Qualitative immunoblot analysis of PKC isoforms expressed in airway smooth muscle

American Journal of Physiology - Lung Cellular and Molecular Physiology

Volumn 272, Issue 4 16-4, 1997, Pages

  Togashi, Hideaki ^a,b   Hirshman, Carol A ^a   Emala, Charles W ^a  

^a DEPARTMENT OF PATHOLOGY   (United States)

^b JOHNS HOPKINS BLOOMBERG SCHOOL OF PUBLIC HEALTH   (United States)

Author keywords

protein kinase C; trachea; Western blot

Indexed keywords

CALCIUM ION; DIACYLGLYCEROL; ISOENZYME; PROTEIN KINASE C;

ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; IMMUNOBLOTTING; MUSCLE TONE; NONHUMAN; PRIORITY JOURNAL; PROTEIN FAMILY; SIGNAL TRANSDUCTION; SMOOTH MUSCLE; SWINE; TRACHEA MUSCLE;

EID: 0030956484     PISSN: 10400605     EISSN: None     Source Type: Journal    
DOI: 10.1152/ajplung.1997.272.4.l603     Document Type: Article

References (27)

1. Akimoto, K., K. Mizuno, S. Osada, S. Tanuma, K. Suzuki, and S. Ohno. A new member of the third class in the protein kinase C family, PKC λ, expressed dominantly in an undifferentiated mouse embryonal carcinoma cell line and also in many tissues and cells. J. Biol. Chem. 269: 12677-12683, 1994.
2. Assender, J. W., E. Kontny, and B. B. Fredholm. Expression of protein kinase C isoforms in smooth muscle cells in various states of differentiation. FEBS Lett. 342: 76-80, 1994.
3. Crabos, M., R. Imber, T. Woodtli, D. Fabbro, and P. Erne. Different translocation of three distinct PKC isoforms with tumor-promoting phorbol ester in human platelets. Biochem. Biophys. Res. Commun. 178: 878-883, 1991.
4. Dekker, L. V., and P. J. Parker. Protein kinase C - a question of specificity. Trends Biochem. Sci. 19: 73-77, 1994.
5. Donnelly, R., M. J. Reed, S. Azhar, and G. M. Reaven. Expression of the major isoenzyme of protein kinase-C in skeletal muscle, nPKC9, varies with muscle type and in response to fructose-induced insulin resistance. Endocrinology 135: 2369-2374, 1994.
6. Donnelly, R., K. Yang, M. B. Omary, S. Azhar, and J. L. Black. Expression of multiple isoenzymes of protein kinase C in airway smooth muscle. Am. J. Respir. Cell Mol. Biol. 13: 253-256, 1995.
7. Haller, H., E. Baur, P. Quass, M. Behrend, C. Lindschau, A. Distler, and F. C. Luft. High glucose concentrations and protein kinase C isoforms in vascular smooth muscle cells. Kidney Int. 47: 1057-1067, 1995.
8. Hug, H., and T. F. Sarre. Protein kinase C isoenzymes: divergence in signal transduction? Biochem. J. 291: 329-343, 1993.
9. Johannes, F., J. Prestle, S. Dieterich, P. Oberhagemann, G. Link, and K. Pfizenmaier. Characterization of activators and inhibitors of protein kinase C. Eur. J. Biochem. 227: 303-307, 1995.
10. Johannes, F., J. Prestle, S. Eis, P. Oberhagemann, and K. Pfizenmaier. PKC μ is a novel, atypical member of the protein kinase C family. J. Biol. Chem. 269: 6140-6148, 1994.
11. Khalil, R. A., and K. G. Morgan. Protein kinase C: a second E-C coupling pathway in vascular smooth muscle. News Physiol. Sci. 7: 10-15, 1992.
12. Knox, A. J. Airway re-modeling in asthma: role of airway smooth muscle. Clin. Sci. 86: 647-652, 1994.
13. Laemmli, U. K. Cleavage of structual proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685, 1970.
14. Langlands, J. M., and J. Diamond. Translocation of protein kinase C in bovine tracheal smooth muscle strips: the effect of methacholine and isoprenaline. Eur. J. Pharmacol. 227: 131-138, 1992.
15. Lehel, C., Z. Olah, H. Mischak, J. F. Mushinski, and W. B. Anderson. Overexpressed protein kinase C-δ and -ε subtypes in NIH 3T3 cells exhibit differential subcellular localization and differential regulation of sodium-dependent phosphate uptake. J. Biol. Chem. 269: 4761-4766, 1994.
16. Leurs, R., M. J. Smit, M. M. Brozius, W. Jansen, A. Bast, and H. Timmerman. Is protein kinase C involved in histamine H1-receptor desensitization? Agents Action Suppl. 33: 393-402, 1991.
17. Mizuno, K., K. Kubo, T. C. Saido, Y. Akita, S. Osada, T. Kuroki, S. Ohno, and K. Suzuki. Structure and properties of a ubiquitously expressed protein kinase C, nPKCδ. Eur. J. Biochem. 202: 931-940, 1991.
18. Nisizuka, Y. The molecular heterogeneity of protein kinase C and its implications for cellular regulation. Nature 334: 661-665, 1988.
19. Ohno, S., K. Mizuno, Y. Adachi, A. Hata, Y. Akita, K. Akimoto, S. Osada, S. Hirai, and K. Suzuki. Activation of novel protein kinases Cδ and Cε upon mitogenic stimulation of quiescent rat 3Y1 fibroblasts. J. Biol. Chem. 269: 17495-17501, 1994.
20. 2+-independent isozymes of protein kinase C mediate exocytosis in antigen-stimulated rat basophilic RBL-2H3 cells. J. Biol. Chem. 268: 1749-1756, 1993.
21. Puceat, M., R. Hilal-Dandan, B. Strulovici, L. L. Brunton, and J. H. Brown. Differential regulation of protein kinase C isoforms in isolated neonatal and adult rat cardiomyocytes. J. Biol. Chem. 269: 16938-16944, 1994.
22. Rasmussen, H., Y. Takuwa, and S. Park. Protein kinase C in the regulation of smooth muscle contraction. FASEB J. 1: 177-185, 1987.
23. Selbie, L. A., C. Schmitz-Peiffer, Y. Sheng, and T. J. Biden. Molecular cloning and characterization of PKC ι, an atypical isoform of protein kinase C derived from insulin-secreting cells. J. Biol. Chem. 268: 24296-24302, 1993.
24. Smith, P. K., R. I. Krohn, G. T. Hermanson, A. K. Mallia, F. H. Gartner, M. D. Provenzano, E. K. Fujimoto, N. M. Goeke, B. J. Olsen, and D. C. Klenk. Measurement of protein using bicinchoninic acid. Anal. Biochem. 150: 76-85, 1985.
25. Terzian, A. R., and R. P. Rubin. Translocation of the a-isozyme of protein kinase C during stimulation of rat parotid acinar cells by phorbol ester and carbachol. Arch. Oral Biol. 38: 1051-1056, 1993.
26. Transduction Laboratories. PKC Technical Bulletin no. S85080. Lexington, KY: Transduction Laboratories, 1996.
27. Wiechelman, K. J., R. D. Braun, and J. D. Fitzpatrick. Investigation of the bicinchoninic acid protein assay: identification of the groups responsible for color formation. Anal. Biochem. 175: 231-237, 1988.