Modulation of serotonin transporter activity by a protein kinase C activator and an inhibitor of type 1 and 2A serine/threonine phosphatases

Journal of Neurochemistry

Volumn 68, Issue 6, 1997, Pages 2618-2624

  Sakai, Norio ^a   Sasaki, Keiko ^a   Nakashita, Mayumi ^a   Honda, Shigeru ^a   Ikegaki, Natsu ^a   Saito, Naoaki ^a  

^a KOBE UNIVERSITY   (Japan)

Author keywords

BeWo cell; Calyculin A; Phorbol ester; Protein kinase C; Serotonin transporter; Site directed mutagenesis

Indexed keywords

CARRIER PROTEIN; PHOSPHATASE; PROTEIN KINASE C;

ARTICLE; CANCER CELL CULTURE; CHORIOCARCINOMA; ENZYME ACTIVATION; ENZYME PHOSPHORYLATION; ENZYME REGULATION; HUMAN; HUMAN CELL; PRIORITY JOURNAL; SEROTONIN UPTAKE; SITE DIRECTED MUTAGENESIS;

EID: 0030952538     PISSN: 00223042     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1471-4159.1997.68062618.x     Document Type: Article

References (36)

1. Anderson G. M. and Horne W. C. (1992) Activators of protein kinase C decrease serotonin transport in human platelets. Biochim. Biophys. Acta 1137, 331-337.
2. Apgar J. R. (1991) Regulation of the antigen-induced F-actin response in rat basophilic leukemia cells by protein kinase C. J. Cell Biol. 112, 1157-1163.
3. Apgar J. R. (1995) Activation of protein kinase C in rat basophilic leukemia cells stimulates increased production of phosphatidylinositol 4-phosphate and phosphatidylinositol 4,5-bisphosphate: correlation with actin polymerization. Mol. Biol. Cell 6, 97-108.
4. Blakely R. D., Berson H. E., Fremeau R. J., Caron M. G., Peek M. M., Prince H. K., and Bradley C. C. (1991) Cloning and expression of a functional serotonin transporter from rat brain. Nature 354, 66-70.
5. Casado M., Bendahan A., Zafra F., Danbolt N. C., Aragon C., Gimenez C., and Kanner B. I. (1993) Phosphorylation and modulation of brain glutamate transporters by protein kinase C. J. Biol. Chem. 268, 27313-27317.
6. Corey J. L., Davidson N., Lester H. A., Brecha N., and Quick M. W. (1994) Protein kinase C modulates the activity of a cloned gamma-aminobutyric acid transporter expressed in Xenopus oocytes via regulated subcellular redistribution of the transporter. J. Biol. Chem. 269, 14759-14767.
7. Douglas J. G. and Munro J. F. (1982) Drug treatment and obesity. Pharmacol. Ther. 18, 351-373.
8. Downey G. P., Chan C. K., Lea P., Takai A., and Grinstein S. (1992) Phorbol ester-induced actin assembly in neutrophils: role of protein kinase C. J. Cell Biol. 116, 695-706.
9. Gomeza J., Casado M., Gimenez C., and Aragon C. (1991) Inhibition of high-affinity gamma-aminobutyric acid uptake in primary astrocyte cultures by phorbol esters and phospholipase C. Biochem. J. 275, 435-439.
10. + exchanger. J. Membr. Biol. 90, 1-12.
11. Ha K. S. and Exton J. H. (1993) Activation of actin polymerization by phosphatidic acid derived from phosphatidylcholine in IIC9 fibroblasts. J. Cell Biol. 123, 1789-1796.
12. Hoffman B. J., Mezey E., and Brownstein M. J. (1991) Cloning of a serotonin transporter affected by antidepressants. Science 254, 579-580.
13. Huff R. A., Vaughan R. A., Kuhar M. J., and UhI G. R. (1996) Enhancement of dopamine transporter phosphorylation by phorbol esters: parallels with phorbol-induced decrease in transport. Soc. Neurosci. Abstr. 22, 510.
14. Jayanthi L. D., Ramamoorthy S., Mahesh V. B., Leibach F. H., and Ganapathy V. (1994) Calmodulin-dependent regulation of the catalytic function of the human serotonin transporter in placental choriocarcinoma cells. J. Biol. Chem. 269, 14424-14429.
15. King S. C., Tiller A. A., Chang A. S., and Lam D. M. (1992) Differential regulation of the imipramine-sensitive serotonin transporter by cAMP in human JAr choriocarcinoma cells, rat PC12 pheochromocytoma cells, and C33-14-B1 transgenic mouse fibroblast cells. Biochem. Biophys. Res. Commun. 183, 487-491.
