Isolation and molecular characterization of mRNA transport mutants in Schizosaccharomyces pombe

Molecular Biology of the Cell

Volumn 8, Issue 5, 1997, Pages 825-841

  Azad, Abul Kalam ^a   Tani, Tokio ^a   Shiki, Naruhisa ^a   Tsuneyoshi, Satomi ^a   Urushiyama, Seiichi ^a   Ohshima, Yasumi ^a  

^a KYUSHU UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

CARRIER PROTEIN; MESSENGER RNA; MUTANT PROTEIN; POLYADENYLATED RNA; RNA BINDING PROTEIN;

ARTICLE; CELL VIABILITY; CELLULAR DISTRIBUTION; MOLECULAR CLONING; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN ISOLATION; PROTEIN LOCALIZATION; RNA ANALYSIS; RNA SPLICING; RNA TRANSPORT; SCHIZOSACCHAROMYCES POMBE;

EUKARYOTA; MAMMALIA; SCHIZOSACCHAROMYCES POMBE;

EID: 0030969145     PISSN: 10591524     EISSN: None     Source Type: Journal    
DOI: 10.1091/mbc.8.5.825     Document Type: Article

References (53)

1. Altschul, S.F., Gish, W., Miller, W., Myers, E.W., and Lipman, D.J. (1990). Basic local alignment search tool. J. Mol. Biol. 215, 403-410.
2. Amberg, D.C., Goldstein, A., and Cole, C.N. (1992). Isolation and characterization of RAT1: an essential gene of Saccharomyces cerevisiae required for the efficient nucleocytoplasmic trafficking of mRNA. Gene Dev. 6, 1173-1189.
3. Aris, J.P., and Blobel, G. (1988). Identification and characterization of a yeast nucleolar protein that is similar to a rat liver nucleolar protein. J. Cell Biol. 107, 17-31.
4. Bischoff, F.R., Krebber, H., Kempf, T., Hermes, I., and Ponstingl, H. (1995). Human RanGTPase-activating protein RanGAP1 is a homologue of yeast Rna1p involved in mRNA processing and transport. Proc. Natl. Acad. Sci. USA 92, 1749-1753.
5. Bossie, M.A., DeHoratius, Barcelo, C., G., and Silver, P. (1992). A mutant nuclear protein with similarity to RNA binding proteins interferes with nuclear import in yeast. Mol. Biol. Cell 3, 875-893.
6. + RNA export and in the cytoskeleton. J. Biol. Chem. 270, 7411-7419.
7. Chalfie, M., Tu, Y., Euskirchen, G., Ward, W.W., and Prasher, D.C. (1994). Green fluorescent protein as a marker for gene expression. Science 263, 802-805.
8. Cottarel, G., Beach, D., and Deuschle, U. (1993). Two new multipurpose multicopy Schizosaccharomyces pombe shuttle vectors, pSP1 and pSP2. Curr. Genet. 23, 547-548.
9. Dohmen, R.J., Stappen, R., McGrath, J.P., Forrova, H., Kolarov, J., Gotteau, A., and Varshavsky, A. (1995). An essential yeast gene encoding a homolog of ubiquitin-activating enzyme. J. Biol. Chem., 270, 18099-18109.
10. Earnshaw, W.C., Halligan, B., Cooke, C.A., Heck, M.M.S., and Liu, L.F. (1985). Topoisomerase II is a structural component of mitotic chromosome scaffolds. J. Cell Biol. 100, 1706-1715.
11. Eckner, R., Ellmeier, W., and Birnstiel, M.L. (1991). Mature mRNA 3′ end formation stimulates RNA export from the nucleus. EMBO J. 10, 3513-3522.
12. Fischer, U., Huber, J., Boelens, W.C., Mattaj, I.W., and Lührmann, R. (1995). The HIV-1 Rev activation domain is a nuclear export signal that accesses an export pathway used by specific cellular RNAs. Cell 82, 475-483.
13. Forrester, W., Stutz, F., Rosbash, M., and Wickens, M. (1992). Defects in mRNA 3′-end formation, transcription initiation, and mRNA transport associated with the yeast mutation prp20: possible coupling of mRNA processing and chromatin structure. Genes Dev. 6, 1914-1926.
14. French, B.T., Schumm, D.E., and Webb, T.E. (1987). Active transport of messenger ribonucleoprotein particle in a reconstituted cell-free system. Proc. Natl. Acid. Sci. USA 84, 5163-5166.
