An indirect immunofluorescence assay using a cell culture-derived antigen for detection of antibodies to the agent of human granulocytic ehrlichiosis

Journal of Clinical Microbiology

Volumn 35, Issue 6, 1997, Pages 1510-1516

  Nicholson, William L ^a,b   Comer, James A ^a   Sumner, John W ^a   Gingrich Baker, Cindy ^c   Coughlin, Richard T ^c   Magnarelli, Louis A ^d   Olson, James G ^a   Childs, James E ^a  

^a CENTERS FOR DISEASE CONTROL AND PREVENTION   (United States)

^b JOHNS HOPKINS BLOOMBERG SCHOOL OF PUBLIC HEALTH   (United States)

^c Aquila Biopharmaceuticals Inc   (United States)

^d The Connecticut Agricultural Experiment Station   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBODY DETECTION; ANTIGEN EXPRESSION; ARTICLE; CELL STRAIN HL 60; CONTROLLED STUDY; EHRLICHIA; EHRLICHIOSIS; HUMAN; IMMUNOFLUORESCENCE TEST; PRIORITY JOURNAL;

ACARI; ANAPLASMA PHAGOCYTOPHILUM; ANAPLASMATACEAE; ANIMALIA; BOVINAE; CAPRA HIRCUS; EHRLICHIA; EQUUS CABALLUS;

EID: 0030978303     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/jcm.35.6.1510-1516.1997     Document Type: Article

References (27)