16. Kitayama S., Dohi T., and Uhl G. R. (1994) Phorbol esters alter functions of the expressed dopamine transporter. Eur. J. Pharmacol. 268, 115-119.
17. Krebs E. G. and Beavo J. A. (1979) Phosphorylation-dephosphorylation of enzymes. Annu. Rev. Biochem. 48, 923-959.
18. Kuhar M. J., Roth R. H., and Aghajanian G. K. (1972) Synaptosomes from forebrains of rats with midbrain raphe lesions: selective reduction of serotonin uptake. J. Pharmacol Exp. Ther. 181, 36-45.
19. Miller K. J. and Hoffman B. J. (1994) Adenosine A3 receptors regulate serotonin transport via nitric oxide and cGMP. J. Biol. Chem. 269, 27351-27356.
20. Myers C. L., Lazo J. S., and Pitt B. R. (1989) Translocation of protein kinase C is associated with inhibition of 5-HT uptake by cultured endothelial cells. Am. J. Physiol. 257, L253-L259.
21. Olsnes S., Tonnessen T. I., and Sandvig K. (1986) pH-regulated anion antiport in nucleated mammalian cells. J. Cell Biol. 102, 967-971.
22. Osawa I., Saito N., Koga T., and Tanaka C. (1994) Phorbol ester-induced inhibition of GABA uptake by synaptosomes and by Xenopus oocytes expressing GABA transporter (GAT1). Neurosci. Res. 19, 287-293.
23. Person R. B. and Kemp B. E. (1991) Protein kinase phosphorylation site sequences and consensus specificity motif: tabulations. Methods Enzymol. 200, 62-68.
24. Qian Y., Galli A., Ramamoorthy S., Risso S., DeFelice L. J., and Blakely R. D. (1997) Protein kinase C activation regulates human serotonin transporter in HEK-293 cells via altered cell surface expression. J. Neurosci. 17, 45-57.
25. Ramamoorthy J. D., Ramamoorthy S., Papapetropoulos A., Catravas J. D., Leibach F. H., and Ganapathy V. (1995) Cyclic AMP-independent up-regulation of the human serotonin transporter by staurosporine in choriocarcinoma cells. J. Biol. Chem. 270, 17189-17195.
26. Ramamoorthy S., Cool D. R., Mahesh V. B., Leibach F. H., Melikian H. E., Blakely R. D., and Ganapathy V. (1993) Regulation of the human serotonin transporter. Cholera toxin-induced stimulation of serotonin uptake in human placental choriocarcinoma cells is accompanied by increased serotonin transporter mRNA levels and serotonin transporter-specific ligand binding. J. Biol. Chem. 268, 21626-21631.
27. Rowland N. E. and Carlton J. (1986) Neurobiology of an anorectic drug: fenfluramine. Prog. Neurobiol. 27, 13-62.
28. 3H] carboxymethoxy-3beta-(4-fluorophenyl) tropane to the serotonin transporter. Mol. Pharmacol. 40, 421-426.
29. Rudnick G. and Wall S. C. (1993) Non-neurotoxic amphetamine derivatives release serotonin through serotonin transporters. Mol. Pharmacol. 43, 271-276.
30. Sato K., Adams R., Betz H., and Schloss P. (1995a) Modulation of a recombinant glycine transporter (GLYT1b) by activation of protein kinase C. J. Neurochem. 65, 1967-1973.
31. Sato K., Betz H., and Schloss P. (1995b) The recombinant GABA transporter GAT1 is down-regulated upon activation of protein kinase C. FEBS Lett. 375, 99-102.
32. Tchoumkeu-Nzouessa G. C. and Rebel G. (1996) Activation of protein kinase C down-regulates glial but neuronal taurine uptake. Neurosci. Lett. 206, 61-64.
33. Tian Y., Kapatos G., Granneman J. G., and Bannon M. J. (1994) Dopamine and gamma-aminobutyric acid transporters: differential regulation by agents that promote phosphorylation. Neurosci. Lett. 173, 143-146.
34. Tsakiridis T., Vranic M., and Klip A. (1994) Disassembly of the actin network inhibits insulin-dependent stimulation of glucose transporter and prevents recruitment of glucose transporters to the plasma membrane. J. Biol. Chem. 269, 29934-29942.
35. Vaughan R. A., Huff R. A., Uhl G. R., and Kuhar M. J. (1996) Phorbol ester induced phosphorylation of dopamine transporter in rat striatum. Soc. Neurosci. Abstr. 22, 509.
36. + exchange in Ehrlich ascites tumor cells. J. Biol. Chem. 261, 4529-4534.