Properties and heterologous expression of the glucose transporter GHT1 from Schizosaccharomyces pombe

Yeast

Volumn 13, Issue 3, 1997, Pages 215-224

  Lichtenberg Frate H ^a   Naschen T ^a   Heiland, S ^a   Hofer M ^a  

^a UNIVERSITY OF BONN   (Germany)

Author keywords

glucose transporter gene; heterologous expression; Schizosaccharomyces pombe; substrate accumulation; transport energization

Indexed keywords

GLUCOSE TRANSPORTER;

ARTICLE; BIOACCUMULATION; CONTROLLED STUDY; DNA SEQUENCE; FUNGUS MUTANT; GENE EXPRESSION; GLUCOSE TRANSPORT; NONHUMAN; PRIORITY JOURNAL; SCHIZOSACCHAROMYCES POMBE;

AMINO ACID SEQUENCE; ANIMALS; BASE SEQUENCE; BASIC HELIX-LOOP-HELIX TRANSCRIPTION FACTORS; BLOTTING, NORTHERN; BLOTTING, SOUTHERN; CLONING, MOLECULAR; DEOXYGLUCOSE; ESCHERICHIA COLI; GENE EXPRESSION REGULATION, FUNGAL; GENETIC COMPLEMENTATION TEST; GLUCOSE; HUMANS; MICE; MOLECULAR SEQUENCE DATA; MOLECULAR STRUCTURE; MONOSACCHARIDE TRANSPORT PROTEINS; RESTRICTION MAPPING; SACCHAROMYCES CEREVISIAE; SCHIZOSACCHAROMYCES; SEQUENCE ANALYSIS, DNA; SEQUENCE HOMOLOGY, AMINO ACID; TRANSCRIPTION FACTORS; TRANSFORMATION, GENETIC;

EID: 0030952927     PISSN: 0749503X     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0061(19970315)13:3<215::AID-YEA80>3.0.CO;2-5     Document Type: Article

References (33)

