Rapid purification of glial cells using immunomagnetic separation

Journal of Neuroscience Methods

Volumn 74, Issue 1, 1997, Pages 37-44

  Wright, A P ^a   Fitzgerald, J J ^a   Colello, R J ^a  

^a UNIVERSITY OF OXFORD   (United Kingdom)

Author keywords

Astrocyte; Cell culture; Dynabeads; O2 A progenitor cell

Indexed keywords

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; ASTROCYTE; CELL CULTURE; CELL ISOLATION; CONTROLLED STUDY; IMMUNOCYTOCHEMISTRY; NEWBORN; NONHUMAN; OPTIC NERVE; PRIORITY JOURNAL; RAT; STEM CELL;

ANIMALS; ASTROCYTES; CEREBRAL CORTEX; GLIAL FIBRILLARY ACIDIC PROTEIN; IMMUNOHISTOCHEMISTRY; IMMUNOMAGNETIC SEPARATION; NEUROGLIA; OPTIC NERVE; RATS; RATS, INBRED LEW; STEM CELLS;

EID: 0030982738     PISSN: 01650270     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0165-0270(97)22400-3     Document Type: Article

References (34)

1. Abney, E.R., Williams, B.P. and Raff, M.C. (1983) Tracing the development of oligodendrocytes from precursor cells using monoclonal antibodies, fluorescence-activated cell sorting and cell culture, Dev. Biol., 100: 166-171.
2. Barres, B.A., Chun, L.L.Y. and Corey, D.P. (1988) Ion channel in vertebrate glia, Annu. Rev. Neurosci., 13: 441-474.
3. Barres, B.A., Hart, I.K., Coles, H.S.R., Burne, J.F., Voyvodic, J.T., Richardson, W.D. and Raff, M.C (1992) Cell death and control of cell survival in the oligodendrocyte lineage, Cell, 70: 31-46.
4. Barres, B.A., Schmid, R., Sendnter, M. and Raff, M.C. (1993) Multiple extracellular signals are required for long-term oligodendrocyte survival, Development, 118: 283-295.
5. Bartlett, P.F., Noble, M.D., Pruss, R.M., Raff, M.C., Rattray, S. and Williams, C.A. (1981) Rat Neural Antigen-2 (RAN-2); a cell surface antigen on astrocytes, ependymal cells, muller cells and leptomeninges defined by a monoclonal antibody, Brain Res., 204: 339-351.
6. Chalazonitis, A., Rothman, T.P., Chen, J., Lambelle, F., Barbacid, M. and Gershon, M.D. (1994) Neurotrophin-3 induces neural crest-derived cells from fetal rat gut to develop in vitro as neurons or glia, J. Neurosci., 14: 6571-6584.
7. Colello, R.J., Wright, A.P. and Fitzgerald, J.J. (1995) Purification of glial cells using immunomagnetic separation, Soc. Neurosci. Abstr., 21: 321.
8. Collin-Osdoby, P., Ourlser, M.J., Webber, D. and Osdoby, P. (1991) Osteoclast-specific monoclonal antibodies coupled to magnetic beads provide a rapid and efficient method of purifying avian osteoclasts, J. Bone Miner. Res., 6: 1353-1365.
9. Egeland, T., Hovdenes, A. and Lea, T. (1988) Positive selective of antigen-specific B lymphocytes by means of immunomagnetic particals, Scand. J. Immunol., 27: 439-444.
10. Eisenbarth, G.S., Walsh, F.S., and Nirenburg, M. (1979) Monoclonal antibody to a plasma membrane antigen of neurons, Proc. Natl. Acad. Sci. USA, 76: 4913-4917.
11. Fedoroff, S., Neal, J., Opas, M. and Kalnins, V. (1984) Astrocyte cell lineage. III The morphology of differentiating mouse astrocytes in colony culture. J. Neurocytol., 13: 1-20.
12. Fei, R-G., Penn, P.E. and Wolf, N.S. (1990) A method to establish pure fibroblast and endothelial cell colony cultures from murine bone marrow, Exper. Hematol., 18: 953-957.
13. Fok-Seang, J., Mathews, G.A., French-Constant, C., Trotter, J. and Fawcett, J.W. (1995) Migration of oligodendrocyte precursors on astrocytes and meningeal cells, Dev. Biol., 171: 1-15.
14. Galileo, D.S., Gee, A.P. and Linser, P.J. (1991) Neurons are replensihed in cultures of embryonic chick optic tectum after immunomagnetic depletion, Dev. Biol., 146: 278-291.
15. Galileo, D.S. and Linser, P.J. (1992) Immunomagnetic removal of neurons from developing chick optic tectum results in glial phenotypic instability, Glia, 5: 210-222.
