Chemical modification of amino acid residues in glycerinated Vorticella stalk and Ca2+-induced contractility

Cell Motility and the Cytoskeleton

Volumn 36, Issue 4, 1997, Pages 305-312

  Kono, Rin Ichiro ^a   Ochiai, Tsutomu ^a   Asai, Hiroshi ^a  

^a WASEDA UNIVERSITY   (Japan)

Author keywords

calcium contraction; chemical modification; methionine; spasmoneme; stalk; tryptophan; Vorticella

Indexed keywords

2 HYDROXY 5 NITROBENZYL BROMIDE; AMINO ACID; CALCIUM BINDING PROTEIN; METHIONINE; N BROMOSUCCINIMIDE; TOSYLCHLORAMIDE SODIUM; TRYPTOPHAN;

ARTICLE; CELL ORGANELLE; CHEMICAL MODIFICATION; CILIATE; NONHUMAN; PRIORITY JOURNAL;

2-HYDROXY-5-NITROBENZYL BROMIDE; ANIMALS; BINDING SITES; BROMOSUCCINIMIDE; CALCIUM; CALCIUM-BINDING PROTEINS; CHLORAMINES; CILIOPHORA; GLYCEROL; METHIONINE; MOVEMENT; PROTOZOAN PROTEINS; TOSYL COMPOUNDS; TRYPTOPHAN;

CILIOPHORA; VORTICELLA;

EID: 0030962497     PISSN: 08861544     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0169(1997)36:4<305::AID-CM1>3.0.CO;2-4     Document Type: Article

References (14)

1. Amos, W.B., Routledge, L.M., and Yew, F.F. (1975): Calcium binding proteins in a Vorticellid contractile organelle. J. Cell Sci. 19:203-213.
2. Asai, H., and Morales, M.F. (1965): Stochastic aspects of enzyme inhibition. Biochemistry 4:830-838.
3. Asai, H., Ochiai, T., Fukui, K., Watanabe, M., and Kano, F. (1978): Improved preparation and cooperative calcium contraction of glycerinated Vorticella. J. Biochem. 83:795-798.
4. Asai, H., Nakamura, K., Hosoda, K., and Shindoh, Y. (1995): Amino acid analysis of a 20 KDa calcium binding protein (spasmin) from Carchesium stalks. Proceedings of the 4th Asian Conference on Ciliate Biology and the International Symposium on Cell Motility and Cytogenesis, pp. 171-175.
5. Gagnon, G., Kelly, S., Manganiello, V., Vaugan, M., Strittmatter, W., Hoffmann, A., and Hirata, F. (1980): Modification of calmodulin function by enzymatic carboxylic methylation. Ann. N.Y. Acad. Sci. 356:385-386.
6. Hoffmann-Berling, H. (1958): Der Mechanismus eines neuen, von der Muskelkontraktion verchiedenen Kontraktionszyklus. Biochim. Biophys. Acta 27:247-255.
7. Horton, H.R., and Koshland, D.E. Jr. (1965): A highly reactive colored reagent with selectivity for the tryptophan residue in proteins. 2-hydroxy-5-nitrobenzyl bromide. J. Am. Chem. Soc. 87:1126-1132.
8. Levy, Y.Y., Lai, E.Y., Remillard, S.P., Heintzelman, M.B., and Fulton, C. (1996): Centrin is a conserved protein that forms diverse associations with centrioles and MTOCs in Naegleria and other organisms. Cell Motil. Cytoskelton 33:298-323.
9. Ochiai, T., and Asai, H. (1981): Effect of divalent cations on the loss in contractility of glycerinated Vorticella in high KCl solutions. Comp. Biochem. Physiol. 70A:479-484.
10. Ochiai, T., Asai, H., and Fukui, K. (1979): Hysterisis of contraction-extension cycle of glycerinated Vorticella. J. Protozool. 26:420-425.
11. Shechter, Y., Burstein, Y. and Patchornik, A. (1975): Selective oxidation of methionine residues in proteins. Biochemistry 14:4497-4503.
12. Spande, T.F., Green, N.M., and Witcop, B. (1966): The reactivity toward N-bromosuccinimide of tryptophan in enzymes, zymogens, and inhibited enzymes. Biochemistry 5:1926-1933.
13. 2+-dependent protein modulator of cyclic nucleotide phosphodiesterase. Biochemistry 16:2742-2749.
14. Yamada, K., and Asai, H. (1982): Extraction and some properties of the proteins, spastin B, from the spasmoneme of Carchesium Polypinum. J. Biochem. 91:1187-1195.