Characterization of Three Human Apolipoprotein E Isoforms (E2, E3 and E4) Expressed in Escherichia coli

Clinical Chemistry and Laboratory Medicine

Volumn 35, Issue 8, 1997, Pages 581-590

  Barbier, Anne ^a   Visvikis, Athanase ^a   Mathieu, Florence ^a   Diez, Laurent ^a   Siest, Gérard ^a   Havekes, Louis M ^b  

^a Centre du Medicament URA CNRS 597   (France)

^b TNO   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

APOLIPOPROTEIN E2; APOLIPOPROTEIN E3; APOLIPOPROTEIN E4; MONOCLONAL ANTIBODY; VERY LOW DENSITY LIPOPROTEIN;

ARTICLE; CHOLESTEROL METABOLISM; ESCHERICHIA COLI; NONHUMAN; PRIORITY JOURNAL; RECEPTOR BINDING;

ANIMALS; ANTIBODIES, MONOCLONAL; APOLIPOPROTEIN E2; APOLIPOPROTEIN E3; APOLIPOPROTEIN E4; APOLIPOPROTEINS E; BIOSENSING TECHNIQUES; CELL LINE; ESCHERICHIA COLI; GENETIC VECTORS; HUMANS; ISOMERISM; MACROPHAGES; MICE; MICE, KNOCKOUT; RECEPTORS, LDL; RECOMBINANT PROTEINS;

ESCHERICHIA COLI;

EID: 0030982566     PISSN: 14346621     EISSN: 14374331     Source Type: Journal    
DOI: 10.1515/cclm.1997.35.8.581     Document Type: Article

References (31)

