Effects of gelatin and BSA on the amplification reaction for generating RAPD

BioTechniques

Volumn 22, Issue 6, 1997, Pages 1064-1066

  Stommel, John R ^a,c   Panta, Ganesh R ^a   Levi, Amnon ^b   Rowland, Lisa J ^a  

^a AGRICULTURAL RESEARCH SERVICE   (United States)

^b UNIVERSITY OF GEORGIA   (United States)

^c AGRICULTURAL RESEARCH SERVICE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DNA FRAGMENT; DNA POLYMERASE; GELATIN;

ARTICLE; CLOSTRIDIUM PERFRINGENS; DNA DETERMINATION; ENZYME ACTIVITY; GENE AMPLIFICATION; POLYMERASE CHAIN REACTION; RANDOM AMPLIFIED POLYMORPHIC DNA; REPRODUCIBILITY;

CLOSTRIDIUM PERFRINGENS;

EID: 0030976488     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/97226bm11     Document Type: Article

References (10)

1. Barry, T., G. Colleran, M. Glennon, L.K. Dunican and F. Gannon. 1991. The 16s/23s ribosomal spacer region as a target for DNA probes to identify eubacteria. PCR Methods Appl. 1:51-56.
2. Foolad, M.R., R.A. Jones and R.L. Rodriguez. 1993. RAPD markers for constructing intraspecific tomato genetic maps. Plant Cell Rep. 12:293-297.
3. Innis, M.A., K.B. Myambo, D.H. Gelfand and M.A.D. Brow. 1988. DNA sequencing with Thermus aquaticus DNA polymerase and direct sequencing of polymerase chain reaction-amplified DNA. Proc. Natl. Acad. Sci. USA 85:9436-9440.
4. Levi, A., L.J. Rowland and J.S. Hartung. 1993. Production of reliable randomly amplified polymorphic DNA (RAPD) markers from DNA of woody plants. HortScience 28:1188-1190.
5. Murray, M.G. and W.F. Thompson. 1980. Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res. 8:4321-4325.
6. Rowland, L.J. and B. Nguyen. 1993. Use of polyethylene glycol for purification of DNA from leaf tissue of woody plants. BioTechniques 14:734-736.
7. Saiki, R.K., D.H. Gelfand, S. Stoffel, S.J. Scharf, R. Higuchi, G.T. Horn, K.B. Mullis and H.A. Erlich. 1988. Primer directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 239:487-491.
8. Staub, J., J. Bacher and K. Poetter. 1996. Sources of potential errors in the application of random amplified polymorphic DNAs in cucumber. HortScience 31:262-266.
9. Weeden, N.F., G.M. Timmerman, M. Hermmat, B.E. Kneen and M.A. Lodhi. Inheritance and reliability of RAPD markers, p. 12-17. Proceedings of the Symposium: Applications of RAPD Technology to Plant Breeding. 1992 November 1; Minneapolis, MN.
10. Williams, J.G.K., A.R. Kubelik, K.J. Livak, J.A. Rafalski and S.V. Tingey. 1990. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acid Res. 18:6531-6535.