Cloning DNA fragments between two adjacent/overlapping restriction sites using a 'positive stuffer'

BioTechniques

Volumn 22, Issue 5, 1997, Pages 912-915

  Loukianov, Evgeny ^a   Loukianova, Tanya ^a   Periasamy, Muthu ^a  

^a UNIVERSITY OF CINCINNATI COLLEGE OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DNA FRAGMENT;

ARTICLE; ESCHERICHIA COLI; GENE CASSETTE; MOLECULAR CLONING; NONHUMAN; PLASMID;

ESCHERICHIA COLI;

EID: 0030969981     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/97225st04     Document Type: Article

References (8)

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2. Berg, D.E., J. Daevis, B. Allet and J.D. Rochaix. 1975. Transposition of R-factor genes to bacteriophage. Proc. Natl. Acad. Sci. USA 72:3628-3632.
3. Kaufman, D.L. and G.A. Evans. 1990. Restriction endonuclease cleavage at the termini of PCR products. BioTechniques 9:304-306.
4. Moreira, R.F. and C.J. Noren. 1995. Minimum duplex requirements for restriction enzyme cleavage near the termini of linear DNA fragments. BioTechniques 19:56-59.
5. Rao, R.N. and S.G. Rogers. 1979. Plasmid pKC7: a vector containing ten restriction sites suitable for cloning DNA segments. Gene 7:79-82.
6. Sambrook, J., E.F. Fritsch and T. Maniatis. 1989. Molecular Cloning: A Laboratory Manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
7. Seidman, C.E., K. Struhl and J. Sheen. 1994. Introduction of plasmid DNA into cells, p. 1.8.1.-1.8.8. In F.M. Ausubel, R. Brent, R.E. Kingston, D.D. Moore, J.A. Smith, J.G. Seidman, K. Struhl (Eds.), Current Protocols in Molecular Biology. John Wiley & Sons, New York.
8. Struhl, K. 1994. Construction of hybrid DNA molecules, p. 3.16.1.-3.16.11. In F.M. Ausubel, R. Brent, R.E. Kingston, D.D. Moore, J.A. Smith, J.G. Seidman, K. Struhl (Eds.), Current Protocols in Molecular Biology. John Wiley & Sons, New York.