Transcriptional control of genes encoding CS1 Pili: Negative regulation by a silencer and positive regulation by Rns

Journal of Bacteriology

Volumn 179, Issue 18, 1997, Pages 5736-5743

  Murphree, Denise ^a   Froehlich, Barbara ^a   Scott, June R ^a  

^a EMORY UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DNA BINDING PROTEIN; ESCHERICHIA COLI ENTEROTOXIN; REPRESSOR PROTEIN;

ARTICLE; BACTERIAL GENE; BACTERIUM ADHERENCE; BACTERIUM PILUS; CONTROLLED STUDY; ESCHERICHIA COLI; GENE CONTROL; GENE EXPRESSION REGULATION; NONHUMAN; OPEN READING FRAME; OPERON; PRIORITY JOURNAL; PROMOTER REGION; SILENCER GENE; TRANSCRIPTION REGULATION; TRANSLATION INITIATION;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 0030983975     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/jb.179.18.5736-5743.1997     Document Type: Article

References (54)

1. Black, R. E. 1993. Persistent diarrhea in children of developing countries. Pediatr. Infect. Dis. J. 12:751-761.
2. Boylan, M., and C. J. Smyth. 1985. Mobilization of CS fimbriae-associated plasmids of enterotoxigenic Escherichia coli of serotype O6:K15:H16 or H-into various wild-type hosts. FEMS Microbiol. Lett. 29:83-89.
3. Busby, S., and R. H. Ebright. 1994. Promoter, structure, promoter recognition, and transcription activation in prokaryotes. Cell 79:743-746.
4. Caron, J., L. M. Coffield, and J. R. Scott. 1989. A plasmid-encoded regulatory gene, rns, required for expression of the CS1 and CS2 adhesins of enterotoxigenic Escherichia coli. Proc. Natl. Acad. Sci. USA 86:963-967.
5. Caron, J., D. R. Maneval, J. B. Kaper, and J. R. Scott. 1990. Association of rns homologs with colonization factor antigens in clinical Escherichia coli isolates. Infect. Immun. 58:3442-3444.
6. Casadaban, M. J. 1976. Transposition and fusion of the lac genes to selected promoters in Escherichia coli using bacteriophage lambda and mu. Mol. Microbiol. 104:541-555.
7. Cornelis, G., C. Sluiters. C. Lambert de Rouvroit, and T. Michiels. 1989. Homology between VirF, the transcriptional activator of the Yersinia virulence regulon, and AraC, the Escherichia coli arabinose operon regulator. J. Bacteriol. 171:254-262.
8. de Haan, L. A. M., G. A. Willshaw, B. A. M. Van Der Zegst, and W. Gaastra. 1991. The nucleotide sequence of a regulatory gene present on a plasmid in an enterotoxigenic Escherichia coli strain of serotype O167:H5. FEMS Microbiol. Lett. 83:341-346.
9. Dong, H., L. Nilsson, and C. G. Kurland. 1995. Gratuitous overexpression of genes in Escherichia coli leads to growth inhibition and ribosome destruction. J. Bacteriol. 177:1497-1504.
10. Fellay, R., J. Frey, and H. Krisch. 1987. Interposon mutagenesis of soil and water bacteria: a family of DNA fragments designed for in vitro insertional mutagenesis of gram-negative bacteria. Gene 52:147-154.
11. Fletcher, S. A., and L. N. Csonka. 1995. Fine-structure deletion analysis of the transcriptional silencer of the proU operon of Salmonella typhimurium. J. Bacteriol. 177:4508-4513.
12. Froehlich, B. J., L. Husmann, J. Caron, and J. R. Scott. 1994. Regulation of rns, a positive regulatory factor of pili of enterotoxigenic Escherichia coli. J. Bacteriol. 176:5385-5392.
13. Froehlich, B. J., A. Karakashian, L. R. Melsen, J. C. Wakefield, and J. R. Scott. 1994. CooC and CooD are required for assembly of CS1 pili. Mol. Microbiol. 12:387-401.
14. Froehlich, B. J., A. Karakashian, H. Sakellaris, and J. R. Scott. 1995. Genes for CS2 pili of enterotoxigenic Escherichia coli and their interchangeability with those for CS1 pili. Infect. Immun. 63:4849-4856.
