Serum PCR of Pneumocystis carinii DNA in immunocompromised patients

Scandinavian Journal of Infectious Diseases

Volumn 29, Issue 2, 1997, Pages 159-164

  Wagner, Dirk ^a,c   Königer, Jürgen ^b   Kern, Winfried V ^b   Kern, Peter ^b  

^a UNIVERSITY HOSPITAL TÜBINGEN   (Germany)

^b UNIVERSITY HOSPITAL ULM   (Germany)

^c UNIVERSITY OF TÜBINGEN   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

MITOCHONDRIAL RNA; PROTOZOAL DNA; RIBOSOME RNA;

ADULT; AGED; ARTICLE; BRONCHOSCOPY; CLINICAL ARTICLE; CLINICAL TRIAL; DIAGNOSTIC ACCURACY; DIAGNOSTIC VALUE; DNA DETERMINATION; FEMALE; HUMAN; HUMAN IMMUNODEFICIENCY VIRUS INFECTION; IMMUNE DEFICIENCY; LUNG LAVAGE; MALE; PNEUMOCYSTIS CARINII; PNEUMOCYSTIS PNEUMONIA; PNEUMOCYSTOSIS; POLYMERASE CHAIN REACTION; PROPHYLAXIS;

EID: 0030983130     PISSN: 00365548     EISSN: None     Source Type: Journal    
DOI: 10.3109/00365549709035877     Document Type: Article

References (23)

1. Smith DE, Forbes A, Davies S, Barton SE, Gazzard BG. Diagnosis of Pneumocystis carinii pneumonia in HIV antibody positive patients by simple outpatient assessments. Thorax 47: 1005-1009, 1992.
2. Smith JW, Bartlett MS. Laboratory diagnosis of pneumocystosis. Clin Lab Med 11: 957-975, 1991.
3. Wakefield AE, Pixley FP, Banerji S, Sinclair K, Miller RF, Moxon ER, Hopkin JM. Detection of Pneumocystis carinii with DNA amplification. Lancet 336: 451-453, 1990.
4. Kitada K, Oka S, Kimura S, Shimada K, Serikawa T, Yamada J, Tsunoo H, Egawa K, Nakamura Y. Detection of Pneumocystis carinii sequences by polymerase chain reaction: animal models an1d clinical application to noninvasive specimens. J Clin Microbiol 29: 1985-1990, 1991.
5. Schluger N, Godwin T, Sepkowitz K, Armstrong D, Bernard E, Rifkin M, Cerami A, Bucala R. Application of DNA amplification to pneumocystosis: presence of serum Pneumocystis carinii DNA during human and experimentally induced Pneumocystis carinii pneumonia. J Exp Med 176: 1327-1333, 1992.
6. Becker-Hapak M, Liberator P, Graves D. Detection of human Pneumocystis carinii by polymerase chain reaction. J Protozool 38: S191-S194, 1991.
7. Olsson M, Elvin K, Löfdahl S, Linder E. Detection of Pneumocystis carinii DNA in sputum and bronchoalveolar lavage samples by polymerase chain reaction. J Clin Microbiol 31: 221-226, 1993.
8. Wakefield AE, Guiver L, Miller RF, Hopkin JM. DNA amplification on induced sputum samples for diagnosis of Pneumocystis carinii pneumonia. Lancet 337: 1378-1379, 1991.
9. Leigh TR, Wakefield AE, Peters SE, Hopkin JM, Collins JV. Comparison of DNA amplification and immunofluorescence for detecting Pneumocystis carinii in patients receiving immunosuppressive therapy. Transplantations 54: 468-470, 1992.
10. Roux P, Lavard I, Poirot JL, Chouaid C, Denis M, Olivier JL, Nigou M, Miltgen M. Usefulness of PCR for detection of Pneumocystis carinii DNA. J Clin Microbiol 32: 2324-2326, 1994.
11. Chouaid C, Roux P, Lavard I, Poirot JL, Housset B. Use of polymerase chain reaction technique on induced-sputum samples for the diagnosis of Pneumocystis carinii pneumonia in HIV-infected patients. Am J Clin Pathol 104: 72-75, 1995.
12. Leibovitz E, Pollack H, Moore T, Papellas J, Gallo L, Krasinski K, Borkowsky W. Comparison of PCR and standard cytological staining for detection of Pneumocystis carinii from respiratory specimens from patients with or at high risk for infection by human immunodeficiency virus. J Clin Microbiol 33: 3004-3007, 1995.
13. Schluger N, Sepkowitz K, Armstrong D, Bernard E, Rifkin M, Cerami A, Bucala R. Detection of Pneumocystis carinii in serum of AIDS patients with Pneumocystis carinii pneumonia by polymerase chain reaction. J Protozool 38: S240-S242, 1991.
14. Lipschik GY, Gill VJ, Lundgren JD, Andrawis VA, Nelson NA, Nielsen JO, Ognibene FP, Kovacs JA. Improved diagnosis of Pneumocystis carinii infection by polymerase chain reaction on induced sputum and blood. Lancet 340: 203-205, 1992.
15. Tamburrini E, Mencarini P, de Luca A, Visconti E, Antinori A, Ortona E, Siracusano A. Detection of Pneumocystis DNA in serum and circulating cells is not a good diagnostic marker for pneumocystosis in HIV patients. J Euk Microbiol 41: S112, 1994.
16. Sepkowitz K, Schluger N, Godwin T, Armstrong D, Cerami A, Bucala R. DNA amplification in experimental pneumocystosis: characterization of serum Pneumocystis carinii DNA and potential P. carinii carrier states. J Infect Dis 168: 421-426, 1993.
17. Atzori C, Lu J-J, Bingdong B, Bartlett MS, Orlando G, Queener SF, Smith JW, Cargnel A, Lee C-H. Diagnosis of Pneumocystis carinii pneumonia in AIDS patients by using polymerase chain reaction on serum specimens. J Infect Dis 172: 1623-1626, 1995.
18. De Luca A, Ortona E, Margutti P, Visconti E, Tamburrini E, Siracusano E. Different amplification efficiency and nucleotide sequence variation in various Pneumocystis isolates from humans and rats. J Euk Microbiol 41: S85, 1994.
19. Saiki RK, Gelfand DH, Stoffel S, Scharf SJ, Higuchi R, Horn GT, Mullis KB, Erlich HA. Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 239: 487-491, 1988.
20. Sinclair K, Wakefield AE, Banerji S, Hopkin JM. Pneumocystis carinii organisms derived from rat and human hosts are genetically distinct. Mol Biochem Parasitol 45: 183-184, 1991.
21. Lu J-J, Chen CH, Bartlett MS, Smith JW, Lee CH. Comparison of six different PCR methods for the detection of Pneumocystis carinii. J Clin Microbiol, 1996 (in press).
22. Peters SE, Wakefield AE, Banerji S, Hopkin JM. Quantification of the detection of Pneumocystis carinii by DNA amplification. Mol Cell Probes 6: 115-117, 1992.
23. Tamburrini E, Mencarini P, Visconti E, Zolfo M, de Luca A, Siracusano A, Ortona E, Wakefield AE. Detection of Pneumocystis carinii DNA in blood by PCR is not of value for diagnosis of P. carinii pneumonia. J Clin Microbiol 34: 1586-1588, 1996.