15. Gorsch, L.C., Dockendorff, T.C., and Cole, C.N. (1995). A conditional allele of the novel repeat-containing yeast nucleoporin RAT7/ NUP159 causes both rapid cessation of mRNA export and reversible clustering of nuclear pore complexes. J. Cell Biol. 129, 939-955.
16. Guthrie, C., and Fink, G.R (1991). Guide to yeast genetics and molecular biology. Methods Enzymol. 194, 795-823.
17. Hamm, J., and Mattaj, I.W. (1990). Monomethylated cap structures facilitate RNA export from the nucleus. Cell 63, 109-118.
18. + RNA associated with mutation of the Saccharomyces cercvisiae RAT2/NUP120 gene. J. Cell Biol. 131, 1677-1697.
19. Hirano, T., Funahashi, S., Uemura, T., and Yanagida, M. (1986). Isolation and characterization of Schizosaccharomyces pombe cut mutants that block nuclear division but not cytokinesis. EMBO J. 11, 2973-2979.
20. Huang, Y., and Carmichael, G.G. (1996). Role of polyadenylation in nucleocytoplasmic transport of mRNA. Mol. Cell. Biol. 16, 1534-1542.
21. Jarmolowski, A., Boelens, W.C., Izaurralde, E., and Mattaj, I.W. (1994). Nuclear export of different classes of RNA is mediated by specific factors. J. Cell Biol. 124, 627-635.
22. Jentsch, S., Seufert, W., and Hauser, H.-P., (1991). Genetic analysis of the ubiquitin system. Biochim. Biophys. Acta 1089, 127-139.
23. Kadowaki, T., Goldfarb, D., Spitz, L.M., Tartakoff, A.M., and Ohno, M. (1993). Regulation of RNA processing and transport by a nuclear guanine nucleotide release protein and members of the Ras superfamily. EMBO J. 12, 2929-2937.
24. Kadowaki, T., Chen, S., Hitomi, M., Jacobs, E., Kumagai, C., Liang, S., Schneiter, R., Singleton, D., Wisniewska, J., and Tartakoff, A.M. (1994a). Isolation and characterization of Saccharomyces cerevisiae mRNA transport-defective (mtr) mutants. J. Cell Biol. 126, 649-659.
25. Kadowaki, T., Hitomi, M., Chen, S., and Tartakoff, A.M. (1994b). Nuclear mRNA accumulation causes nucleolar fragmentation in yeast mtr2 mutant. Mol. Biol. Cell 5, 1253-1263.
26. + RNA and constitutive clustering of nuclear pore complexes. Mol. Biol. Cell 6, 401-417.
27. Lim, C.R., Kimata, Y., Oka, M., Nomaguchi, K., and Kohno K. (1995). Thermosensitivity of green fluorescent protein fluorescence utilized to reveal nuclear-like compartments in a mutant nucleoporin NSP1. J. Biochem. 118, 13-17.
28. Matsumoto, T., and Beach, D. (1991). Premature initiation of mitosis in yeast lacking RCC1 or an interacting GTPase. Cell 66, 347-360.
29. Maundrell, K. (1993). Thiamine -repressible expression vectors pREP and pRIP for fission yeast. Gene 123, 127-130.
30. McGrath, J.P., Jentsch, S., and Varshavsky, A. (1991). UBA1: an essential yeast gene encoding ubiquitin-activating enzyme. EMBO J. 10, 227-236.
31. Michael, W.M., Choi, M., and Dreyfuss, G. (1995). A nuclear export signal in hnRNP A1: a signal-mediated, temperature-dependent nuclear protein export pathway. Cell 83, 415-422.
32. Moffet, R.B., and Webb, T.E. (1981). Regulated transport of messenger ribonucleic acid from isolated liver nuclei by nucleic acid binding proteins. Biochemistry 20, 3253-3262.
33. Nigg, E.A., Baeuerle, P.A., and Lührmann, R. (1991). Nuclear import-export: in search of signals and mechanisms. Cell 66, 15-22.
34. Nischt, R., Thüroff, E., and Käufer, N.F., (1986). Molecular cloning of a ribosomal gene from the fission yeast Schizosaccharomyces pombe. Curr. Genet. 10, 365-370.