15. Froehlich, B. J., and J. R. Scott. 1991. A single promoter-cloning vector for use in Escherichia coli. Gene 108:99-101.
16. Gaastra, W., and A.-M. Svennerholm. 1996. Colonization factors of human enterotoxigenic Escherichia coli (ETEC). Trends Microbiol. 4:444-452.
17. Goransson, M., B. Sonden, P. Nilsson, B. Dagberg, K. Forsman, K. Emanuelsson, and B. E. Uhlin. 1990. Transcriptional silencing and thermoregulation of gene expression in Escherichia coli. Nature 344:682-685.
18. Gralla, J. D., and J. Collado-Vides. 1996. Organization and function of transcription regulatory elements, p. 1232-1245. In F. C. Niedhardt (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed. ASM Press, Washington, D.C.
19. Hawley, D. K., and W. McClure. 1983. Compilation and analysis of Escherichia coli promoter DNA sequences. Nucleic Acids Res. 11:2237-2255.
20. Jordi, B. J. A. M., B. Dagberg, L. A. M. de Haan, A. M. Hamers, B. A. M. van der Zeijst, W. Gaastra, and B. E. Uhlin. 1992. The positive regulator CfaD overcomes the repression mediated by histone-like protein H-NS (H1) in the CFA/I fimbrial operon of Escherichia coli. EMBO J. 11:2627-2632.
21. Jordi, B. J. A. M., B. A. M. van der Zeijst, and W. Gaastra. 1994. Regions of the CFA/I promoter involved in the activation by transcriptional activator CfaD and repression by the histone-like protein H-NS. Biochimie 76:1052-1054.
22. Jordi, B. J. A. M., A. H. M. Van Vliet, G. A. Willshaw, B. A. M. Van Der Zeijst, and W. Gaastra. 1991. Analysis of the first two genes of the CS1 fimbrial operon in human enterotoxigenic Escherichia coli of serotype 0139: H28. FEMS Microbiol. Lett. 80:265-270.
23. Jordi, B. J. A. M., G. A. Willshaw, B. A. M. van der Zeist, and W. Gaastra. 1992. The complete nucleotide sequence of region 1 of the CFA/I fimbrial operon of human enterotoxigenic Escherichia coli. DNA Sequence 2:257-263.
24. Jubete, Y., J. C. Zabala, A. Juarez, and F. de la Cruz. 1995. hlyM, a transcriptional silencer downstream of the promoter in the hly operon of Escherichia coli. J. Bacteriol. 177:242-246.
25. Kawula, T. Personal communication.
26. Kawula, T. H., and M. J. Lelivelt. 1994. Mutations in a gene encoding a new Hsp70 suppress rapid DNA inversion and bgl activation, but not proU derepression, in hns-1 mutant Escherichia coli. J. Bacteriol. 176:610-619.
27. Kawula, T. H., and P. E. Orndorff. 1991. Rapid site-specific DNA inversion in Escherichia coli mutants lacking the histonelike protein H-NS. J. Bacteriol. 173:4116-4123.
28. Levine, M. M. 1987. Escherichia coli that cause diarrhea: enterotoxigenic, enteropathogenic, enteroinvasive, enterohemorrhagic, and enteroadherent. J. Infect. Dis. 155:377-389.
29. Lucht, J. M., P. Dersch, B. Kempf, and E. Bremer. 1994. Interactions of the nucleoid-associated DNA-binding protein H-NS with the regulatory region of the osmotically controlled proU operon of Escherichia coli. J. Biol. Chem. 269:6578-6586.
30. Maniatis, T., E. F. Fritsch, and J. Sambrook. 1982. Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
31. McClure, W. R. 1985. Mechanism and control of transcription initiation in prokaryotes. Annu. Rev. Biochem. 54:171-204.
32. Miller, J. H. 1972. Experiments in molecular genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
33. Nataro, J. P., D. Yikang, D. Yingkang, and K. Walker. 1994. AggR, a transcriptional activator of aggregative adherence fimbria 1 expression in enteroaggregative Escherichia coli. J. Bacteriol. 176:4691-4699.
34. Ogierman, M. A., and P. A. Manning. 1992. Homology of TcpN, a putative regulatory protein of Vibrio cholerae, to the AraC family of transcriptional activators. Gene 116:93-97.
