Intracellular volume and apparent diffusion constants of perfused cancer cell cultures, as measured by NMR

Magnetic Resonance in Medicine

Volumn 37, Issue 6, 1997, Pages 825-832

  Pilatus, Ulrich ^a   Shim, Hyunsuk ^a   Artemov, Dmitri ^a   Davis, Delphine ^a   Van Zijl, Peter C M ^a   Glickson, Jerry D ^a,b  

^a JOHNS HOPKINS UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b UNIVERSITY OF PENNSYLVANIA   (United States)

Author keywords

Apparent diffusion constant; Cell volume; Diffusion weighted NMR spectroscopy; Microcarrier cell culture

Indexed keywords

CELLS; COLLAGEN; DIFFUSION IN LIQUIDS; DISEASES; METABOLITES; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; SURFACE DIFFUSION; UROLOGY;

CELL VOLUME; DIFFUSION CONSTANT; HUMAN PROSTATE CANCER; INTRACELLULAR METABOLITE CONCENTRATION; INTRACELLULAR VOLUMES; MICROCARRIERS; RESTRICTED DIFFUSION; WATER DIFFUSION CONSTANT;

CONTINUOUS CELL CULTURE;

ANIMAL CELL; ARTICLE; CANCER CELL; CELL VOLUME; DIFFUSION COEFFICIENT; HUMAN; HUMAN CELL; NONHUMAN; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; WATER TRANSPORT;

EID: 0030960014     PISSN: 07403194     EISSN: None     Source Type: Journal    
DOI: 10.1002/mrm.1910370605     Document Type: Article

References (27)

