In situ Trypan Blue staining of monolayer cell cultures for permanent fixation and mounting

BioTechniques

Volumn 22, Issue 6, 1997, Pages 1020-1024

  Perry, Seth W ^a   Epstein, Leon G ^a   Gelbard, Harris A ^a  

^a UNIVERSITY OF ROCHESTER MEDICAL CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

TRYPAN BLUE;

ARTICLE; CELL ADHESION; CELL CULTURE; CELL VIABILITY; CYTOTOXICITY; NERVE CELL;

EID: 0030989257     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/97226bm01     Document Type: Article

References (6)

1. Allison, D.C. and P. Ridolpho. 1980. Use of a Trypan Blue assay to measure the deoxyribonucleic acid content and radioactive labeling of viable cells. J. Histochem. Cytochem. 28:700-703.
2. Gelbard, H.A., H.S.L.M. Nottet, S. Swindells, M. Jett, K.A. Dzenko, P. Genis, R. White, L. Wang et al. 1994. Platelet-activating factor: a candidate Human Immunodeficiency Virus type 1-induced neurotoxin. J. Virol. 68:4628-4635.
3. Krause, A.W., W.W. Carley and W.W. Webb. 1984. Fluorescent Erythrosin B is preferable to Trypan Blue as a vital exclusion dye for mammalian cells in monolayer culture. J. Histochem. Cytochem. 52:1084-1090.
4. Pappenheimer, A.M. 1917. Experimental studies upon lymphocytes: I. The reactions of lymphocytes under various experimental conditions. J. Exp. Med. 25:633.
5. Patterson, M.K. 1979. Measurement of growth and viability of cells in culture. Methods Enzymol. 58:141.
6. Phillips, H.J. 1973. Dye exclusion tests for cell viability, p. 406-408. In P.F. Kruse Jr. and M.K. Patterson Jr. (Eds.), Tissue Culture: Methods and Applications. Academic Press, New York.