Regulation of gene expression by repressor localization: Biochemical evidence that membrane and DNA binding by the PutA protein are mutually exclusive

Journal of Bacteriology

Volumn 179, Issue 8, 1997, Pages 2788-2791

  Muro Pastor, Alicia M ^a,b   Ostrovsky, Paula ^a,c   Maloy, Stanley ^a  

^a UNIVERSITY OF ILLINOIS AT URBANA CHAMPAIGN   (United States)

^b Isla de la Cartuja (IBVF)   (Spain)

^c UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL PROTEIN;

ARTICLE; BACTERIAL GENETICS; DNA BINDING; GENE EXPRESSION REGULATION; MEMBRANE BINDING; NONHUMAN; PRIORITY JOURNAL; PROTEIN DNA INTERACTION; REGULATORY MECHANISM; REPRESSOR GENE; SALMONELLA TYPHIMURIUM; STRUCTURE ACTIVITY RELATION;

EID: 0030976040     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/jb.179.8.2788-2791.1997     Document Type: Article

References (19)

1. Abrahamson, J. L. A., L. G. Baker, J. T. Stephenson, and J. M. Wood. 1983. Proline dehydrogenase from Escherichia coli K12. Properties of the membrane-associated enzyme. Eur. J. Biochem. 134:77-82.
2. Brown, E. D., and J. M. Wood. 1992. Redesigned purification yields a fully functional PutA protein dimer from Escherichia coli. J. Biol. Chem. 267:13086-13092.
3. Brown, E. D., and J. M. Wood. 1993. Conformational change and membrane association of the PutA protein are coincident with reduction of its FAD cofactor by proline. J. Biol. Chem. 268:8972-8979.
4. Carey, J. 1991. Gel retardation. Methods Enzymol. 208:103-117.
5. Dendinger, S., and W. Brill. 1970. Regulation of proline degradation in Salmonella typhimurium. J. Bacteriol. 103:144-152.
6. Graham, S. B., J. T. Stephenson, and J. M. Wood. 1984. Proline dehydrogenase from Escherichia coli K12. Reconstitution of a functional membrane-association. J. Biol. Chem. 259:2656-2661.
7. Hahn, D. R., R. S. Myers, C. R. Kent, and S. R. Maloy. 1988. Regulation of proline utilization in Salmonella typhimurium: molecular characterization of the put operon, and DNA sequence of the put control region. Mol. Gen. Genet. 213:125-133.
8. Maloy, S. 1987. The proline utilization operon, p. 1513-1519. In F. C. Neidhardt, J. L. Ingraham, K. B. Low, B. Magasanik, M. Schaechter, and H. E. Umbarger (ed.) Escherichia coli and Salmonella typhimurium: cellular and molecular biology, vol. 2. American Society for Microbiology, Washington, D.C.
9. Maloy, S. R., and J. R. Roth. 1983. Regulation of proline utilization in Salmonella typhimurium: characterization of put::Mu d(Ap, lac) operon fusions. J. Bacteriol. 154:561-568.
10. Maloy, S., and V. Stewart. 1993. Autogenous regulation of gene expression. J. Bacteriol. 175:307-316.
11. Menzel, R., and J. Roth. 1981. Purification of the putA gene product. A bifunctional membrane-bound protein from Salmonella typhimurium responsible for the two-step oxidation of proline to glutamate. J. Biol. Chem. 256:9755-9761.
12. Menzel, R., and J. Roth. 1981. Enzymatic properties of the purified putA protein from Salmonella typhimurium. J. Biol. Chem. 256:9762-9766.
13. Miller, V. L., R. K. Taylor, and J. J. Mekalanos. 1987. Cholera toxin transcriptional activator ToxR is a transmembrane DNA binding protein. Cell 48:271-279.
14. Muro-Pastor, A. M., and S. Maloy. 1995. Proline dehydrogenase of the transcriptional repressor PutA is required for induction of the put operon by proline. J. Biol. Chem. 270:9819-9827.
15. Ostrovsky de Spicer, P., K. O'Brien, and S. Maloy. 1991. Regulation of proline utilization in Salmonella typhimurium: a membrane-associated dehydrogenase binds DNA in vitro. J. Bacteriol. 173:211-219.
16. Ostrovsky de Spicer, P., and S. Maloy. 1993. PutA, a membrane-associated flavin dehydrogenase, acts as a redox-dependent transcriptional regulator. Proc. Natl. Acad. Sci. USA 90:4295-4298.
17. Peterson, G. L. 1977. A simplification of the protein assay of Lowry et al. which is more generally applicable. Anal. Biochem. 83:346-356.
18. Vogel, H., and D. Bonner. 1956. Acetylornithinase of Escherichia coli: partial purification and some properties. J. Biol. Chem. 218:97-106.
19. Wood, J. M. 1987. Membrane association of proline dehydrogenase in Escherichia coli is redox dependent. Proc. Natl. Acad. Sci. USA 84:373-377.