Spatial distribution of dihydropyridine receptors in the plasma membrane of guinea pig cardiac myocytes investigated by correlative confocal microscopy and label-fracture electron microscopy

Journal of Electron Microscopy

Volumn 46, Issue 2, 1997, Pages 165-170

  Takagishi, Yoshiko ^a,c   Rothery, Stephen ^a   Issberner, Jon ^b   Levi, Allan ^b   Severs, Nicholas J ^a  

^a ROYAL BROMPTON HOSPITAL   (United Kingdom)

^b UNIVERSITY OF BRISTOL   (United Kingdom)

^c NAGOYA UNIVERSITY   (Japan)

Author keywords

Cardiac muscle cell; Confocal microscopy; Dihydropyridine receptors; Freeze fracture cytochemistry; L type calcium channels

Indexed keywords

ANIMALIA; CAVIA PORCELLUS; SUS SCROFA;

CALCIUM; CELL MEMBRANES; FRACTURE; MAMMALS; MUSCLE;

CALCIUM RELEASE; CARDIAC MUSCLE CELL; CARDIAC MUSCLES; DIHYDROPYRIDINE RECEPTORS; FREEZE FRACTURE; FREEZE-FRACTURE CYTOCHEMISTRY; GUINEA PIGS; L-TYPE CALCIUM CHANNELS; MUSCLE CELL; SARCOPLASMIC RETICULUM;

CONFOCAL MICROSCOPY;

EID: 0030980753     PISSN: 00220744     EISSN: None     Source Type: Journal    
DOI: 10.1093/oxfordjournals.jmicro.a023504     Document Type: Article

References (18)

1. Fabiato A (1983) Calcium-induced release of calcium from the cardiac sarcoplasmic reticulum. Am. J. Physiol. 245 : C1-C14.
2. Valdeolmillos M, O'Neill S C, Smith G L, and Eisner D A (1989) Calcium-induced calcium release activates contraction in intact cardiac cells. Pflugers Arch. 413: 676-678.
3. i waves in cardiac myocytes. Am. J. Physiol. Cell. Physiol. 270: C148-C159.
4. Cannell M B, Cheng H, and Lederer W J (1995) The control of calcium release in heart muscle. Science 268: 1045-1049.
5. Cari S L, Felix K, Caswell A H, Brandt N R, Ball W J, Jr, Vaghy P L, Meissner G, and Ferguson D G (1995) Immunolocalization of sarcolemmal dihydropyridine receptor and sarcoplasmic reticular triadin and ryanodine receptor in rabbit ventricle and atrium. J. Cell Biol. 129: 673-682.
6. Sun X-H, Protasi F, Takahashi M, Takeshima H, Ferguson D G, and Franzini-Armstrong C (1995) Molecular architecture of membranes involved in excitation-contraction coupling of cardiac muscle. J. Cell Biol. 129: 659-671.
7. Protasi F, Sun X-H, and Franzini-Armstrong C (1996) Formation and maturation of the calcium release apparatus in developing and adult avian myocardium. Dev. Biol. 173: 265-278.
8. 2+ sparks and local control theories for cardiac excitation-contraction coupling. Circ. Res. 78: 166-171.
9. 2+ exchanger. FEBS Lett. 355: 65-68.
10. Pinto da Silva P and Kan F W K (1984) Label-fracture: a method for high resolution labeling of cell surfaces. J. Cell Biol. 99: 1156-1161.
11. Kan F W K and Pinto da Silva P (1989) Label-fracture cytochemistry. In Hayat M A, ed., Colloidal Gold. Principles, Methods and Applications, pp.175-201, (Academic Press, Orlando London).
12. Severs N J (1995) Freeze-fracture cytochemistry: an explanatory survey of methods. In Severs N J and Shotton D M, eds, Rapid Freezing, Freeze Fracture, and Deep Etching, pp. 173-208, (Wiley-Liss Inc., New York).
13. Takagishi Y, Issberner J, Rothery S, Levi A J, and Sever N J (1996) Dihydropyridine receptors (calcium channels) are organized in clusters in the plasma membrane of isolated guinea-pig cardiac myocytes. J. Physiol. 491: 163P (Abstract).
14. Powell T, Terrar D A, and Twis, V W (1980) Electrical properties of individual cells isolated from rat ventricular myocardium. J. Physiol. (Lond.) 302: 131-153.
15. Mitchell M R, Powell T, Terrar D A, and Twist V W (1987) Electrical activity and contraction in cells isolated from rat and guinea pig ventricular muscle: a comparative study. J. Physiol. (Lond.) 391: 527-544.
16. Ahlijanian M K, Westenbroek R E, and Catterall W A (1990) Subunit structure and localization of dihydropyridine-sensitive calcium channels in mammalian brain, spinal cord, and retina. Neuron 4: 819-832.
17. Pauli B U, Weinstein R S, Soble L W, and Alroy J (1977) Freeze-fracture of monolayer cultures. J. Cell Biol. 72: 763-769.
18. Brown D (1981) Polyvinyl alcohol coating: an improvement of the freeze-fracture technique. J. Microsc. 121: 283-287.