Acidosis induces hyperpermeability in Caco-2BBe cultured intestinal epithelial monolayers

American Journal of Physiology - Gastrointestinal and Liver Physiology

Volumn 272, Issue 5 35-5, 1997, Pages

  Menconi, Michael J ^a   Salzman, Andrew L ^a   Unno, Naoki ^a   Ezzell, Robert M ^a   Casey, Diane M ^a   Brown, Daniel A ^a   Tsuji, Yoshifumi ^a   Fink, Mitchell P ^a,b  

^a HARVARD MEDICAL SCHOOL   (United States)

^b BETH ISRAEL DEACONESS MEDICAL CENTER   (United States)

Author keywords

ATP; Dextran; Epithelium; Hypercarbia; Intestine; Tight junction

Indexed keywords

ACIDOSIS; ARTICLE; HUMAN; HUMAN CELL; INTESTINE EPITHELIUM; INTESTINE MUCOSA PERMEABILITY; PRIORITY JOURNAL; SWINE; TIGHT JUNCTION;

EID: 0030950581     PISSN: 01931857     EISSN: None     Source Type: Journal    
DOI: 10.1152/ajpgi.1997.272.5.g1007     Document Type: Article

References (39)

1. Adams, R. B., S. M. Planchon, and J. K. Roche. IFN-γ modulation of epithelial barrier function: time course, reversibility, and site of cytokine binding. J. Immunol. 150: 2356-2363, 1993.
2. Antonsson, J. B., C. C. Boyle, K. L. Kruithoff, H. Wang, E. Sacristan, H. R. Rothschild, and M. P. Fink. Validation of tonometric measurement of gut intramural pH during endotoxemia and mesenteric occlusion in pigs. Am. J. Physiol. 259 (Gastrointest. Liver Physiol. 22): G519-G523, 1990.
3. Bjork, M., and B. Hedberg. Early detection of major complications after abdominal aortic surgery: predictive value of sigmoid colon and gastric intramucosal pH monitoring. Br. J. Surg. 81: 25-30, 1994.
4. Carpenter, J. F., and S. C. Hand. Reversible dissociation and inactivation of phosphofructokinase in ischemic rat heart. Am. J. Physiol. 250 (Regulatory Integrative Comp. Physiol. 19): R512-R518, 1986.
5. Chang, M. C., M. L. Cheatham, L. D. Nelson, E. J. Rutherford, and J. A. Morris, Jr. Gastric tonometry supplements information provided by systemic indicators of oxygen transport. J. Trauma 37: 488-494, 1994.
6. Cosson, P., J. de Curtis, J. Pouyssegur, G. Griffiths, and J. Davoust. Low cytoplasmic pH inhibits endocytosis and transport from the trans-Golgi network to the cell surface. J. Cell Biol. 108: 377-387, 1989.
7. Duffey, M. E., B. Hainau, S. Ho, and C. J. Bentzel. Regulation of epithelial tight junction permeability by cyclic AMP. Nature 294: 451-453, 1981.
8. Graham, R. D., and M. J. Karnovsky. The early stages of absorption of injected horseradish peroxidase in the proximal tubules of the mouse kidney: ultrastructural chemistry by a new technique. J. Histochem. Cytochem. 14: 291-300, 1966.
9. Gutierrez, G., C. Clark, S. D. Brown, K. Price, L. Ortiz, and C. Nelson. Effect of dobutamine on oxygen consumption and gastric mucosal pH in septic patients. Am. J. Respir. Crit. Care Med. 150: 324-329, 1994.
10. Hidalgo, I. J., T. J. Raub, and R. T. Borchardt. Characterization of human colonic carcinoma cell line (Caco-2) as a model system of intestinal epithelial permeability. Gastroenterology 96: 736-749, 1989.
11. Hingson, D. J., and J. M. Diamond. Comparison of nonelectrolyte permeability patterns in several epithelia. J. Membr. Biol. 10: 93-135, 1972.
12. Krugliak, P., D. Hollander, K. Le, T. Ma, V. D. Dadufalza, and K. D. Katz. Regulation of polyethylene glycol 400 intestinal permeability by endogenous and exogenous prostanoids. Influon non-steroidal anti-inflammatory drugs. Gut 31: 417-421, 1990.
13. Ladino, C., E. E. Schneeberger, C. A. Rabito, and R. D. Lynch. Inhibition of adenine nucleotide synthesis: effect on tight junction structure and function of clone 4 MDCK cells. Eur. J. Cell Biol. 55: 217-224, 1991.
14. Li, C., and M. J. Poznanasky. Effect of FCCP on tight junction permeability and cellular distribution of ZO-1 protein in epithelial (MDCK) cells. Biochim. Biophys. Acta 1030: 297-300, 1990.
15. Lowry, O. H., N. J. Rosebrough, A. L. Farr, and R. J. Randall. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193: 265-275, 1951.
16. Madara, J. L. Increases in guinea pig small intestinal transepithelial resistance induced by osmotic loads are accompanied by rapid alterations in absorptive cell tight-junction structure. J. Cell. Biol. 97: 125-136, 1983.
17. Madara, J. L. Loosening tight junctions: lessons from the intestine. J. Clin. Invest. 83: 1089-1094, 1989.
