Active surfactant in pharyngeal aspirates of term neonates: Lipid biochemistry and surface tension function

European Journal of Clinical Investigation

Volumn 27, Issue 4, 1997, Pages 293-298

  Poets, C F ^a   Arning, A ^a   Bernhard, W ^a   Acevedo, C ^a   Von Der Hardt, H ^a  

^a HANNOVER MEDICAL SCHOOL   (Germany)

Author keywords

High performance liquid chromatography; Pharyngeal aspirates; Phosphatidylcholine molecular species; Phospholipids; Surface tension; Surfactant analysis

Indexed keywords

PHOSPHATIDYLCHOLINE; PHOSPHOLIPID; SURFACTANT;

ARTICLE; ASPIRATION; BIOCHEMISTRY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HUMAN; HUMAN EXPERIMENT; NEWBORN; NORMAL HUMAN; PHARYNX; PRIORITY JOURNAL; SURFACE TENSION; THIN LAYER CHROMATOGRAPHY;

EID: 0030951748     PISSN: 00142972     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2362.1997.1050655.x     Document Type: Article

References (27)

1. Hamm H, Fabel H, Bartsch W. The surfactant system of the adult lung: physiology and clinical perspectives. Clin Invest 1992;70:637-57.
2. Ikegami M, Jacobs H, Jobe A. Surfactant function in respiratory distress syndrome. J Pediatr 1983;102:443-7.
3. Adams FH, Fujiwara T, Emmanouilides GC, Räihä N. Lung phospholipids of human fetuses and infants with and without hyaline membrane disease. J Pediatr 1970;77:833-41.
4. Horowitz S, Watkins RH, Auten LR Jr, Mercier CE, Cheng ER. Differential accumulation of surfactant protein A, B, and C mRNA in two epithelial cell types of hyperoxic lungs. Am J Respir Cell Mol Biol 1991;5:511-15.
5. Sugahara K, Iyama K, Sano K, Morioka T. Differential expressions of surfactant protein SP-A, SP-B, and SP-C mRNA in rats with streptozotocin-induced diabetes demonstrated by in situ hybridization. Am J Respir Cell Mol Biol 1994;11:397-404.
6. Crouch E, Parghi D, Kuan S-F, Persson A. Surfactant protein D: subcellular localization in nonciliated bronchiolar epithelial cells. Am J Physiol 1992;263:L60-66.
7. Akino T. Lipid composition of the surfactant system. In: Robertson B, van Golde LMG, Batenburg JJ, eds. Pulmonary surfactant: from molecular biology to clinical practice. Amsterdam: Elsevier 1992:19-29.
8. Kankaanpää K, Hallman M. Respiratory distress syndrome in very low birth weight infants with occasionally normal surfactant phospholipids. Eur J Pediatr 1982;139:31-4.
9. Kobayashi T, Shido A, Nitta K, Inui S, Ganzuka M, Robertson B. The critical concentration of surfactant in fetal lung liquid at birth. Respir Physiol 1990;80:181-92.
10. Blumenfeld TA, Driscoll JM, James LS. Lecithin/sphingomyelin ratios in tracheal and pharyngeal aspirates in respiratory distress syndrome. J Pediatr 1974;85:403-7.
11. Raymondos K, Martin-Carrera I, Bernhard W, Poets C, von der Hardt H. A method for the isolation and preparation of surfactant from tracheobronchial aspirates in infants and children for quantitative and pulsating bubble analysis. Eur J Clin Invest 1996;26:983-8.
12. Saunders RA, Milner AD. Pulmonary pressure/volume relationships during the last phase of delivery and the first postnatal breaths in human subjects. J Pediatr 1978;93:667-73.
13. Mattox HE, Mackenna J, Hudson CA, Winner JE, Brame RG. Comparison of pharyngeal aspirate and amniotic fluid surfactant composition. Am J Obstet Gynecol 1984;150:132-5.
14. Shelley SA, Paciga JE, Balis JU. Purification of surfactant from lung washings and washings contaminated with blood constituents. Lipids 1977;12:505-10.
15. Enhorning G. Pulsating bubble technique for evaluating pulmonary surfactant. J Appl Physiol 1977;43:198-203.
16. Bernhard W, Linck M, Creutzburg H, Postle AD, Arning A, Martin-Carrera I, Sewing K-F. High-performance liquid chromatographic analysis of phospholipids from different sources with combined fluorescence and ultraviolet detection. Anal Biochem 1994;220:172-80.
17. Postle AD. Method for the sensitive analysis of individual molecular species of phosphatidylcholine by high-performance liquid chromatography using post column fluorescence detection. J Chromatog 1987;419:241-51.
18. Bartlett GR. Phosphorus assay in column chromatography. J Biol Chem 1959;234:466-8.
19. Folch J, Lees M, Stanley GHS. A simple method for the isolation and purification of total lipids from animal tissues. J Biol Chem 1957;226:497-509.
20. Bernhard W, Müller B, von Wiehert P. β-Adrenergic priming of rats in vivo modulates the effect of β-agonist in vitro on surfactant phospholipid metabolism of isolated lungs. Eur J Clin Invest 1994;24:393-9.
21. Weller PH, Jenkins PA, Gupta J, Baum JD. Pharyngeal lecithin/ sphingomyelin ratios in newborn infants. Lancet 1976;i:12-15.
22. Stoffel W, Chu F, Ahrens EH Jr. Analysis of long-chain fatty acids by gas-liquid chromatography. Micromethod for the preparation of methyl esters. Anal Chem 1959;31:307-8.
23. Obladen M, Merritt TA, Gluck L. Acceleration of pulmonary surfactant maturation in stressed pregnancies: a study of neonatal lung effluent. Am J Obstet Gynecol 1979;135:1079-85.
24. Shelley SA, Balis JU, Paciga JE, Espinoza CG, Richman AV. Biochemical composition of adult human lung surfactant. Lung 1982;160:195-206.
25. Barrow RE. Chemical structure of phospholipids in the lungs and airways of sheep. Respir Physiol 1990;79:1-8.
26. Bernhard W, von der Hardt H. Bronchial surfactant: phospholipid classes and phosphatidylcholine molecular species as indicators of its alveolar origin. Appl Cardiopulm Physiol 1995;5:6-7.
27. Ashton MR, Postle AD, Hall MA, Smith SL, Kelly FJ, Normand ICS. Phosphatidylcholine composition of endotracheal tube aspirates of neonates and subsequent respiratory disease. Arch Dis Child 1992;67:378-82.