Identification and characterization of the nifV-nifZ-nifT gene region from the filamentous cyanobacterium Anabaena sp. strain PCC 7120

Journal of Bacteriology

Volumn 179, Issue 9, 1997, Pages 2930-2937

  Stricker, Olaf ^a   Masepohl, Bernd ^a   Klipp, Werner ^b   Böhme, Herbert ^a  

^a UNIVERSITY OF BONN   (Germany)

^b BIELEFELD UNIVERSITY   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

CITRIC ACID; DNA FRAGMENT; MALIC ACID DERIVATIVE; SYNTHETASE;

ANABAENA; ARTICLE; BACTERIAL GROWTH; BACTERIUM MUTANT; GENE INDUCTION; GENE INSERTION; GENETIC COMPLEMENTATION; GENETIC ORGANIZATION; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL;

EID: 0030952956     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/jb.179.9.2930-2937.1997     Document Type: Article

References (44)

1. Altschul, S. F., W. Gibb, W. Miller, E. W. Myers, and D. J. Lipman. 1990. Basic local alignment search tool. J. Mol. Biol. 215:403-410.
2. Arnold, W., A. Rump, W. Klipp, U. B. Priefer, and A. Pühler. 1988. Nucleotide sequence of a 24,206-base-pair DNA fragment carrying the entire nitrogen fixation gene cluster of Klebsiella pneumoniae. J. Mol. Biol. 203: 715-738.
3. Bauer, C. C., L. Scappino, and R. Haselkorn. 1993. Growth of the cyanobacterium Anabaena on molecular nitrogen: NifJ is required when iron is limited. Proc. Natl. Acad. Sci. USA 90:8812-8816.
4. Böhme, H., and R. Haselkorn. 1988. Molecular cloning and nucleotide sequence analysis of the gene coding for heterocyst ferredoxin from the cyanobacterium Anabaena sp. strain PCC 7120. Mol. Gen. Genet. 214:278-285.
5. Carrasco, C. D., K. S. Ramaswamy, T. S. Ramasubramanian, and J. W. Golden. 1994. Anabaena xisF gene encodes a developmentally regulated site-specific recombinase. Genes Dev. 8:74-83.
6. Casadaban, M. J., and S. N. Cohen. 1980. Analysis of gene control signals by DNA fusion and cloning in Escherichia coli. J. Mol. Biol. 138:179-207.
7. Elhai, J., and C. P. Wolk. 1988. Conjugal transfer of DNA to cyanobacteria. Methods Enzymol. 167:747-754.
8. Elhai, J., and C. P. Wolk. 1988. A versatile class of positive-selection vectors based on the nonviability of palindrome-containing plasmids that allows cloning into long polylinkers. Gene 68:119-138.
9. Elhai, J., and C. P. Wolk. 1990. Developmental regulation and spatial pattern of expression of the structural genes for nitrogenase in the cyanobacterium Anabaena. EMBO J. 9:3379-3388.
10. Golden, S. S., J. Brusslan, and R. Haselkorn. 1987. Genetic engineering of the cyanobacterial chromosome. Methods Enzymol. 153:215-231.
11. Haselkorn, R. 1978. Heterocysts. Annu. Rev. Plant Physiol. 29:319-344.
12. Haselkorn, R., and W. J. Buikema. 1992. Nitrogen fixation in cyanobacteria, p. 166-190. In G. Stacey, R. H. Burris, and H. J. Evans (ed.), Biological nitrogen fixation. Chapman & Hall, New York, N. Y.
13. Hoover, T. R., J. Imperial, J. Liang, P. W. Ludden, and V. K. Shah. 1988. Dinitrogenase with altered substrate specificity results from the use of homocitrate analogues for in vitro synthesis of the iron-molybdenum cofactor. Biochemistry 27:3647-3652.
14. Hoover, T. R., J. Imperial, P. W. Ludden, and V. K. Shah. 1989. Homocitrate is a component of the iron-molybdenum cofactor of nitrogenase. Biochemistry 28:2768-2771.
15. Hoover, T. R., A. D. Robertson, R. L. Cerny, R. N. Hayes, J. Imperial, V. K. Shah, and P. W. Ludden. 1987. Identification of the V factor needed for synthesis of the iron-molybdenum cofactor of nitrogenase as homocitrate. Nature 329:855-857.
16. Imperial, J., R. A. Ugalde, V. K. Shah, and W. J. Brill. 1984. Role of the nifQ gene product in the incorporation of molybdenum into nitrogenase in Klebsiella pneumoniae. J. Bacteriol. 158:187-194.
17. Jacobson, M. R., K. E. Brigle, L. T. Bennett, R. A. Setterquist, M. S. Wilson, V. L. Cash, J. Beynon, W. E. Newton, and D. R. Dean. 1989. Physical and genetic map of the major nif gene cluster from Azotobacter vinelandii. J. Bacteriol. 171:1017-1027.
18. Kim, J., and D. C. Rees. 1994. Nitrogenase and biological nitrogen fixation. Biochemistry 33:389-397.
19. Klipp, W., B. Masepohl, and A. Pühler. 1988. Identification and mapping of nitrogen fixation genes of Rhodobacter capsulatus: duplication of a nifA-nifB region. J. Bacteriol. 170:693-699.
20. Kuritz, T., A. Ernst, T. A. Black, and C. P. Wolk. 1993. High-resolution mapping of genetic loci of Anabaena PCC 7120 required for photosynthesis and nitrogen fixation. Mol. Microbiol. 8:101-110.
