Use of fluorescent analog of CDP-DAG in human skin fibroblasts: Characterization of metabolism, distribution, and application to studies of phosphatidylinositol turnover

Journal of Lipid Research

Volumn 38, Issue 3, 1997, Pages 482-490

  Salman, Michael ^a   Pagano, Richard E ^a  

^a MAYO CLINIC   (United States)

Author keywords

fluorescent lipid analogs; IL 1 ; NBD; PDGF; phospholipid metabolism

Indexed keywords

CYTIDINE DIPHOSPHATE DIGLYCERIDE; DIACYLGLYCEROL KINASE; PHOSPHATIDIC ACID; PHOSPHATIDYLCHOLINE; PHOSPHATIDYLETHANOLAMINE; PHOSPHATIDYLINOSITOL; PHOSPHATIDYLSERINE;

ARTICLE; CELL STIMULATION; CELLULAR DISTRIBUTION; FLUORESCENCE MICROSCOPY; HUMAN; HUMAN CELL; HUMAN TISSUE; LIPID MEMBRANE; LIPID TRANSPORT; LIVER METABOLISM; PHOSPHOINOSITIDE METABOLISM; PHOSPHOLIPID METABOLISM; PRIORITY JOURNAL; SKIN FIBROBLAST;

4-CHLORO-7-NITROBENZOFURAZAN; CELLS, CULTURED; CHROMATOGRAPHY, THIN LAYER; CYCLOHEXANONES; CYTIDINE DIPHOSPHATE DIGLYCERIDES; ENZYME INHIBITORS; FIBROBLASTS; FLUORESCENT DYES; HUMANS; INTERLEUKIN-1; LIPOPROTEIN LIPASE; MEMBRANE LIPIDS; MICROSCOPY, FLUORESCENCE; OLEIC ACID; PALMITIC ACID; PHOSPHATIDIC ACIDS; PHOSPHATIDYLCHOLINES; PHOSPHATIDYLINOSITOLS; PLATELET-DERIVED GROWTH FACTOR; PROPRANOLOL; PYRIMIDINONES; THIAZOLES;

EID: 0030954973     PISSN: 00222275     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (39)

