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Volumn 276, Issue 5315, 1997, Pages 1122-1125

A membrane network for nutrient import in red cells infected with the malaria parasite

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACID; LUCIFER YELLOW; NUCLEOSIDE;

EID: 0030953287     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.276.5315.1122     Document Type: Article
Times cited : (203)

References (25)
  • 8
    • 0029022032 scopus 로고
    • S. Lauer, N. Ghori, K. Haldar, Proc. Natl. Acad. Sci. U.S.A. 92, 9181 (1995). PPMP-treated cells were prepared by incubating purified schizonts (36 to 48 hours old) with a 20-fold excess of uninfected red cells for 12 to 16 hours in RPMI 1640 medium containing 10% human serum and 5 μM PPMP. This treatment produced a new generation of PPMP-treated infected red cells that were arrested in the TVM from the onset of ring development. Any residual schizonts were removed by a second Percoll gradient.
    • (1995) Proc. Natl. Acad. Sci. U.S.A. , vol.92 , pp. 9181
    • Lauer, S.1    Ghori, N.2    Haldar, K.3
  • 12
    • 1842305291 scopus 로고    scopus 로고
    • note
    • To examine the uptake of LY after removal of PPMP, we washed cells in culture medium, allowed them to mature under normal growth conditions for 24 hours, and subsequently incubated them with LY (9). To examine the effect of PPMP on the LY channel or transporter activity in the infected red cell membrane, we incubated trophozoite- and schizont-infected red cells with LY (9) in the presence or absence of 5 μM PPMP. The dye could be detected in both treated and control cells, indicating that PPMP does not block the channel or transporter activity at the infected red cell membrane.
  • 14
    • 1842383211 scopus 로고    scopus 로고
    • note
    • 3H]glutamic acid (20 μCi/ml, 377 nM).
  • 19
    • 1842345498 scopus 로고    scopus 로고
    • note
    • -6 M. These experiments were carried out as those described for 5-FO.
  • 21
    • 0025866878 scopus 로고
    • B. Pouvelle et al., Nature 353, 73 (1991).
    • (1991) Nature , vol.353 , pp. 73
    • Pouvelle, B.1
  • 25
    • 1842278411 scopus 로고    scopus 로고
    • note
    • We thank W. I. Li, J. McBride, D. Taylor, and R. Coppel for antibodies to the 45-kD cleft protein, Exp1, HRP1, HRP2, and PfEMP2; A. A. Holder for a knob-forming FCB strain of P. falciparum; R. R. Kopito and S. Mayor for comments on the manuscript; and S. Palmieri and J. VanWye for assistance with the Delta Vision microscope and work station. Supported by NIH grants (AI26670 and AI39071 to K.H., AI26912 and AI01112 to P.K.R.), the MacArthur Foundation (S.L.), and Burroughs Wellcome Fund Awards (New Investigator and New Initiatives in Malaria to K.H.).


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.