The association of nonsense mutation with exon-skipping in hprt mRNA of Chinese hamster ovary cells results from an artifact of RT-PCR

RNA

Volumn 3, Issue 6, 1997, Pages 660-676

  Valentine, Carrie R ^a   Heflich, Robert H ^a  

^a NATIONAL CENTER FOR TOXICOLOGICAL RESEARCH   (United States)

Author keywords

mRNA abundance; quantitative RT PCR; relative enrichment

Indexed keywords

MESSENGER RNA;

ANIMAL CELL; ARTICLE; ARTIFACT; CHO CELL; CONTROLLED STUDY; EXON; NONHUMAN; NONSENSE MUTATION; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA SPLICING;

ANIMALS; ARTIFACTS; CHO CELLS; CODON, NONSENSE; CRICETINAE; EXONS; GENES, REPORTER; HYPOXANTHINE PHOSPHORIBOSYLTRANSFERASE; MUTATION; POLYMERASE CHAIN REACTION; RNA, MESSENGER; SEQUENCE ANALYSIS, DNA; SEQUENCE DELETION; TRANSCRIPTION, GENETIC;

ANIMALIA; CRICETINAE; CRICETULUS GRISEUS;

EID: 0030973067     PISSN: 13558382     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (34)

1. Bach G, Moskowitz SM, Tieu PT, Matynia A, Neufeld EF. 1993. Molecular analysis of Hurler syndrome in Druze and Muslim Arab patients in Israel: Multiple allelic mutations of the IDUA gene in a small geographic area. Am J Human Genetics 53:330-338.
2. Belgrader P, Cheng J, Zhou X, Stephenson LS, Maquat LE. 1994. Mammalian nonsense codons can be as effectors of nuclear mRNA half-life. Mol Cell Biol 14:8219-8228.
3. Belgrader P, Maquat LE. 1994. Nonsense but not missense mutations can decrease the abundance of nuclear mRNA for the mouse major urinary protein, while both types of mutations can facilitate exon skipping. Mol Cell Biol 14:6326-6336.
4. Brennand J, Konecki DS, Caskey CT. 1983. Expression of human and Chinese hamster hypoxanthine-guanine phosphoribosyltransferase cDNA recombinants in cultured Lesch-Nyhan and Chinese hamster fibroblasts. J Biol Chem 258:9593-9596.
5. Caponigro G, Parker R. 1996. Mechanisms and control of mRNA turnover in Saccharomyces cerevisiae. Microbiol Rev 60:233-249.
6. Chasin LA, Urlaub G, Mitchell P, Ciudad C, Barth J, Carothers AM, Steigerwalt T, Brunberger D. 1990. RNA processing mutants at the dihydrofolate reductase locus in Chinese hamster ovary cells. In: Mendelsohn ML, Albertini RJ, eds. Mutation and the environment, part A. Basic mechanisms. New York: Wiley-Liss. pp 295-304.
7. Chen IT, Chasin LA. 1993. Direct selection for mutations affecting specific splice sites in a hamster dihydrofolate reductase minigene. Mol Cell Biol 13:289-300.
8. Cheng J, Fogel-Petrovic M, Maquat LE. 1990. Translation to near the distal end of the penultimate exon is required for normal levels of spliced triosephosphate isomerase mRNA. Mol Cell Biol 10:5215-5225.
9. Chomczynski P, Sacchi N. 1987. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem 162:156-159.
10. Cole J, Skopek TR. 1994. International Commission for Protection Against Environmental Mutagen and Carcinogens. Working paper no. 3. Somatic mutant frequency, mutation rates and mutational spectra in the human population in vivo. Mutation Res 304:33-105.
11. de Boer JG, Drobetsky EA, Grosovsky AJ, Mazur M, Glickman BW. 1989. The Chinese hamster aprt gene as a mutational target. Its sequence and an analysis of direct and inverted repeats. Mutation Res 226:239-244.
12. Dietz HC, Kendzior RJ Jr. 1994. Maintenance of an open reading frame as an additional level of scrutiny during splice site selection. Nature Genetics 8:183-188.
13. Dietz HC, Pyeritz RE. 1995. Mutations in the human gene for fibrillin-1 (FBN1) in the Marfan syndrome and related disorders. Human Mol Genetics 4:1799-1809.
14. Dietz HC, Valle D, Francomano CA, Kendzior RJ Jr, Pyeritz RE, Cutting GR. 1993. The skipping of constitutive exons in vivo induced by nonsense mutations. Science 259:680-683.
