Automated fluorescence-based screening for mutation by SSCP: Use of universal M13 dye primers for labeling and detection

BioTechniques

Volumn 23, Issue 3, 1997, Pages 358-362

  Boutin, P ^a,b   Hani, E H ^a   Vasseur, F ^a   Roche, C ^a   Bailleul, B ^a   Hager, J ^a   Froguel, P ^a  

^a LILLE UNIVERSITY HOSPITAL   (France)

^b INSTITUT PASTEUR DE LILLE   (France)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; AUTOMATION; DNA DETERMINATION; FLUORESCENCE; GENE MUTATION; GENETIC ANALYSIS; HUMAN; NONHUMAN; NUCLEIC ACID BASE SUBSTITUTION; SCREENING; SINGLE STRAND CONFORMATION POLYMORPHISM;

EID: 0030964569     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/97233bm01     Document Type: Article

References (11)

1. Cassard, A.M., F. Bouillaud, M.G. Mattei, E. Hentz, S. Raimbault, M. Thomas and D. Ricquier. 1993. Human uncoupling gene: structure, comparison with rat gene and assignment to the long arm of chromosome 4. J. Cell. Biochem. 43:255-264.
2. Ellison, J., M. Dean and D. Goldman. 1993. Efficacy of fluorescence-based PCR-SSCP for detection of point mutations. BioTechniques 15:684-691.
3. Froguel, P., M. Vaxillaire, F. Sun, G. Velho, H. Zouali, M.O. Butel, S. Lesage, N. Vionnet et al. 1992. Close linkage of glucokinase locus on chromosome 7p to early-onset non-insulin-dependent diabetes mellitus. Nature 356:162-164.
4. Hager, J., H. Blanché, F. Sun, N. Vionnet, M. Vaxillaire, W. Poller, D. Cohen, P. Czernichow et al. 1994. Six mutations in the glucokinase gene identified in MODY using a nonradioactive, sensitive screening technique. Diabetes 43:730-733.
5. Inoue, H., J. Ferrer, C.M. Welling, S.C. Elbein, M. Hoffman, R. Mayorga, M. Warren-Perry, Y. Zhang et al. 1996. Sequence variants in the sulfonylurea receptor (SUR) gene are associated with NIDDM in Caucasians. Diabetes 45:825-831.
6. Iwahana, H., M. Fujimura, Y. Takahashi, T. Iwabuchi, K. Yoshimoto and M. Itakura. 1996. Multiple fluorescence-based PCR-SSCP analysis using internal fluorescent labeling of PCR products. BioTechniques 21:510-519.
7. Makino, R., H. Yazyu, Y. Kishimoto, T. Sekiya and K. Hayashi. 1992. F-SSCP: fluorecence-based polymerase chain reaction-single-strand conformation polymorphism (PCR-SSCP) analysis. PCR Methods Appl. 2:10-13.
8. Mohabeer, A.J., A.L. Hiti and W.J. Martin. 1991. Non-radioactive single-strand conformation polymorphism (SSCP) using the Pharmacia 'PhastSystem'. Nucleic Acids Res. 19:3154.
9. Oppert, J.M., M.C. Vohl, M. Chagnon, F.T. Dionne, A.M. Cassard-Doulcier, D. Ricquier, L. Perusse and C. Bouchard. 1994. DNA polymorphism in the uncoupling protein (UCP) gene and human body fat. Int. J. Obes. Relat. Metab. Disord. 18:526-531.
10. Orita, M., Y. Suzuki., T. Sekiya and K. Hayashi. 1989. Rapid and sensitive detection of point mutations and DNA polymorphisms using the polymerase chain reaction. Genomics 5:874-879.
11. Sakura, H., N. Wat, V. Horton, H. Millns, R.C. Turner and F.M. Ashcroft. 1996. Sequence variations in the human Kir6.2 gene, a subunit of the beta-cell ATP-sensitive K-channel: no association with NIDDM in white Caucasian subjects or evidence of abnormal function when expressed in vitro. Diabetologia 39:1233-1236.