Recovery of polypeptides cleaved from purified calmodulin-binding peptide fusion proteins

BioTechniques

Volumn 22, Issue 3, 1997, Pages 451-453

  Vaillancourt, Peter ^a   Simcox, Timothy G ^a   Zheng, Chao Feng ^a  

^a Stratagene   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BLOOD CLOTTING FACTOR 10A; ENTEROPEPTIDASE; HYBRID PROTEIN; SERINE PROTEINASE; THROMBIN;

AMINO ACID SEQUENCE; ARTICLE; CATTLE; ESCHERICHIA COLI; EXPRESSION VECTOR; GENE FUSION; MOLECULAR CLONING; MOLECULAR INTERACTION; MOLECULAR RECOGNITION; NONHUMAN; PROTEIN BINDING; PROTEIN DEGRADATION; PROTEIN PURIFICATION;

BOS TAURUS; ESCHERICHIA COLI;

EID: 0030966427     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/97223bm17     Document Type: Article

References (8)

1. Collins-Racie, L.A., J.M. McColgan, K.L. Grant, E.A. DiBlasio-Smith, J.M. McCoy and E.R. LaVallie. 1995. Production of recombinant bovine enterokinase catalytic subunit in Escherichia coli using the novel secretory fusion partner DsbA. Bio/Technology 13:982-987.
2. Derijard, B., M. Hibi, I.-H. Wu, T. Barrett, B. Su, T. Deng, M. Karin and R.J. Davis. 1994. JNK1: a protein kinase stimulated by UV light and Ha-Ras that binds and phosphorylates the c-Jun activation domain. Cell 76:1025-1037.
3. Grant, D.A.W. and J. Hermon-Taylor. 1979. Hydrolysis of artificial substrates by enterokinase and trypsin and the development of a sensitive specific assay for enterokinase in serum. Biochim. Biophys. Acta 567:207-215.
4. Kyriakis, J.M., P. Banerjee, E. Nikolakaki, T. Dai, E.A. Rubie, M.F. Ahmad, J.U. Avruch and J.R. Woodgett. 1994. The stress-activated protein kinase subfamily of c-jun kinases. Nature 369:156-158.
5. LaVallie, E.R., A. Rehemtulla, L.A. Racie, E.A. DiBlasio, C. Ferenz, K.L. Grant, A. Light and J.M. McCoy. 1993. Cloning and functional expression of a cDNA encoding the catalytic subunit of bovine enterokinase. J. Biol. Chem. 268:23311-23317.
6. Simcox, T.G., C.-F. Zheng, M.E. Simcox and P. Vaillancourt. 1995. Calmodulin binding peptide affinity tag E. coli protein purification system. Strateg. Mol. Cell Biol. 8:40-43.
7. Stofko-Hahn, R.E., D.W. Carr and J.D. Scott. 1992. A single step purification for recombinant proteins. FEBS Lett. 302:274-278.
8. Zheng, C.-F. and T.G. Simcox. 1996. Recombinant c-jun N-terminal kinase (JNK) for studying cellular stress signal transduction in mammalian cells. Strateg. Mol. Biol. 9:20-21.