15. Gibson RA, Hajianpour A, Murer-Orlando M, Buchwald M, Mathew CG. 1993. A nonsense mutation and exon skipping in the Fanconi anaemia group C gene. Human Mol Genetics 2:797-799.
16. Hennig E, Conney AH, Wei SJC. 1995. Characterization of hprt splicing mutations induced by the ultimate carcinogenic metabolite of benzo[α]pyrene in Chinese hamster V-79 cells. Cancer Res 55: 1550-1558.
17. Hull J, Shackleton S, Harris A. 1994. The stop mutation R553X in the CFTR gene results in exon-skipping. Genomics 19:362-717.
18. Jarolim P, Rubin HL, Brabec V, Palek J. 1995. A nonsense mutation 1669Glu → Ter within the regulatory domain of human erythroid ankyrin leads to a selective deficiency of the major ankyrin isoform (band 2.1) and a phenotype of autosomal dominant hereditary spherocytosis. J Clin Invest 95:941-947.
19. Kessler O, Chasin LA. 1996. Effects of nonsense mutations on nuclear and cytoplasmic adenine phosphoribosyltransferase RNA. Mol Cell Biol 16:4426-4435.
20. Konecki DS, Brennand J, Fuscoe JC, Caskey CT, Chinault AC. 1982. Hypoxanthine-guanine phosphoribosyltransferase genes of mouse and Chinese hamster: Construction and sequence analysis of cDNA recombinants. Nucleic Acids Res 10:6763-6775.
21. Manjanatha MG, Lindsey LA, Mittelstaedt RA, Heflich RH. 1994. Low hprt levels and multiple hprt mRNA species in 6-thioguanine-resistant Chinese hamster cell mutants possessing nonsense mutations. Mutation Res 308:65-75.
22. Maquat L. 1995. When cells stop making sense: Effects of nonsense codons on RNA metabolism in vertebrate cells. RNA 1:453-465.
23. Maquat L. 1996. Defects in RNA splicing and the consequence of shortened translational reading frames. Am J Human Genetics 59:279-286.
24. ot effect" and its consequences. Nucleic Acids Res 24:2080-2086.
25. Naylor JA, Green PM, Rizza CR, Giannelli F. 1993. Analysis of factor VIII mRNA reveals defects in everyone of 28 haemophilia A patients. Human Mol Genetics 2:11-17.
26. Newton RK, Mittelstaedt RA, Heflich RH. 1992a. Analysis of solvent control and 1-nitrosopyrene-induced Chinese hamster ovary cell mutants by Southern and Northern blots and the polymerase chain reaction. Environ Mol Mutagenesis 19:147-155.
27. Newton RK, Mittelstaedt RA, Manjanatha MG, Heflich RH. 1992b. DNA sequence analysis of 1-nitrosopyrene-induced mutations in the hprt gene of Chinese hamster ovary cells. Carcinogenesis 13:819-825.
28. Shapiro MB, Senapathy R. 1987. RNA splice junctions of different classes of eukaryotes: Sequence statistics and functional implications in gene expression. Nucleic Acids Res 15:7155-7174.
29. Steingrimmsdottir H, Rowley G, Dorado G, Cole J, Lehmann AR. 1992. Mutations which alter splicing in the human hypoxanthineguanine phosphoribosyltransferase gene. Nucleic Acids Res 20: 1201-1208.
30. Valentine CR, Heflich RH. 1995. Genomic DNA sequencing of mRNA splicing mutants in the hprt gene of Chinese hamster ovary cells. Environ Mol Mutagenesis 25:85-96.
31. Valentine CR, Heinrich MJ, Chissoe SL, Roe BA. 1994. DNA sequence of direct repeats of the sul I gene of plasmid pSa. Plasmid 32:222-227.
32. Weissensteiner T, Lanchbury JS. 1996. Strategy for controlling preferential amplification and avoiding false negatives in PCR typing. BioTechniques 21:1102-1108.
33. Will K, Dork T, Stuhrmann M, von der Hardt H, Ellemunter H, Tümmler B, Schmidtke J. 1995. Transcript analysis of CFTR nonsense mutations in lymphocytes and nasal epithelial cells from cystic fibrosis patients. Human Mutation 5:210-220.
34. Zhang L-H, Jenssen D. 1991. Site specificity of N-methyl-N-nitrosourea-induced transition mutations in the hprt gene. Carcinogenesis 12:1903-1909.