35. Orndorff P. E., P. A. Spears, D. Schauer, and S. Falkow. 1985. Two modes of control of pilA, the gene encoding type 1 pilin in Escherichia coli. J. Bacteriol. 164:321-330.
36. Overdier, D. G., and L. N. Csonka. 1992. A transcriptional silencer downstream of the promoter in the osmotically controlled proU operon of Salmonella typhimurium. Proc. Natl. Acad. Sci. USA 89:3140-3144.
37. Perez-Casal, J., J. S. Swartley, and J. R. Scott. 1990. Gene encoding the major subunit of CS1 pili of human enterotoxigenic Escherichia coli. Infect. Immun. 58:3594-3600.
38. Pribnow, D. 1979. Genetic control signals in DNA., p. 219-227. In R. F. Goldberger (ed.), Biological regulation and development, vol. 1. Plenum Press, New York, N.Y.
39. Ross, W., K. K. Gosnik, J. Salomon, K. Igarashi, C. Zou, A. Ishihama, K. Severino, and R. L. Gourse. 1993. A third recognition element in bacterial promoters: DNA binding by the alpha subunit of RNA polymerase. Science 262:1407-1413.
40. Sakai, T., C. Sasakawa, S. Makino, and M. Yoshikawa. 1986. DNA sequence and prooduct analysis of the virF locus responsible for Congo red binding and cell invasion in Shigella flexneri 2a. Infect. Immun. 54:395-402.
41. Sakellaris, H., D. P. Balding, and J. R. Scott. 1996. Assembly proteins of CS1 pili of enterotoxigenic Escherichia coli. Mol. Microbiol. 21:529-541.
42. Sakellaris, H. Unpublished results.
43. Sanger, F., S. Nicklen, and A. R. Coulson. 1977. DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74:5463-5467.
44. Schnetz, K. 1995. Silencing of Escherichia coli bg promoter by flanking sequence elements. EMBO J. 14:2545-2450.
45. Scott, J. R. 1974. A turbid plaque-forming mutant of phage P1 that cannot lysogenize Escherichia coli. Virology 62:344-349.
46. Scott, J. R., J. C. Wakefield, P. W. Russell, P. E. Orndorff, and B. J. Froehlich. 1992. CooB is required for assembly but not transport of CS1 pilin. Mol. Microbiol. 6:293-300.
47. Smyth, C. J., M. B. Marron, J. M. G. J. Twohig, and S. G. J. Smith. 1996. Fimbrial adhesins: similarities and variations in structure and biogenesis. FEMS Immunol. Med. Microbiol. 16:127-139.
48. Swartley, J. Unpublished results.
49. Takeshita, S., M. Sato, M. Toba, W. Masahashi, and T. Hashimoto-Gotoh. 1987. High-copy-number and low-copy-number plsmid vectors for lacZ alpha-complementation and chloramphenicol-or kanamycin-resistance selection. Gene 61:63-74.
50. Tobe, T., G. K. Schoolnik, I. Sohel, V. H. Bustamante, and J. L. Puente. 1996. Cloning and characterization of bfpTVW, genes required for the transcriptional activation of bfpA in enteropathogenic Escherichia coli. Mol. Microbiol. 21:963-975.
51. Ussery, D. W., J. C. D. Hinton, B. J. A. M. Jordi, P. E. Granum, A. Seirafi, R. J. Stephen, A. E. Tupper, G. Berridge, J. M. Sidebotham, and C. F. Higgins. 1994. The chromatin-associated protein H-NS. Biochimie 76:968-980.
52. Vind, J., M. A. Sorensen, M. D. Rasmussen, and S. Pedersen. 1993. Synthesis of proteins in Escherichia coli is limited by the concentration of free ribosomes. J. Mol. Biol. 231:678-688.
53. Willshaw, G., M. M. McConnell, W. Gaastra, A. Thomas, M. Hibberd, and B. Row. 1990. Structural and regulatory genes for coli surface antigen 4 (CS4) are encoded by separate plasmids in enterotoxigenic E. coli strains of serotype O25.H42. FEMS Microbiol. Lett. 68:255-260.
54. Willshaw, G. A., H. R. Smith, M. M. McConnell, and B. Rowe. 1991. Cloning of regulator genes controlling fimbrial production by enterotoxigenic Escherichia coli. FEMS Microbiol. Lett. 82:125-130.