18. Madara, J. L., and J. Stafford. Interferon-γ directly affects barrier function of cultured intestinal epithelial monolayers. J. Clin. Invest. 83: 724-727, 1989.
19. Mandel, L. J., R. Bacallao, and G. Zampighi. Uncoupling of the molecular "fence" and paracellular "gate" functions in epithelial tight junctions. Nature 361: 552-555, 1993.
20. Matthews, J. B., J. A. Smith, K. J. Tally, M. J. Menconi, H. Nguyen, and M. P. Fink. "Chemical hypoxia" increases junctional permeability and activates chloride transport in human intestinal epithelial monolayers. Surgery 116: 150-158, 1994.
21. McRoberts, J. A., R. Aranda, N. Riley, and H. Kang. Insulin regulates the paracellular permeability of cultured intestinal epithelial cell monolayers. J. Clin. Invest. 85: 1127-1134, 1990.
22. McRoberts, J. A., and N. E. Riley. Regulation of T84 cell monolayer permeability by insulin-like growth factors. Am. J. Physiol. 262 (Cell Physiol. 31): C207-C213, 1992.
23. Nylander, O., P. Kvietys, and D. N. Granger. Effects of hydrochloric acid on duodenal and jejunal mucosal permeability in the rat. Am. J. Physiol. 257 (Gastrointest. Liver Physiol. 20): G653-G660, 1989.
24. Riehl, T. E., and W. F. Stenson. Mechanisms of transit of lipid mediators of inflammation and bacterial peptides across intestinal epithelia. Am. J. Physiol. 267 (Gastrointest. Liver Physiol. 30): G687-G695, 1994.
25. 2+ in lymphocytes. J. Cell. Biol. 95: 189-196, 1982.
26. Salzman, A. L., M. J. Menconi, N. Unno, R. M. Ezzell, D. M. Casey, P. K. Gonzalez, and M. P. Fink. Nitric oxide dilates tight junctions and depletes ATP in cultured Caco-2BBe intestinal epithelial monolayers. Am. J. Physiol. 268 (Gastrointest. Liver Physiol. 31): G361-G373, 1995.
27. 2. Crit. Care Med. 22: 126-134, 1994.
28. Salzman, A. L., H. Wang, P. S. Wollert, T. J. VanderMeer, C. C. Compton, A. G. Denenberg, and M. P. Fink. Endotoxin-induced ileal mucosal hyperpermeability in pigs: role of tissue acidosis. Am. J. Physiol. 266 (Gastrointest. Liver Physiol. 29): G633-G646, 1994.
29. Salzman, A. L., P. S. Wollert, H. Wang, M. J. Menconi, M. E. Youssef, C. C. Compton, and M. P. Fink. Intraluminal oxygenation ameliorates ischemia/reperfusion-induced gut mucosal hyperpermeability in pigs. Circ. Shock 40: 37-46, 1993.
30. Stenson, W. F., R. A. Easom, T. E. Riehl, and J. Turk. Regulation of paracellular permeability in Caco-2 cell monolayers by protein kinase C. Am. J. Physiol. 265 (Gastrointest. Liver Physiol. 28): G955-G962, 1993.
31. Straus, W. Imidazole increases the sensitivity of the cytochemical reaction for peroxidase with diaminobenzidine at neutral pH. J. Histochem. Cytochem. 30: 491-493, 1982.
32. Suleymanlar, G., H. Z. Zhou, M. McCormack, N. Elkins, R. Kucera, O. K. Reiss, and J. I. Shapiro. Mechanism of impaired energy metabolism during acidosis: role of oxidative metabolism. Am. J. Physiol. 262 (Heart Circ. Physiol. 31): H1818-H1822, 1992.
33. Trivedi, B., and W. H. Danforth. Effect of pH on the kinetics of frog muscle phosphofructokinase. J. Biol. Chem. 241: 4110-4114, 1966.
34. Unno, N., M. J. Menconi, A. L. Salzman, M. Smith, S. Hagen, Y. Ge, R. M. Ezzell, and M. P. Fink. Hyperpermeability and ATP depletion induced by chronic hypoxia or glycolytic inhibition in Caco-2BBe monolayers. Am. J. Physiol. 270 (Gastrointest. Liver Physiol. 33): G1010-G1021, 1996.
35. Unno, N., M. J. Menconi, M. Smith, and M. P. Fink. Nitric oxide mediates interferon-γ-induced hyperpermeability in cultured human intestinal epithelial monolayers. Crit. Care Med. 23: 1170-1176, 1995.
36. Van Os, C. H., M. D. de Jong, and J. F. G. Siegers. Dimensions of polar pathways through rabbit gallbladder epithelium. J. Membr. Biol. 15: 363-382, 1974.
37. Whitaker, J. E., R. P. Haugland, and F. G. Prendergast. Spectral and photophysical studies of benzo[c]xanthene dyes: dual emission pH sensors. Anal. Biochem. 194: 330-344, 1991.
38. Williams, S. K., M. A. Matthews, and R. C. Wagner. Metabolic studies on the micropinocytotic process in endothelial cells. Microvasc. Res. 18: 175-184, 1979.
39. Winter, M., J. S. Wilson, K. Bedell, and D. M. Shasby. The conductance of cultured epithelial cell monolayers: oxidants, adenosine triphosphate, and phorbol dibutyrate. Am. J. Respir. Cell. Mol. Biol. 2: 355-364, 1990.