1. Becker, D. M. and Guarente, L. (1991). High efficiency transformation of yeast by electroporation. Methods Enzymol. 194, 182-187.
2. Bergmeyer, H. U. (1962). Methoden der enzymatischen Analyse. Verlag Chemie, Weinheim.
3. Bisson, L. F., Coons, D. M., Kruckeberg, A. L. and Lewis, D. A. (1993). Yeast sugar transporters. Crit. Rev. Biochem. Mol. Biol. 28, 259-308.
4. Dobson, M. J., Tuite, M. F., Roberts, N. A., et al. (1982). Conservation of high efficiency promotor sequences in Saccharomyces cerevisiae. Nucl. Acid. Res. 10, 2625-2637.
5. Dufour, J. P. and Goffeau, A. (1980). Molecular and kinetic properties of the purified plasma membrane ATPase of Schizosaccharomyces pombe. Eur. J. Biochem. 105, 145-154.
6. Fuhrmann, G. F. and Völker, B. (1993). Misuse of graphical analysis in nonlinear sugar transport kinetics by Eadie-Hofstee plots. Biochim. Biophys. Acta 1145, 180-182.
7. +-ATPase from Schizosaccharomyces pombe. J. Biol. Chem. 262, 17549-17555.
8. Ghislain, M. and Goffeau, A. (1991). The pmal and pma2-ATPases from Schizosaccharomyces pombe are functionally interchangeable. J. Biol. Chem. 266, 18276-18279.
9. +-ATPase from Schizosaccharomyces pombe. In Nasim, A., Young, P. G. and Johnson, B. F. (Eds), Molecular Biology of the Fission Yeast. Academic Press, San Diego, pp. 327-429.
10. Goswitz, V. C. and Brooker, R. J. (1995). Structural features of the uniporter/symporter/antiporter superfamily. Protein Sci. 4, 534-537.
11. Hoffman and Winston (1987). A ten minute DNA preparation from yeast efficiently releases autonomous plasmids for transformation of Escherichia coli. Gene 57, 267-272.
12. Höfer, M. and Nassar, F. R. (1987). Aerobic and anaerobic uptake of sugars in Schizosaccharomyces pombe. J. Gen. Microbiol. 133, 2163-2172.
13. Hoever, M., Milbradt, B. and Höfer, M. (1992). D-Gluconate is an alternative growth substrate for cultivation of Schizosaccharomyces pombe. Arch. Microbiol. 157, 191-193.
14. Hoheisel, J. D., Maier, E., Mott, R., et al. (1993). High resolution cosmid and P1 maps spanning the 14Mb genome of the fission yeast S. pombe. Cell 73, 109-120.
15. Ko, C. H., Liang, H. and Gaber, R. F. (1993). Roles of multiple glucose transporters in Saccharomyces cerevisiae. Mol. Cell. Biol. 13, 638-648.
16. Kozak, M. (1981). Possible role of flanking nucleotide in recognition of the AUG initiator codon by eukaryotic ribosomes. Nucl. Acids Res. 9, 5233-5252.
17. Kruckeberg, A. L. and Bisson, L. F. (1990). The HXT2 gene of Saccharomyces cerevisiae is required for high-affinity glucose transport. Mol. Cell. Biol. 10, 5903-5913.
18. Kyte, J. and Doolittle, R. (1982). A simple method for displaying the hydrophobic character of a protein. J. Mol. Biol. 157, 105-132.
19. Lagunas, R. (1993). Sugar transport in Saccharomyces cerevisiae. FEMS Microbiol. Rev. 104, 229-242.
20. Lewis, D. A. and Bisson, L. F. (1991). The HXT1 gene product of Saccharomyces cerevisiae is a new member of the family of hexose transporters. Mol. Cell. Biol. 11, 3804-3813.
21. Marger, M. D. and Saier, M. H. Jr. (1993). A major superfamily of transmembrane facilitators that catalyze uniport, symport and antiport. Trends Biochem. Sci. 18, 13-20.
22. Milbradt, B. and Höfer, M. (1994). Glucose-transport-deficient mutants of Schizosaccharomyces pombe: phenotype, genetics and use for genetic complementation. Microbiol. 140, 2617-2623.
23. Moreno, S., Klar, A. and Nurse, P. (1991). Molecular genetic analysis of fission yeast Schizosaccharomyces pombe. Methods Enzymol. 194, 795-823.
24. Näschen, T., Lichtenberg-Fraté, H., Ludwig, S. and Höfer, M. (1996). Schizosaccharomyces pombe gene GHT1 is highly homologous to other prokaryotic, yeast and higher eukaryotic genes coding for monosaccharide transporters. Folia Microbiol. 41, 109-114.
25. Özcan, S., Freidel, K., Leuker, A. and Ciriacy, M. (1993). Glucose uptake and catabolite repression in dominant HTR1 mutants of Saccharomyces cerevisiae. J. Bacteriol. 175, 5520-5528.
26. Reifenberger, E., Freidel, K. and Ciriacy, M. (1995). Identification of novel HXT genes in Saccharomyces cerevisiae reveals the impact of individual hexose transporters on glycolytic flux. Mol. Microbiol. 16, 157-167.
27. Sambrook, J., Fritsch, E. F. and Maniatis, J, (1989) Molecular Cloning: A Laboratory Manual, 2nd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York.
28. Sauer, N., Caspari, T., Klebl, F. and Tanner, W. (1990a). Functional expression of the Chlorella hexose transporter in Schizosaccharomvces pombe. Proc Natl. Acad. Sci. USA 87, 7949-7952.
29. Sauer, N., Friedländer, K. and Gräml-Wicke, U. (1990b). Primary structure, genomic organization and heterologous expression of a glucose transporter from Arabidopsis thaliana. EMBO J. 9, 3045-3050.
30. Serrano, R. (1989). Structure and function of plasma membrane ATPase. Ann. Rev. Plant Physiol. Plant Mol. Biol. 40, 61-94.
31. +/sugar symport in yeast under conditions of depolarized plasma membrane. J. Bioenerg. Biomembr. 21, 321-334.
32. Walsh, M. C., Smits, H. P., Scholte, M. and van Dam, K. (1994). Affinity of glucose transport in Saccharomyces cerevisiae is modulated during growth on glucose. J. Bacterial 176, 953-958.
33. Weusthuis, R. A., Pronk, J. T., v.d. Broek, P. J. A. and v. Dijken, J. P. (1994). Chemostat cultivation as a tool for studies on sugar transport in yeasts. Microbiol. Rev. 58, 616-630.