, Spellberg J, Hashiyama M, Takimoto H, Gerwin N, Webb I, Molineux G, et al. CD34-deficient mice have reduced eosinophil accumulation after allergen exposure and show a novel crossreactive 90 kD protein. Blood. 87:1996;3550-3562.
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Suzuki, A.1
Andrew, D.P.2
Gonzalo, J.-A.3
Fukumoto, M.4
Spellberg, J.5
Hashiyama, M.6
Takimoto, H.7
Gerwin, N.8
Webb, I.9
Molineux, G.10
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33
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0028200101
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Characterization of murine CD34, a marker for hematopoietic progenitor and stem cells
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Krause DS, Ito T, Fackler MJ, Smith OM, Collector MI, Sharkis SJ, May WS. Characterization of murine CD34, a marker for hematopoietic progenitor and stem cells. Blood. 84:1994;691-701.
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Krause, D.S.1
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Sharkis, S.J.6
May, W.S.7
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34
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0029850531
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Morel F, Szilvassy SJ, Travis M, Chen B, Galy A. Primitive hematopoietic cells in murine bone marrow express the CD34 antigen. Blood. 88:1996;3774-3784.
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Morel, F.1
Szilvassy, S.J.2
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36
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0030007289
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Expression of murine CD38 defined a population of long-term reconstituting hematopoietic stem cells
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Randall TD, Lund FE, Howard MC, Weissman IL. Expression of murine CD38 defined a population of long-term reconstituting hematopoietic stem cells. Blood. 87:1996;4057-4067.
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Randall, T.D.1
Lund, F.E.2
Howard, M.C.3
Weissman, I.L.4
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38
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0028077099
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Evidence for a mitotic clock in human hematopoietic stem cells: Loss of telomeric DNA with age
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Vaziri H, Dragowska W, Allsopp RC, Thomas TE, Harley CB, Lansdorp PM. Evidence for a mitotic clock in human hematopoietic stem cells: loss of telomeric DNA with age. Proc Natl Acad Sci USA. 91:1994;9857-9860.
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Vaziri, H.1
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Harley, C.B.5
Lansdorp, P.M.6
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39
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0030248792
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Telomerase activity in hematopoietic cells is associated with self-renewal potential
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of special interest. Telomere length has been proposed to act as a mitotic clock, regulating the proliferative potential of cells. Telomerase activity has been widely observed in immortal cells, but infrequently in normal somatic cells. This study reports that self-renewing but not non-self-renewing mouse hematopoietic cells, irrespective of differentiation state, expressed telomerase. In particular most HSCs but few non-self-renewing multipotent progenitors expressed telomerase. Thus the developmental characteristic most consistently associated with telomerase expression is self-renewal potential. Telomerase may be a conserved element of genetic programs to effect self-renewing divisions.
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of special interest Morrison S, Prowse KR, Ho P, Weissman I. Telomerase activity in hematopoietic cells is associated with self-renewal potential. Immunity. 5:1996;207-216 Telomere length has been proposed to act as a mitotic clock, regulating the proliferative potential of cells. Telomerase activity has been widely observed in immortal cells, but infrequently in normal somatic cells. This study reports that self-renewing but not non-self-renewing mouse hematopoietic cells, irrespective of differentiation state, expressed telomerase. In particular most HSCs but few non-self-renewing multipotent progenitors expressed telomerase. Thus the developmental characteristic most consistently associated with telomerase expression is self-renewal potential. Telomerase may be a conserved element of genetic programs to effect self-renewing divisions.
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(1996)
Immunity
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