PCR mutagenesis: Treatment of the megaprimer with mung bean nuclease improves yield

BioTechniques

Volumn 22, Issue 6, 1997, Pages 1054-1056

  Chattopadhyay, Debasish ^a   Raha, Tamal ^a   Chattopadhyay, Dhrubajyoti ^a  

^a UNIVERSITY OF CALCUTTA   (India)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; DNA HYBRIDIZATION; ENZYME ACTIVITY; ENZYME ANALYSIS; GENE AMPLIFICATION; OLIGONUCLEOTIDE PROBE; POLYMERASE CHAIN REACTION; SITE DIRECTED MUTAGENESIS;

PHASEOLUS (ANGIOSPERM); VIGNA RADIATA VAR. RADIATA;

EID: 0030981232     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/97226bm09     Document Type: Article

References (9)

1. Barik, S. 1993. Site-directed mutagenesis by double polymerase chain reaction: megaprimer method. Methods Mol. Biol. 15:277-286.
2. Barik, S. and M.S. Galinski. 1991. "Megaprimer" method of PCR: increased template concentration improves yield. BioTechniques 10:489-490.
3. Clark, J.M. 1988. Novel nontemplated nucleotide addition reaction catalysed by prokaryotic and eukaryotic DNA polymerases. Nucleic Acids Res. 16:9677-9686.
4. Kroeker, W.D. and D. Kowalski. 1978. Gene-sized pieces produced by digestion of linear duplex DNA with mung bean nuclease. Biochemistry 17:3236-323.
5. Kuipers, O.P., H.J. Boot and W.M. Vos. 1991. Improved site-directed mutagenesis. Nucleic Acids Res. 19:4558.
6. Landt, O., H.P. Grunert and U. Hahn. 1990. A general method for rapid site-directed mutagenesis using polymerase chain reaction. Gene 96:125-128.
7. Picard, V., E. Badju, A. Lu and S.C. Bock. 1994. A rapid and efficient one-tube PCR-based mutagenesis technique using Pfu DNA polymerase. Nucleic Acids Res. 22:2587-2591.
8. SamBrook, J., E.F. Fritsch and T. Maniatis. 1989. Molecular Cloning: A Laboratory Manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
9. Sarkar, G. and S.S. Sommer. 1990. The "megaprimer" method of site-directed mutagenesis. BioTechniques 8:404-407.