35. Okazaki, K., Okazaki, N., Jinno, S., Tanaka, K., and Okayama, H. (1990). High-frequency transformation and library transducing vectors for cloning mammalian cDNAs by trans-complementation of Schizosaccharomyces pombe. Nucleic Acids Res. 18, 6485-6489.
36. Piñol-Roma, S., and Dreyfuss, G. (1991). Transcription-dependent and transcription-independent nuclear transport of hnRNP proteins. Science 253, 312-314.
37. Piñol-Roma, S., and Dreyfuss, G. (1992). Shuttling of pre-mRNA binding proteins between nucleus and cytoplasm. Nature 355, 730-732.
38. Riedel, N., and Fasold, H. (1992). Role of mRNA transport in post-transcriptional control of gene expression. In: Nuclear Trafficking, ed. C.M. Feldherr, London: Academic Press, 231-290.
39. Sambrook, J., Fritsch, E.F., and Maniatis, T. (1989). Molecular Cloning: A Laboratory Manual, 2nd ed., Cold Spring Harbor, NY: Cold spring Harbor Laboratory Press.
40. Santiago, T.C., Purvis, I.J., Bettany, A.J. E., and Brown, A.J. P. (1986). The relationship between mRNA stability and length in Saccharomyces cerevisiae. Nucleic Acids Res. 14, 8347-8360.
41. Sazer, S. (1996). The search for the primary function of the Ran GTPase continues. Trends Cell Biol. 6, 81-85.
42. Schneiter, R., Kadowaki, T., and Tartakoff, A.M. (1995). mRNA transport in yeast: time to reinvestigate the function of the nucleolus. Mol. Biol. Cell 6, 357-370.
43. Schröder, H.C., Friese, U., Bachmann, M., Zaubitzer, T., and Müller, W.E. G. (1989). Energy requirement and kinetics of transport of poly(A)-free histone mRNA compared to poly(A)-rich mRNA from isolated L-cell nuclei. Eur. J. Biochem. 181, 149-158.
44. Shiokawa, K., and Pogo, A.O. (1974). The role of cytoplasmic membrane in controlling the transport of nuclear messenger RNA and initiation of protein synthesis. Proc. Natl. Acad. Sci. USA 71, 2658-2662.
45. Singleton D.R., Chen, S., Hitomi, M., Kumagai, C., and Tartakoff, A.M. (1995). A yeast protein that bidirectionally affects nucleocytoplasmic transport. J. Cell Sci. 108, 265-272.
46. Tachibana, T., Imamoto, N., Seino, H., Nishimoto, T., and Yoneda, H. (1994). Loss of RCC1 leads to suppression of nuclear protein import in living cells. J. Biol. Chem. 269, 24542-24545.
47. + RNA in heat-shocked yeast cells: implication of nucleolar involvement in mRNA transport. Mol. Biol. Cell 6, 1515-1534.
48. Uemura, T., and Yanagida, M. (1984). Isolation of type I and II DNA topoisomerase mutants from fission yeast: single and double mutants show different phenotypes in cell growth and chromatin organization. EMBO J. 3, 1737-1744.
49. Uemura, T., and Yanagida, M. (1986). Mitotic spindle pulls but fails to separate chromosomes in type II DNA topoisomerase mutants: uncoordinated mitosis. EMBO J. 5, 1003-1010.
50. Urushiyama, S., Tani, T., and Ohshima, Y. (1996). Isolation of novel pre-mRNA splicing mutants of Schizosaccharomyces pombe. Mol. Gen. Genet. 253, 118-127.
51. + gene regulates spindle pole body duplication and has homology to the budding yeast ESP1 gene. Cell 62, 913-925.
52. Vijayraghavan, U., Company, M., and Abelson, J. (1989). Isolation and characterization of pre-mRNA splicing mutants of Saccharomyces cercvisiae. Genes Dev. 3, 1206-1216.
53. Zacksenhaus, E., and Sheinin, R. (1990). Molecular cloning, primary structure and expression of the human X linked A1S9 gene cDNA which complements the tsA1S9 mouse L cell defect in DNA replication. EMBO J. 9, 2923-2929.