Use of an enzymatic micromethod to quantify amastigote stage of Leishmania amazonensis in vitro

Parasitology Research

Volumn 83, Issue 4, 1997, Pages 401-403

  Sereno, Denis ^a   Lemesre, Jean Loup ^a  

^a INSTITUT DE RECHERCHE POUR LE DÉVELOPPEMENT   (France)

Author keywords

[No Author keywords available]

Indexed keywords

PENTAMIDINE;

AMASTIGOTE; ANIMAL EXPERIMENT; ARTICLE; CONTROLLED STUDY; IN VITRO STUDY; LEISHMANIA; NONHUMAN; PARASITE CULTIVATION; PARASITE DEVELOPMENT; PRIORITY JOURNAL; QUANTITATIVE ASSAY;

ANIMALS; DOSE-RESPONSE RELATIONSHIP, DRUG; LEISHMANIA; PARASITOLOGY; PENTAMIDINE; TETRAZOLIUM SALTS; THIAZOLES; TRYPANOCIDAL AGENTS;

ANIMALIA; LEISHMANIA AMAZONENSIS;

EID: 0030990411     PISSN: 00443255     EISSN: None     Source Type: Journal    
DOI: 10.1007/s004360050272     Document Type: Article

References (10)

1. Bates PA (1993) Axenic culture of Leishmania amastigotes. Parasitol Today 9: 143-146
2. Berg K, Zhai L, Chen M, Kharazmi A, Owen TC (1994) The use of a water-soluble formazan complex to quantitate the cell number and mitochondrial function of Leishmania major promastigotes. Parasitol Res 80: 235-239
3. Berman JD, Wyler DJ (1980) An in vitro model for investigation of chemotherapeutic agents in leishmaniasis. J Infect Dis 142: 83-86
4. Cole SPC (1986) Rapid chemosensitivity testing of human lung tumor cells using the MTT assay. Cancer Chemother Pharmacol 17: 259-263
5. Lemesre JL, Darcy F, Capron A, Santoro F (1988) Requirements of defined cultivation conditions for standard growth of Leishmania promastigotes in vitro. Acta Trop (Basel) 45: 99-108
6. Lemesre JL, Blanc MP, Grebaut P, Zilberfarb V, Carriere V (1994) Culture continue des formes amastigotes de leishmanies en condition axénique. Réalisation du cycle évolutif in vitro. Med Armees 22: 99
7. Marr JJ (1980) Carbohydrate metabolism in Leishmania. In: Levandowsky M, Hunter SH (eds) Physiology and biochemistry of protozoa vol 3. Academic Press, New York, pp 171-210
8. Mosmann T (1983) Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytoxicity. J Immunol Methods 65: 55-63
9. Mukkada AJ, Meade JC, Glaser TA, Bonventre PF (1985) Enhanced metabolism of Leishmania donovani amastigotes at acidic pH: an adaptation for intracellular growth. Science 229: 1099-1101
10. Rabinovitch M, Zilberfarb V, Ramazeilles C (1986) Destruction of Leishmania mexicana amazonensis amastigotes within macrophage by lysosomotropic amino acid esters. J Exp Med 163: 520-535