1. Anderson, B. E. 1994. PCR detection of Rickettsia rickettsii and Ehrlichia chaffeensis, p. 197-202. In D. H. Persing, T. F. Smith, F. C. Tenover, and T. J. White (ed.), Diagnostic molecular microbiology: principles and applications. American Society for Microbiology, Washington, D.C.
2. Bakken, J. S., J. S. Dumler, S. M. Chen, M. R. Eckman, L. L. Van Etta, and D. H. Walker. 1994. Human granulocytic ehrlichiosis in the upper midwest United States: a new species emerging? JAMA 272:212-218.
3. Beutner, E. H., S. A. Krasny, and T. P. Chorzelski. 1987. Defined immunofluorescence in immunodermatology, p. 3-40. In E. H. Beutner, T. P. Chorzelski, and V. Kumar (ed.), Immunopathology of the skin, 3rd ed. John Wiley and Sons, New York, N.Y.
4. Brouqui, P., J. S. Dumler, R. Leinhard, M. Brossard, and D. Raoult. 1995. Human granulocytic ehrlichiosis in Europe. Lancet 346:782-783.
5. Chen, S.-M., J. S. Dumler, J. S. Bakken, and D. H. Walker. 1994. Identification of a granulocytotropic Ehrlichia species as the etiologic agent of human disease. J. Clin. Microbiol. 32:589-595.
6. Collins, S. J., R. C. Gallo, and R. E. Gallagher. 1977. Continuous growth and differentiation of human myeloid leukaemic cells in suspension culture. Nature 270:347-349.
7. Corstvet, R. E., S. D. Gaunt, P. A. Karns, J. W. McBride, R. A. Battistini, L. A. Mauterer, and F. W. Austin. 1993. Detection of humoral antigen and antibody by enzyme-linked immunosorbent assay in horses with experimentally induced Ehrlichia equi infection. J. Vet. Diagn. Invest. 5:37-39.
8. Coughlin, R. T., C. Gingrich-Baker, C. Murphy, T. Mather, and D. Fish. 1995. Unpublished data.
9. Dumler, J. S., K. M. Asanovich, J. S. Bakken, P. Richter, R. Kimsey, and J. E. Madigan. 1995. Serologic cross-reactions among Ehrlichia equi, Ehrlichia phagocytophila, and the human granulocytic ehrlichia. J. Clin. Microbiol. 33:1098-1103.
10. Dumler, J. S., and J. S. Bakken. 1995. Ehrlichial diseases of humans: emerging tick-borne infections. Clin. Infect. Dis. 20:1102-1110.
11. Goodman, J. L., C. Nelson, B. Vitale, J. E. Madigan, J. S. Dumler, T. J. Kurtti, and U. G. Munderloh. 1996. Direct cultivation of the causative agent of human granulocytic ehrlichiosis. N. Engl. J. Med. 334:209-215.
12. Greig, B., K. M. Asanovich, P. J. Armstrong, and J. S. Dumler. 1996. Geographic, clinical, serologic, and molecular evidence of granulocytic ehrlichiosis, a likely zoonotic disease, in Minnesota and Wisconsin dogs. J. Clin. Microbiol. 34:44-48.
13. Hardeng, F. 1991. A modification of the indirect immunofluorescence test for detection of Ehrlichia phagocytophila antibodies. Acta Vet. Scand. 32: 499-502.
14. Johansson, K.-E., B. Pettersson, M. Uhlen, A. Gunnarsson, M. Malmqvist, and E. Olsson. 1995. Identification of the causative agent of granulocytic ehrlichiosis in Swedish dogs and horses by direct solid phase sequencing of PCR products from the 16S rRNA gene. Res. Vet. Sci. 58:109-112.
15. Jongejan, F., L. A. Wassink, M. J. C. Thielemans, N. M. Perie, and G. Uilenberg. 1989. Serotypes in Cowdria ruminantium and their relationship with Ehrlichia phagocytophila determined by immunofluorescence. Vet. Microbiol. 21:31-40.
16. Landis, J. R., and G. G. Koch. 1977. The measurement of observer agreement for categorical data. Biometrics 33:159-174.
17. Liz, J. S. 1994. Ehrlichia phagocytophila: aspects epidemiologiques, hematologiques et serologiques de l'infection chez les bovins en Suisse. Sc.D. thesis. University of Neuchatel, Neuchatel, Switzerland.
18. Madigan, J. E., J. E. Barlough, J. S. Dumler, N. S. Schankman, and E. DeRock. 1996. Equine granulocytic ehrlichiosis in Connecticut caused by an agent resembling the human granulocytotropic ehrlichia. J. Clin. Microbiol. 34:434-435.
19. Madigan, J. E., S. Hietala, S. Chalmers, and E. DeRock. 1990. Seroepidemiologic survey of antibodies to Ehrlichia equi in horses of northern California. J. Am. Vet. Med. Assoc. 196:1962-1964.
20. Magnarelli, L. A., J. S. Dumler, J. F. Anderson, R. C. Johnson, and E. Fikrig. 1995. Coexistence of antibodies to tick-borne pathogens of babesiosis, ehrlichiosis, and Lyme borreliosis in human sera. J. Clin. Microbiol. 33:3054-3057.
21. Nicholson, W. L., and J. A. Comer. 1996. Unpublished data.
22. Paxton, E. A., and G. R. Scott. 1989. Detection of antibodies to the agent of tick-borne fever by indirect immunofluorescence. Vet. Microbiol. 21:133-138.
23. Richter, P. J., Jr., R. B. Kimsey, J. E. Madigan, J. E. Barlough, J. S. Dumler, and D. L. Brooks. 1996. Ixodes pacificus (Acari: Ixodidae) as a vector of Ehrlichia equi (Rickettsiales: Ehrlichiae). J. Med. Entomol. 33:1-5.
24. Saah, A. J., and D. R. Hoover. 1997. "Sensitivity" and "specificity" reconsidered: the meaning of these terms in analytical and diagnostic settings. Ann. Intern. Med. 126:91-94.
25. Schenk, E. A., and C. J. Churukian. 1974. Immunofluorescence counterstains. J. Histochem. Cytochem. 22:962-966.
26. Sumption, K. J., D. J. M. Wright, S. J. Cutler, and B. A. S. Dale. 1995. Human granulocytic ehrlichiosis in the UK. Lancet 346:1487-1488.
27. Wilson, K. H. 1994. Characterization of novel bacterial pathogens by amplification and sequencing of 16S ribosomal RNA genes, p. 649-663. In G. D. Ehrlich and S. J. Greenberg (ed.), PCR-based diagnostics in infectious diseases. Blackwell Scientific Publications, Boston, Mass.