Mutations affecting substrate specificity of the Bacillus subtilis multidrug transporter Bmr

Journal of Bacteriology

Volumn 179, Issue 7, 1997, Pages 2189-2193

  Klyachko, Katya A ^a   Schuldiner, Shimon ^b   Neyfakh, Alexander A ^a  

^a UNIVERSITY OF ILLINOIS AT CHICAGO   (United States)

^b HEBREW UNIVERSITY OF JERUSALEM   (Israel)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACID SUBSTITUTION; ARTICLE; BACILLUS SUBTILIS; BACTERIUM MUTANT; CROSS RESISTANCE; ELECTRIC POTENTIAL; ENZYME SPECIFICITY; ENZYME SUBSTRATE; GENE MUTATION; MULTIDRUG RESISTANCE; NONHUMAN; PRIORITY JOURNAL; PROTEIN FAMILY; PROTEIN TRANSPORT; TRANSPORT KINETICS;

BACILLUS SUBTILIS; BACTERIA (MICROORGANISMS);

EID: 0030949566     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/jb.179.7.2189-2193.1997     Document Type: Article

References (19)

1. Ahmed, M., C. M. Borsch, A. A. Neyfakh, and S. Schuldiner. 1993. Mutants of the Bacillus subtilis multidrug transporter Bmr with altered sensitivity to the antihypertensive alkaloid reserpine. J. Biol. Chem. 268:11086-11089.
2. Ahmed, M., C. M. Borsch, S. S. Taylor, N. Vazquez-Laslop, and A. A. Neyfakh. 1994. A protein that activates expression of a multidrug efflux transporter upon binding the transporter substrates. J. Biol. Chem. 269: 28506-28513.
3. Ahmed, M., L. Lyass, P. N. Markham, S. S. Taylor, N. Vazquez-Laslop, and A. A. Neyfakh. 1995. Two highly similar multidrug transporters of Bacillus subtilis whose expression is differentially regulated. J. Baeteriol. 177:3904-3910.
4. Bron, S. 1990. Plasmids, p. 75-174. In C. R. Harwood and S. M. Cutting (ed.), Molecular biological methods for Bacillus. John Wiley & Sons, Chichester, United Kingdom.
5. Choi, K. H., C. J. Chen, M. Kriegler, and I. B. Roninson. 1988. An altered pattern of cross-resistanee in multidrug-resistant human cells results from spontaneous mutations in the MDR1 (P-glycoprotein) gene. Cell 53:519-529.
6. Devine, S. E., and P. W. Melera. 1994. Diversity of multidrug resistance in mammalian cells. J. Biol. Chem. 269:6133-6139.
7. Gottesman, M. M., and I. Pastan. 1993. Biochemistry of multidrug resistance mediated by the multidrug transporter. Annu. Rev. Biochem. 62:385-427.
8. Gros, P., R. Dhir, J. Croop, and F. Talbot. 1991. A single amino acid substitution strongly modulates the activity and substrate specificity of the mouse mdr1 and mdr3 drug efflux pumps. Proc. Natl. Acad. Sci. USA 88:7289-7293.
9. Le Grice, S. F., and A. L. Sonenshein. 1982. Interaction of Bacillus subtilis RNA polymerase with a chromosomal promoter. J. Mol. Biol. 162:551-564.
10. Lewis, K. 1994. Multidrug-resistance pumps in bacteria: variations on a theme. Trends Biochem. Sci. 19:119-123.
11. Loo, T. W., and D. M. Clarke. 1993. Functional consequences of phenylalanine mutations in the predicted transmembrane domain of P-glycoprotein. J. Biol. Chem. 268:19965-19972.
12. Neyfakh, A. A., V. E. Bidnenko, and L. B. Chen. 1991. Efflux-mediated multidrug resistance in Bacillus subtilis: similarities and dissimilarities with the mammalian system. Proc. Natl. Acad. Sci. USA 88:4781-4785.
13. Neyfakh, A. A., C. M. Borsch, and G. W. Kaatz. 1993. Fluoroquinolone resistance protein NorA of Staphylococcus aureus is a multidrug efflux transporter. Antimicrob. Agents Chemother. 37:128-129.
14. Nikaido, H. 1994. Prevention of drug access to bacterial targets: permeability barriers and active efflux. Science 264:382-388.
15. Nikaido, H. 1996. Mullidrug efflux pumps of gram-negative bacteria. J. Baeteriol. 178:5853-5859.
16. Paulsen, I. T., M. H. Brown, T. G. Littlejohn, B. A. Mitchell, and R. A. Skurray. 1996. Multidrug resistance proteins QacA and QacB from Staphylococcus aureus: membrane topology and identification of residues involved in substrate specificity. Proc. Natl. Acad. Sci. USA 93:3630-3635.
17. Paulsen, I. T., M. H. Brown, and R. A. Skurray. 1996. Proton-dependent multidrug efflux systems. Microbiol. Rev. 60:575-608.
18. Roepe, P. D. 1995. The role of the MDR protein in altered drug translocation across tumor cell membranes. Biochim. Biophys. Acta 1241:385-405.
19. Timmers, K., and R. Sternglanz. 1978. Ionization and divalent cation dissociation constants of nalidixic and oxolinic acids. Bioinorg. Chem. 9:145-155.