Using HPLC to eliminate quench-interference in fluorogenic-substrate assays of microbial enzyme activity

Soil Biology and Biochemistry

Volumn 29, Issue 2, 1997, Pages 203-205

  Freeman, C ^a  

^a BANGOR UNIVERSITY   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 0030958844     PISSN: 00380717     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0038-0717(96)00305-7     Document Type: Article

References (6)

1. Boschker H. T. S. and Cappenberg T. E. (1994) A sensitive method using 4-methylumbelliferyl-β-cellobiose as a substrate to measure (1,4)-β-Glucanase activity in sediments. Applied and Environmental Microbiology 60, 3592-3596.
2. Burns R. G. (1978) Soil Enzymes. Academic Press, London.
3. Chrost R. J. (1990) Microbial ectoenzymes in aquatic environments. In Aquatic Microbial Ecology; Biochemical and Molecular Approaches (J. Overbeck and R. J. Chrost, Eds), pp. 47-78. Springer, New York.
4. Freeman C., Lock M. A., Marxsen J. and Jones S. E. (1990) Inhibitory effects of high molecular weight dissolved organic matter upon metabolic processes in biofilms from contrasting rivers and streams. Freshwater Biology 24, 159-166.
5. Freeman C., Liska G., Ostle N., Jones S. E. and Lock M. A. (1995) The use of fluorogenic substrates for measuring enzyme activity in peatlands. Plant and Soil 175, 147-152.
6. Sinsabaugh R. L. (1994) Enzymic analysis of microbial pattern and process. Biology and Fertility of Soils 17, 69-74.