1. Mahley RW, Innerarity TL, Rail, Jr. SC, Weisgraber KH, Plasma lipoproteins: apolipoprotein structure and function. J Lipid Res 1984; 25: 1277-1294.
2. Zannis VI, Breslow J. Synthesis, intracellular processing and signal peptide of human apolipoprotein E. J Biol Chem 1984; 259: 5495-5499.
3. Mahley RW, Apolipoprotein E: cholesterol transport protein with expanding role in cell biology. Science 1988; 240: 622-630.
4. Pitas RE, Boyles JK, Lee SH, Foss D, Mahley RW, Astrocytes synthesize apolipoprotein E and metabolize apolipoprotein E-containing lipoproteins. Biochim Biophys Acta 1987; 917: 148-161.
5. Zannis VI, Just PW, Breslow JL, Human apolipoprotein E isoprotein subclasses are genetically determined. Am J Hum Genet 1981; 33: 11-24.
6. Utermann G, Steinmetz A, Weber W. Genetic control of human apolipoprotein E polymorphism: comparison of one- and two dimensional techniques of isoprotein analysis. Hum Genet 1982; 60: 344-351.
7. Weisgraber KH, Roses AD, Strittmatter WJ, The role of apolipoprotein E in the nervous system. Curr Opin in Lipidol 1994; 5: 110-116.
8. Takahashi S, Kawarabayasi Y, Nakai T, Sakai J, Yamamoto T. Rabbit very low density lipoprotein receptor: a low density lipoprotein receptor-like protein with distinct ligand specificity. Proc Natl Acad Sci USA 1992; 89: 9252-9256.
9. Bihain BE, Delplanque B, Khallou J, Chevreuil O, Troussard AA, Michel L, et al. Lipolysis-stimulated receptor: a newcomer on the lipoprotein research scene. Diabetes & Metab 1995; 21: 121-126.
10. Kim DH, Iijima H, Goto K, Sakai J, Ishii H, Kim HJ, et al. Human apolipoprotein E receptor 2. J Biol Chem 1996; 271: 8373-8380.
11. Lalazar A, Weisgraber KH, Rail Jr SC, Giladi H, Innerarity TL, Levanon AZ, et al. Site-specific mutagenesis of human apolipoprotein E: receptor binding activity of variants with single amino acid substitutions. J Biol Chem 1988; 263: 3542-3545.
12. Dong LM, Parkin S, Trakhanov SD, Rupp B, Simmons T, Arnold KS, et al. Novel mechanism for defective receptor binding of apolipoprotein E in type III hyperlipoproteinemia. Nature Struct Biol 1996; 3: 718-722.
13. Davignon J, Gregg RE, Sing CF, Apolipoprotein E polymorphism and atherosclerosis. Arteriosclerosis 1988; 8: 1-21.
14. van Bockxmeer FM, Apolipoprotein E and Alzheimer's. Nature 1995; 375: 285.
15. Corder EH, Saunders AM, Strittmatter WJ, Schmechel DE, Gaskell PC, Small GW, et al. Gene dose of apolipoprotein E type 4 allele and the risk of Alzheimer's disease in late onset families. Science 1993; 261: 921-923.
16. Poirier J. Apolipoprotein E in animal models of brain injury and in Alzheimer's disease. Trends Neurosci 1994; 12: 525-530.
17. Ohm TG, Kirca M, Bohl J, Schnamagl H, Groß W, März W. Apolipoprotein E polymorphism influences not only cerebral senile plaque load but also Alzheimer-type neurofibrillary tangle formation. Neuroscience 1995; 66: 583-587.
18. Schächter F, Faure-Delanef L, Guenot F, Rouger H, Frogel P, Lesueur-Ginot L, et al. Genetics associations with human longevity at the APOE and ACE loci. Nature Genet 1994; 6: 29-32.
19. Corder EH, Saunders AM, Risch NJ, Strittmatter WJ, Schmechel DE, Gaskell PC, et al. Protective effect of apolipoprotein E type 2 allele for late onset Alzheimer's disease. Nature Genet 1994; 7: 180-184.
20. Pillot T, Barbier A, Lozac'h K, Visvikis A, Rosseneu M, Vand-erkerckhove J, et al. Single step purification of two functional apolipoprotein E variants hyperexpressed in Escherichia coli. Expr Purif 1996; 7: 407-414.
21. Milne RW, Douste-Blazy PH, Marcel YL, Characterization of monoclonal antibodies against human apolipoprotein E. J Clin Invest 1981; 68: 111-117.
22. Studier FW, Moffatt BA, Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes. J Mol Biol 1986; 189: 13-30.
23. Dergunov AD, Aniskovich LP, Shuvaev VV, Affinity chromatography on heparin-sepharose under reducing conditions as a method of selective enrichment with individual isoforms of apolipoprotein E. Bull Exper Biol Med 1990; 110: 42-45.
24. Chaiken I, Rosé S, Karlsson R. Analysis of macromolecular interactions using immobilized ligands. Anal Biochem 1992; 201: 197-210.
25. Karlsson R, Michaelsson A, Mattsson L. Kinetic analysis of monoclonal antibody-antigen interactions with a new biosensor based analytical system. J Immunol Meth 1991; 145: 299-310.
26. Gerdes, K. The parB(hokJsok:) locus of plasmid R1: a general purpose plasmid stabilization system. Biotechnology 1988; 6: 1402-1405.
27. Vogel T, Weisgraber KH, Zeevi ML, Ben-Artzi H, Levanon AZ, Rail, Jr. SC, et al. Human apolipoprotein E expression in Escherichia coli: structural and functional identity of the bacterially produced protein with plasma apolipoprotein E. Proc Natl Acad Sci USA 1985; 82: 8696-8700.
28. Monteilhet C, Lachacinski N, Aggerbeck LP, Cytoplasmic and periplasmic production of human apolipoprotein E in Escherichia coli using natural and bacterial signal peptides. Gene 1992; 125: 223-228.
29. Gretch DG, Sturley SL, Friesen PD, Beckage NE, Attie AD, Baculovirus-mediated expression of human apolipoprotein E in Manduca sexta larvae generates particles that bind to the low density lipoprotein receptor. Proc Natl Acad Sci USA 1991; 88: 8530-8533.
30. Riffai R, Maurice R, Weisgraber K, Innerarity T, Wang X, MacKenzie R, et al. Molecular characterization of two monoclonal antibodies specific for the LDL receptor-binding site of human apolipoprotein E. J Lipid Res 1995; 36: 1905 -1918.
31. Weisgraber KH, Innerarity TL, Mahley RW, Abnormal lipoprotein receptor-binding activity of the human E apoprotein due to cystein-arginine interchange at a single site. J Biol Chem 1982; 257: 2518-2521.