21. Madden, M. S., A. M. Krezel, R. M. Allen, P. W. Ludden, and V. K. Shah. 1992. Plausible structure of the iron-molybdenum cofactor of nitrogenase. Proc. Natl. Acad. Sci. USA 89:6487-6491.
22. - mutants of Azotobacter vinelandii. J. Bacteriol. 173:5403-5405.
23. 1SVW gene region: possible role of NifW in homocitrate processing. Mol. Gen. Genet. 238:369-382.
24. Masepohl, B., W. Klipp, and A. Pühler. 1988. Genetic characterization and sequence analysis of the duplicated nifA/nifB gene region of Rhodobacter capsulatus. Mol. Gen. Genet. 212:27-37.
25. Meijer, W. G., and F. R. Tabita. 1992. Isolation and characterization of the nifUSVW-rpoN gene cluster from Rhodobacter sphaeroides. J. Bacteriol. 174: 3855-3866.
26. Moreno-Vivian, C., S. Hennecke, A. Pühler, and W. Klipp. 1989. Open reading frame 5 (ORF5), encoding a ferredoxinlike protein, and nifQ are cotranscribed with nifE, nifN, nifX, and ORF4 in Rhodobacter capsulatus. J. Bacteriol, 171:2591-2598.
27. Moreno-Vivian, C., M. Schmehl, B. Masepohl, W. Arnold, and W. Klipp. 1989. DNA sequence and genetic analysis of the Rhodobacter capsulatus nifENX gene region: homology between NifX and NifB suggests involvement of NifX in processing of the iron-molybdenum cofactor. Mol. Gen. Genet. 216:353-363.
28. Paul, W., and M. Merrick. 1989. The roles of the nifW, nifZ and nifM genes of Klebsiella pneumoniae in nitrogenase biosynthesis. Eur. J. Biochem. 178: 675-682.
29. Razquin, P., S. Schmitz, M. F. Fillat, M. L. Peleato, and H. Böhme. 1994. Transcriptional and translational analysis of ferredoxin and flavodoxin under iron and nitrogen stress in Anabaena sp. strain PCC 7120. J. Bacteriol. 176:7409-7411.
30. Rippka, K., J. Deruelles, J. B. Waterbury, M. Herman, and R. Y. Stanier. 1979. Generic assignments, strain histories and properties of pure cultures of cyanobacteria. J. Gen. Microbiol. 111:1-61.
31. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N. Y.
32. Sanger, F., S. Nicklen, and A. R. Coulson, 1977. DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74:5463-5467.
33. Smoker, J. A., and S. R. Barnum. 1988. Rapid small-scale DNA isolation from filamentous cyanobacteria. FEMS Microbiol. Lett. 56:119-122.
34. Schrautemeier, B., U. Neveling, and S. Schmitz. 1995. Distinct and differently regulated Mo-dependent nitrogen-fixing systems evolved for heterocysts and vegetative cells of Anabaena variabilis ATCC 29413: characterization of the fdxH1/2 gene regions as part of the inf1/2 gene clusters. Mol. Microbiol. 18:357-369.
35. Shah, V. K., T. R. Hoover, J. Imperial, T. D. Paustian, G. P. Roberts, and P. W. Ludden. 1988. Role of nif gene products and homocitrate in the biosynthesis of iron-molybdenum cofactor, p. 115-120. In H. Bothe, F. J. de Bruijn, and W. E. Newton (ed.). Nitrogen fixation: hundred years after. Gustav Fischer Verlag, Stuttgart, Germany.
36. Shah, V. K., J. Imperial, R. A. Ugalde, P. W. Ludden, and W. J. Brill. 1986. In vitro synthesis of the iron-molybdenum cofactor of nitrogenase. Proc. Natl. Acad. Sci. USA 83:1636-1640.
37. Simon, H. M., M. J. Homer, and G. P. Roberts. 1996. Perturbation of nifT expression in Klebsiella pneumoniae has limited effect on nitrogen fixation. J. Bacteriol. 178:2975-2977.
38. Simon, R., U. Priefer, and A. Pühler. 1983. A broad host range mobilization system for in vivo genetic engineering: transposon mutagenesis in gram negative bacteria. Bio/Technology 1:784-791.
39. Smith, B. E., and R. R. Eady. 1992. Metalloclusters of the nitrogenases. Eur. J. Biochem. 205:1-15.
40. Stricker, O., and H. Böhme. Unpublished results.
41. Thiel, T., E. M. Lyons, J. C. Erker, and A. Ernst. 1995. A second nitrogenase in vegetative cells of a heterocyst-forming cyanobacterium. Proc. Natl. Acad. Sci. USA 92:9358-9362.
42. Wolk, C. P., A. Ernst, and J. Elhai. 1994. Heterocyst metabolism and development, p. 769-823. In D. A. Bryant (ed.), The molecular biology of cyanobacteria. Kluwer Academic Publishers, Dordrecht, The Netherlands.
43. Yanisch-Perron, C., J. Vieira, and J. Messing. 1985. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33:103-119.
44. Yura, T., H. Mori, H. Nagai, T. Nagata, A. Ishihama, N. Fujita, K. Isono, K. Mizobuchi, and A. Nakata. 1992. Systematic sequencing of the Escherichia coli genome: analysis of the 0-2.4 min region. Nucleic Acids Res. 20:3305-3308.