1. Pagano, R. E., and R. G. Sleight. 1985. Defining lipid transport pathways in animal cells. Science. 229: 1051-1057.
2. Hoekstra, D., and J. W. Kok. 1992. Trafficking of glycosphingolipids in eukaryotic cells: sorting and recycling of lipids. Biochim. Biophys. Acta. 1113: 277-294.
3. Rosenwald, A. G., and R. E. Pagano. 1993. Intracellular transport of ceramide and its metabolites at the Golgi complex: insights from short-chain ceramides. Adv. Lipid Res. 26: 101-118.
4. Sleight, R. G., and R. E. Pagano. 1984. Transport of a fluorescent phosphatidylcholine analog from the plasma membrane to the Golgi apparatus. J. Cell Biol. 99: 742-751.
5. Sleight, R. G., and M. N. Abanto. 1989. Difference in intracellular transport of a fluorescent phosphatidylcholine analog in established cell lines. J. Cell Sci. 93: 363-374.
6. Tanaka, Y., and A. J. Schroit. 1983. Insertion of fluorescent phosphatidylserine into the plasma membrane of red blood cells: recognition by autologous macrophages. J. Biol. Chem. 258: 11335-11343.
7. Martin, O. C., and R. E. Pagano. 1987. Transbilayer movement of fluorescent analogs of phosphatidylserine and phosphatidylethanolamine at the plasma membrane of cultured cells: evidence for protein-mediated and ATP-dependent process(es). J. Biol. Chem. 262: 5890-5898.
8. Ting, A. E., and R. E. Pagano. 1990. Detection of phosphatidylinositol-specific phospholipase C at the surface of Swiss 3T3 cells and its potential role in the regulation of cell growth. J. Biol. Chem. 265: 5337-5340.
9. Ting, A. E., and R. E. Pagano. 1991. An improved synthesis of 4-nitrobenz-2-oxa-1,3-diazole analogs of CDP-diacylglycerol and phosphatidylinositol. Chem. Phys. Lipids. 60: 83-91.
10. Pagano, R. E., K. J. Longmuir, and O. C. Martin. 1983. Intracellular translocation and metabolism of a fluorescent phospholipid analogue in cultured fibroblasts. J. Biol. Chem. 258: 2034-2040.
11. Pagano, R. E., and K. J. Longmuir. 1983. Intracellular translocation of fluorescent lipid analogues in cultured mammalian cells. Trends Biochem. Sci. 8: 157-161.
12. Pagano, R. E., and K. J. Longmuir. 1985. Phosphorylation, transbilayer movement, and facilitated intracellular transport of diacylglycerol are involved in the uptake of a fluorescent analog of phosphatidic acid by cultured fibroblasts. J. Biol. Chem. 260: 1909-1916.
13. Lipsky, N. G., and R. E. Pagano. 1983. Sphingolipid metabolism in cultured fibroblasts - microscopic and biochemical studies employing a fluorescent analog of ceramide. Proc. Natl. Acad. Sci. USA. 80: 2608-2612.
14. Lipsky, N. G., and R. E. Pagano. 1985. Intracellular translocation of fluorescent sphingolipids in cultured fibroblasts: endogenously synthesized sphingomyelin and glucocerebroside analogs pass through the Golgi apparatus en route to the plasma membrane. J. Cell. Biol. 100: 27-34.
15. Agranoff, B. W., and W. D. Suomi. 1963. Cytidine diphosphate-DL-dipalmitin. Biochem. Prep. 10: 47-51.
16. Uster, P. S., and R. E. Pagano. 1986. Synthesis and properties of fluorescent analog of cytidine diphosphate-diacylglycerol and phosphatidylinositol. In Enzymes of Lipid Metabolism. L. Freyz, H. Dreyfus, R. Massarelli and S. Gatt, editors. Plenum Publishing Corp., New York. Vol. 2. 493-500.
17. Martin, O. C., and R. E. Pagano. 1994. Internalization and sorting of a fluorescent analog of glucosylceramide to the Golgi apparatus of human skin fibroblasts: utilization of endocytic and non-endocytic mechanisms. J. Cell Biol. 125: 769-781.
18. Koval, M., and R. E. Pagano. 1989. Lipid recycling between the plasma membrane and intracellular compartments: transport and metabolism of fluorescent sphingomyelin analogs in cultured fibroblasts. J. Cell Biol. 108: 2169-2181.
19. Bligh, E. G., and W. J. Dyer. 1959. A rapid method of total lipid extraction and purification. Can. J. Biochem. Physiol. 37: 911-917.
20. Rottem, S. 1980. Membrane lipids of mycoplasma. Biochim. Biophys. Acta. 604: 65-90.
21. Ames, B. N. 1966. Assay of inorganic phosphate, total phosphate and phosphatases. Methods Enzymol. 2: 115-18.