16. Horton, J.K., Evans, O.M., Swann, K. and Swinburn, S. (1989) A new rapid method for the selection and cloning of antigen-specific hybridomas with magnetic microspheres, J. Immunol. Methods., 124: 225-230.
17. Jackson, C.J., Garbett, P.K., Nissen, B. and Schrieber, L. (1990) Binding of human endothelium to Ulex europaeus 1-coated Dynabeads: application to the isolation of microvascular endothelium, J. Cell Sci., 96: 257-262.
18. Landis, S.C. (1976) Rat sympathetic neurons and cardiac myocytes developing in microcultures: correlation of the fine structure of endings with neurotransmitter function in single neurons, Proc. Natl. Acad. Sci., USA, 73: 4220-4224.
19. Lea, T., Smeland, E., Funderud, S., Vartdal, F., Davies, C., Beiske, D. and Ugelstad, J. (1986) Characterisation of human mononuclear cells after positive selection with immunomagnetic particles, Scand. J. Immunol., 23: 509-519.
20. Levison, S.W. and McCarthy, K.D. (1992) Astroglia in Culture. In Banker, G. and Goslin, K. (Ed.), Culturing Nerve Cells, The MIT Press, Cambridge, Mass., pp. 309-336.
21. Mage, M.G., McHugh, L.L. and Rothstein, T.L. (1977) Mouse lymphocytes with and without surface immunoglobulin: preparative scale separation on polystyrene tissue culture dishes coated with specifically purified anti-immunoglobulin, J. Immunol. Methods, 15: 47-56.
22. Manyonda, I.T., Solty, A.J. Hay, F.C. (1992) A critical evaluation of the magnetic cell sorter and its use in the positive and negative selection of CD45RO+ cells, J. Immunol. Methods, 149: 1-10.
23. McCarthy, K.D. and De Vellis, J. (1980) Preparation of separate astroglial and oligodendroglial cell cultures from rat cerebral tissue, J. Cell Biol., 85: 890-902.
24. Murphy, S.J., Watt, D.J. and Jones, G.E. (1992) An evaluation of cell separation techniques in a model mixed cell population, J. Cell Sci., 102: 789-798.
25. Patterson, P.H. and Chun, L.L.Y. (1977) The induction of acetylcholine synthesis in primary cultures of dissociated rat sympathetic neurons, Dev. Biol., 56: 263-280.
26. Pomeranz, H.D., Rothman, T.P., Chalazonitis, A., Tennyson, V.M., and Gershon, M.D. (1993) Neural crest-derived cells isolated from the gut by immunoselection develop neuronal and glial phenotypes when cultured on laminin, Dev. Biol., 156: 341-361.
27. Raff, M.C., Abney, E.A. and Miller, R.H. (1984) Two glial lineages diverge prenatally in rat optic nerve, Dev. Biol., 106: 53-60.
28. Rasmussen, A.M., Smeland, E., Erikstein, B.K., Caignault, L. and Funderud, S. (1992) A new method for detachment of Dynabeads from positively selected B lymphocytes. J. Immunol. Methods, 146: 185-202.
29. Richardson, W.D., Pringle, N., Mosley, M.J., Westermark, B. and Dubois-Dalcq, M. (1988) A role for platelet-derived growth factor in normal gliogenesis in the central nervous system, Cell, 53: 309-319.
30. Sasaki, A., Levison, S.W. and Ting, J.P-Y. (1989) Comparison and quantitation of Ia antigen expression on cultured macroglia and amoeboid microglia from Lewis rat cerebral cortex: analyses and implications, J. Neuroimmunol., 25: 63-74.
31. Shaw, G., Osborn, M. and Weber, K. (1981) Arrangement of neurofilaments, microtubules and microfilament-associated proteins in cultured dorsal root ganglia cells, Eur. J. Cell. Biol., 24: 20-27.
32. Tuttle, R. and Matthew, W.D. (1991) An in vitro bioassay for neurite growth using cryostat sections of nervous tissue as a substratum, J. Neurosci. Methods, 39: 193-202.
33. Wysocki, L.J. and Sato, V.L. (1978). 'Panning' for lymphocytes: a method for cell selection, Proc. Natl. Acad. Sci., USA 61: 477-483.
34. Zeller, N.K., Behar, T.N., Dubois-Dalcq, M.E. and Lazzarini, R.A. (1985) The timely expression of mylein basic protein gene in culturing rat brain oligodendrocytes in independent of continuous neuronal influences, J. Neurosci., 5: 2955-2962.