Rapid isolation of total RNA from small samples of hypocellular, dense connective tissues

BioTechniques

Volumn 22, Issue 6, 1997, Pages 1082-1086

  Reno, C ^a   Marchuk, L ^a   Sciore, P ^a   Frank, C B ^a   Hart, D A ^a  

^a UNIVERSITY OF CALGARY   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

PROTEOGLYCAN;

ARTICLE; CONNECTIVE TISSUE; GENE EXPRESSION; GENE ISOLATION; NORTHERN BLOTTING; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA ANALYSIS;

EID: 0030976489     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/97226bm16     Document Type: Article

References (12)

1. Adams, M.E., D.Q. Huang, L.Y. Yao and L.J. Sandell. 1992. Extraction and isolation of mRNA from adult articular cartilage. Anal. Biochem. 202:89-95.
2. Boykiw, R.H., C. Reno, P.J. Sciore and D.A. Hart. PCR cloning and sequencing of rabbit-specific cDNA for matrix molecules, proteinases, inhibitors and hormone receptors: detection of mRNA species in knee ligaments by RT-PCR; Abstract No. 40, p. 22. 3rd Pan Pacific Connective Tissue Symposium. 1996 November 3-December 6; Hawaii, HI.
3. Chomczynski, P. and N. Sacchi. 1987. Single step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem. 162:156-159.
4. Dourado, G.S. Level of mRNA expression for proteoglycans in osteoarthritis. Calgary, AB, Canada. University of Calgary; 1995. M. Sc. Thesis.
5. Frank, C.B., S.L.Y. Woo, T.P. Andriacchi, R.A. Brand, B. Oakes, L.E. Dahners, K. Dehaven, J. Lewis and C.P. Sabiston. 1988. Normal ligament: structure, function and composition, p. 45-101. In S.L.Y. Woo and J.A. Buckwalter (Eds.), Injury and Repair of the Musculoskeletal Soft Tissues. American Academy of Orthopaedic Surgeons. Park Ridge.
6. Kääpä, E., L.-Q. Zhang, P. Muona, S. Holm, H. Vanharanta and J. Peltonen. 1994. Expression of type I, III, and VI collagen mRNAs in experimentally injured porcine intervertebral disc. Connect. Tissue Res. 30:203-214.
7. Lenz, S.P. and D.A. Hart. 1997. LiCl treatment of female NZB/W mice leads to alterations in gene expression in renal tissue. J. Trace Microprobe Techn. 15:117-125.
8. Murphy, P.G. and D.A. Hart. 1993. Plasminogen activators and plasminogen activator inhibitors in connective tissues and connective tissue cells: influence of the neuropeptide substance P on expression. Biochim. Biophys. Acta 1182:205-214.
9. Re, P., W.B. Valhmu, M. Vostrejs, D.S. Howell, S.G. Fischer and A. Ratcliffe. 1995. Quantitative polymerase chain reaction assay for aggrecan and link protein gene expression in cartilage. Anal. Biochem. 225:356-360.
10. Sambrook, J., E.F. Fritsch and T. Maniatis. 1989. Molecular Cloning: A Laboratory Manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
11. Simms, D., P.E. Cizdziel and P. Chomczynski. 1993. TRIZOL™: a new reagent for optimal single-step isolation of RNA. Focus 15:99-103.
12. Watson, A.J., A. Hogan, A. Hahnel, K.E. Wiemer and G.A. Schultz. 1992. Expression of growth factor ligand and receptor genes in the preimplantable bovine embryo. Mol. Rep. Dev. 31:87-95.