22. Bradford, M. M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72: 248-254.
23. Pagano, R. E., K. J. Longmuir, O. C. Martin, and D. K. Struck. 1981. Metabolism and intracellular localization of a fluorescently labeled intermediate in lipid biosynthesis within cultured fibroblasts. J. Cell Biol. 91: 872-877.
24. Billah, M. M., S. Eckel, T. J. Mullman, R. W. Egan, and M. I. Siegel. 1989. Phosphatidylcholine hydrolysis by phospholipase D determines phosphatidate and diglyceride levels in chemotactic peptide-stimulated human neutrophils. Involvement of phosphatidate phosphohydrolase in signal transduction. J. Biol. Chem. 264: 17069-17077.
25. Balsinde, J., E. Diez, and F. Mollinedo. 1991. Arachidonic acid release from diacylglycerol in human neutrophils. Translocation of diacylglycerol-deacylating enzyme activities from an intracellular pool to plasma membrane upon cell activation. J. Biol. Chem. 266: 15638-15643.
26. Sutherland, C. A., and D. Amin. 1982. Relative activities of rat and dog platelet phospholipase A2 and diglyceride lipase. Selective inhibition of diglyceride lipase by RHC 80267. J. Biol. Chem. 257: 14006-14010.
27. de Chaffoy de Courselles, D., P. Roevens, and H. Van Belle. 1985. R 59022, a diacylglycerol kinase inhibitor. J. Biol. Chem. 260: 15762-15770.
28. Ohtsuka, T., M. Hiura, K. Yoshida, N. Okamura, and S. Ishibashi. 1990. A diacylglycerol kinase inhibitor, R 59022, potentiates superoxide anion production and 46-kDa protein phosphorylation in guinea pig polymorphonuclear leukocytes. J. Biol. Chem. 265: 15418-15423.
29. Kaibuchi, K., Y. Takai, M. Sawamura, M. Hoshijima, T. Fujikura, and Y. Nishizuka. 1983. Synergistic action of protein phosphorylation and calcium mobilization in platelet activation. J. Biol. Chem. 258: 6701-6704.
30. Koval, M., and R. E. Pagano. 1990. Sorting of internalized plasma membrane lipid between recycling and degradative pathways in normal and Niemann-Pick type A fibroblasts. J. Cell Biol. 111: 429-442.
31. Inui, H., Y. Kitami, M. Tani, T. Kondo, and T. Inagami. 1994. Differences in signal transduction between platelet-derived growth factor (PDGF) alpha and beta receptors in vascular smooth muscle cells. PDGF-BB is a potent mitogen, but PDGF-AA promotes only protein synthesis without activation of DNA synthesis. J. Biol. Chem. 269: 30546-30552.
32. Yeo, E. G., A. Kazlauskas, and J. H. Exton. 1994. Activation of phospholipase C-gamma is necessary for stimulation of phospholipase D by platelet-derived growth factor. J. Biol. Chem. 269: 27823-27826.
33. Nakano, H., H. Ohno, and T. Saito. 1994. Activation of phospholipase C-gamma 1 through transfected platelet-derived growth factor receptor enhances interleukin 2 production upon antigen stimulation in a T-cell line. Mol. Cell. Biol. 14: 1213-1219.
34. Yamashita, Y., H. Hasegava-Sasaki, and T. Sasaki. 1991. Suppression by staurosporine of Ca(2+)-mobilization triggered by ligation of antigen-specific receptors on T and B lymphocytes. An essential role of protein tyrosine kinase in the signal transduction. FEBS Lett. 288: 46-50.
35. Nishiyama, T., N. Akutsu, I. Norii, Y. Nakayama, T. Ozawa, and T. Hayashi. 1991. Response to growth factors of human dermal fibroblasts in a quiescent state owing to cell-matrix contact inhibition. Matrix. 11: 71-75.
36. Liu, P., and R. Anderson. 1995. Compartmentalized production of ceramide at the cell surface. J. Biol. Chem. 270: 27179-27185.
37. Galella, G., L. Medini, E. Stragliotto, P. Stafanini, P. Rise, E. Tremoli, and C. Galli. 1992. In human monocytes interleukin 1 stimulates a phospholipase C active on phosphatidylcholine and inactive on phosphatidylinositol. Biochem. Pharmacol. 44: 715-720.
38. Angel, J., O. Colard, F. Chevy, and C. Fournier. 1993. Interleukin 1-mediated phospholipid breakdown and arachidonic acid release in human synovial cells. Arthritis & Rheum. 36: 158-167.
39. 3H]arachidonate incorporation into phospholipids in rat mesangial cells. Life Sci. 50: 2075-2082.