1. P. G. M. van Zijl, C. T. W. Moonen, P. Faustino, J. Pekar, O. Kaplan, Complete separation of intracelluar and extracellular information in NMR spectra of perfused cells by diffusion-weighted spectroscopy. Proc. Natl. Acad. Sci. USA 88, 3228-3232 (1992).
2. P. C. M. van Zijl, D. Davis, C. T. W. Moonen. in "NMR in Physiology and Biomedicine" (R. J. Gillies, Ed.), p. 185, Academic Press, New York, 1994.
3. J. E. Tanner, E. O. Stijskal, Restricted self diffusion of protons in colloidal systems by the pulse gradient, spin echo method. J. Chem. Phys. 49, 1768-1777 (1968).
4. J. Andrasko, Measurement of membrane permability to slowly penetrating molecules by a pulse gradient NMR method. J. Magn. Reson. 21, 479-484 (1976).
5. J. Andrasko, Water diffusion permeability of human eythrocytes studied by a pulse gradient NMR technique. Biochem. Biophys. Acta 428, 304-311 (1976).
6. L. L. Latour, K. Svoboda, P. P. Mitra, C. H. Sotak, Time-dependent diffusion of water in a biological model system. Proc. Natl. Acad. Sci. USA 91. 1229-1233 (1994).
7. J. W. C. van der Veen, P. van Gelderen, J. H. N. Creyghton, W. M. M. J. Bovee, Diffusion in red blood cell suspensions: separation of extracellular and intracellular NMR sodium signal. Magn. Reson. Med. 29, 571-574 (1993).
8. J. S. Schoeniger, N. Aiken, E. Hsu, S. J. Blackband, Relaxation-time and diffusion NMR mircroscopy of single neurons. J. Magn. Reson. B 103, 261-273 (1994).
9. M. Neeman, K. A. Jarret, L. O. Sillerud, J. P. Freyer, Self-diffusion of water in multicellular spheroids measured by magnetic resonance microimaging. Cancer Res. 51, 4072-4079 (1991).
10. Y. S. Schiffenbauer, C. Tempel, R. Abramovitch, G. Meir, M. Neeman, Cyclocreatine accumulation leads to cellular swelling in C6 glioma multicellular speroids: diffusion and one-dimensional chemical shift nuclear magnetic resonance microscopy. Cancer Res. 55, 153-158 (1995).
11. T. Niendorf, U. Flogel, D. G. Norris, D. Leibfritz, Intracellular water diffusion in glioma and neuroblastoma cells, in "Proc., SMR, 1st Annual Meeting, 1993." p. 600.
12. T. Niendorf, U. Flogel, D. G. Norris, D. Leibfritz, Monitoring of cell volume changes during osmotic stress and transient ischemia: the implications for diffusion-weighted imaging, in "Proc., SMR, 2nd Annual Meeting, 1894," p. 1012.
13. J. Karger, H. Pfeiffer, W. Heink, Principle and application of selfdiffusion measurements by nuclear magnetic resonance. Adv. Magn. Reson. 12, 1- 89 (1988).
14. M. D. Herbst, J. H. Goldstein, A review of water diffusion measurements by NMR in human red blood cells. Am. J. Physiol. 256 C1097-C1104 (1989).
15. K. R. Stone, D. D. Mickey, H. Wunderli, G. H. Mickey, D. F. Paulson, Isolation of a human prostate carcinoma cell line (DU145). Int. J. Cancer 21, 274-281 (1978).
16. J. T. Isaacs, W. B. Isaacs, F. J. Feitz, J. Scheres, Establishment and characterization of seven Dunning rat prostate cancer cell lines and their use in developing metastatic ability of prostatic cancer. Prostate 9, 261-281 (1986).
17. P. J. Twentyman, M. J. Brown, J. W. Gray, A. J. Franko, M. A. Scoles, R. F. Kallman, A new mouse model system for (RIF-1) for comparison and end-point studies. J. Natl. Cancer Inst. 64, 595-604 (1980).
18. H. D. Soule, J. Vazuez, A. Long, S. Albert, M. Brennan, A human cell line from a pleural effusion derived from a breast carcinoma. J. Natl. Cancer Inst. 51, 1409-1416 (1973).
19. N. R. Aiken, K. A. McGovern, C. Ng, J. P. Wehrle, J. D. Glickson, 31P NMR spoctroscopic studies of the effect of cyclophosphamide on perfused RIF-1 tumor cells. Magn. Reson. Med. 31, 214-274 (1994).
20. K. A. McGovern, J. S. Schoeninger, J. P. Wehrle, C. E. Ng, J. D. Glickson, Gel entrapment of perfluorucrabons: a fluorine-19 NMR spectroscopic method for monitoring oxygen concentrations in cell perfusion systems. Magn. Reson. Med. 29, 196-204 (1993).
21. J. E. Tanner, Use of the stimulated echo in in NMR diffusion studies. J. Chem. Phys. 52, 2523-2562 (1970).
22. 1H NMR chemical shift selective (CHESS) imaging. Phys. Med. Biol. 30, 341-344 (1985).
23. J. Kärger, Zur Bestimmung der Diffusion in einem Zweibereichsystem mit Hilfe von gepulsten Feldgradienten. Ann. Phys. 24, 1-4 (1969).
24. D. Beckert, H. Pfeifer, Zur Theorie der kernmagnetischen Relaxation in Zweiphasensystemen. Ann. Phys. 16, 262-268 (1965).
25. 1H and 31P NMR studiesof cells extracts: Differences in metabolism between drug-sensitive and drug resistant MCF-7 human breast cancer cells. Biochem. Biophys. Res. Commun. 169, 383-390 (1990).
26. D. G. Stout, P. L. Steponkus, L. D. Bustard, R. M. Cotts, Water permeability of chlorella membranes by nuclear magnetic resonance. Plant Physiol. 62, 146-151 (1978).
27. D. G. Norris, T. Niendorf, D. Leibfritz, Healthy and infarcted brain tissues studied at short diffusion times: the origins of apparent restriction and th reduction in apparent diffusion coefficient, NMR Biomed. 7, 304-310 (1994).