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note
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Yeast strains were constructed by transforming the strain YRG-2 (Stratagene) with the indicated open reading frames inserted into the vectors pGAD-GH and pAS2, which respectively contain Leu or Trp selectable markers. Strains were assayed for growth on synthetic dropout complete (SDC)-leu/trp/his plates containing 50 mM 3-aminotriazole. β-Galactosidase (β-Gal) assays were performed with ONPG. Each strain was assayed by protein immunoblot for expression of the appropriate fusion proteins.
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1842279800
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note
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2, 10% glycerol, and 25 mM Hepes (pH 7.0)] was substituted for bv150.
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40
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1842385559
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note
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2, and 10% glycerol (pH 7.4) was used in the combinations listed. Agarose gels (0.6%) were run in 1x tris-borate/EDTA or glycine buffer.
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note
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We thank C. Wu and H. Ueda for antibodies to FTZF1, Y. Kageyama and H. Ueda for sharing data before publication, and M. Fish for assistance in generating transgenic Drosophila strains. K.P.W. is a predoctoral fellow of the Howard Hughes Medical Institute, P.H. is a postdoctoral fellow of the American Cancer Society, and T.W. was supported by a postdoctoral fellowship from the Burroughs Wellcome Fund of the Life Science Research Foundation. This work was also supported by a grant from NIH to D.S.H.
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