Regulation of trypsin activity by Cu2+ chelation of the substrate binding site

Protein Engineering

Volumn 10, Issue 5, 1997, Pages 551-560

  Briand, L ^a   Chobert, J M ^a   Tauzin, J ^a   Declerck, N ^b   Leonil J ^c   Molle D ^c   Tran, V ^d   Haertle T ^a  

^a CEMAGREF   (France)

^b AGROPARISTECH   (France)

^c LRTL   (France)

^d LPCM   (France)

Author keywords

Inhibition; Metal chelation; Serine protease; Site directed mutagenesis; Trypsin; casein

Indexed keywords

ARGININE; BETA CASEIN; COPPER ION; EDETIC ACID; HISTIDINE; HYDROLASE; LYSINE; MUTANT PROTEIN; PROTEINASE; TETRAPEPTIDE; TRYPSIN;

AMINO ACID SEQUENCE; ARTICLE; BINDING SITE; CHELATION; COMPUTER MODEL; CONTROLLED STUDY; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME BINDING; ENZYME INHIBITION; ENZYME KINETICS; ENZYME REGULATION; ENZYME SUBSTRATE; ENZYME SUBSTRATE COMPLEX; GENE MUTATION; METAL BINDING; MOLECULAR MODEL; NONHUMAN; PH; PRIORITY JOURNAL; SITE DIRECTED MUTAGENESIS;

FELIS CATUS;

EID: 0030979726     PISSN: 02692139     EISSN: None     Source Type: Journal    
DOI: 10.1093/protein/10.5.551     Document Type: Article

References (27)

1. Briand, L., Chobert, J.M. and Haertlé, T. (1994) Milchwissenschaft, 49, 367-371.
2. Brinen, L.S., Willett, W.S., Craik, C.S. and Fletterick, R.J. In Protein Data Bank, 11 July 1996.
3. Carles, C. and Ribadeau Dumas, B. (1986) J. Dairy Res., 53, 595-600.
4. Carter, P. and Wells, J.A. (1987) Science, 237, 394-399.
5. Corey, D.R. and Craik, C.S. (1992) J. Am. Chem. Soc., 114, 1784-1790.
6. Corey, D.R. and Schultz, P.G. (1989) J. Biol. Chem., 264, 3666-3669.
7. Corey, D.R., Willett, W.S., Coombs, G.S. and Craik, C.S. (1995) Biochemistry, 34, 11521-11527.
8. Craik, C.S., Choo, Q., Swift, G.H., Quint, C., McDonald, R.J. and Rutter, R.J. (1984) J. Biol. Chem., 259, 14255-14264.
9. Evnin, L.B., Vasquez, J.R. and Craik, C.S. (1990) Proc. Natl Acad. Sci. USA, 87, 6659-6663.
10. Fersht, A.R. (1985) In Freeman, W.H. et al. (ed.), Enzyme Structure and Mechanism, 2nd edn. New York, p. 475.
11. Graf, L., Craik, C.S., Pathy, A., Roczniak, S., Fletterick, R.J. and Rutter, W.J. (1987) Biochemistry, 26, 2616-2623.
12. Graf, L., Jansco, A., Szilagyi, L., Hegyi, G., Pinter, K., Naray-Szabo, G., Hepp, J., Medzihradsky, K. and Rutter, W.J. (1988) Proc. Natl Acad. Sci. USA, 85, 4961-4965.
13. Higaki, J.N., Haymore, B.L., Chen, S., Fletterick, R.J. and Craik, C.S. (1990) Biochemistry, 29, 8582-8586.
14. Jameson, G.W., Roberts, D.V., Adams, W.S., Kyle, S.A. and Elmore, D.T. (1973) Biochem. J., 131, 107-117.
15. Kunkel, T.A. (1985) Proc. Natl Acad. Sci. USA, 82, 488-192.
16. Laemmli, U.K. (1970) Nature, 225, 650-657.
17. Léonil, J., Mollé, D. and Maubois, J.L. (1988) Lait, 68, 281-294.
18. Martell, A.D. and Smith, R.M. (1974) In Critical Stability Constants, Plenum Press, NY
19. Matsumura, M. and Matthews, B.W. (1989) Science, 243, 792-794.
20. Mercier, J.C., Maubois, J.L., Poznanski, S. and Ribadeau Dumas, B. (1968) Bull. Soc. Chim. Biol., 50, 521-530.
21. Perona, J.J., Tsu, C.A., McGrath, M.E., Craik, C.S. and Fletterick, R.J. (1993) J. Mol. Biol., 230, 934-949.
22. Ribadeau Dumas, B., Brignon, G., Grosclaude, F. and Mercier, J.C. (1972) Eur. J. Biochem., 25, 505-514.
23. Sanger, F., Nicklen, S. and Coulson, A.R. (1977). Proc. Natl Acad. Sci. USA, 74, 5463-5467.
24. Vasquez, J.R., Evnin, L.B., Higaki, J.N. and Craik, C.S. (1989) J. Cell. Biochem., 39, 256-276.
25. Weiner, S.J., Kollman, P.A., Case, D.A., Singh, U.C., Ghio, C., Alagona, G., Profeta, S., Jr and Weiner, P. (1984) J. Am. Chem. Soc., 106, 765-784.
26. Willet, W.S., Gillmor, S.A., Perona, J.J., Fletterick, R.J. and Craik, C.S. (1995) Biochemistry, 34, 2172-2180.
27. Zittle, C.A. and Custer, J.H. (1963) J. Dairy Sci., 46, 1183-1188.